Restriction map for
pF1KSDA1350
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BanII BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1808 GTCTAC GT^[AC][GT]AC
AflIII 1767 ACATGT A^C[GA][CT]GT
AlwNI 2036 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2365 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 758 GTGCTC G[AT]GC[AT]^C
AspI 1464 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1278 GGATCC G^GATCC
BanI 2449 GGTGCC G^G[CT][GA]CC
BciVI 2553 GGATAC GTATCC(6/5)
BglII 2131 AGATCT A^GATCT
BmyI 758 GTGCTC G[AGT]GC[ACT]^C
BpmI 1037 CTCCAG CTGGAG(16/14)
BsgI 998 CTGCAC GTGCAG(16/14)
BsiHKAI 758 GTGCTC G[AT]GC[AT]^C
BsiYI 1419 CCTTGTAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1419 CCTTGTAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 758 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 1770 TGTACA T^GTACA
BspDI 1531 ATCGAT AT^CGAT
BspHI 1710 TCATGA T^CATGA
BspLU11I 1767 ACATGT A^CATGT
BspMI 504 GCAGGT ACCTGC(4/8)
BsrGI 1770 TGTACA T^GTACA
BstAPI 422 GCATTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1531 ATCGAT AT^CGAT
DrdI 1223 GACAGCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1968 GAAGAG CTCTTC(1/4)
EcoNI 1419 CCTTGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1714 GAATTC G^AATTC
EcoRV 1555 GATATC GAT^ATC
EcoT14I 1820 CCAAGG C^C[AT][AT]GG
HapII 1152 CCGG C^CGG
HpaII 1152 CCGG C^CGG
Ksp632I 1968 GAAGAG CTCTTC(1/4)
MfeI 152 CAATTG C^AATTG
MspI 1152 CCGG C^CGG
MunI 152 CAATTG C^AATTG
NdeI 2341 CATATG CA^TATG
NspI 1767 ACATGT [GA]CATG^[CT]
PflFI 1464 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1710 TCATGA T^CATGA
SfcI 75 CTATAG C^T[GA][CT]AG
SspBI 1770 TGTACA T^GTACA
SspI 649 AATATT AAT^ATT
StuI 1416 AGGCCT AGG^CCT
StyI 1820 CCAAGG C^C[AT][AT]GG
Tth111I 1464 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1990 CCATCTTCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2365 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AvaII 697 GGACC G^G[AT]CC
1824 GGACC
BalI 99 TGGCCA TGG^CCA
1297 TGGCCA
BseRI 2045 CTCCTC GAGGAG(10/8)
2418 CTCCTC
BsmAI 2506 GAGAC GTCTC(1/5)
412 GTCTC
BssKI 232 CCTGG ^CC[ACGT]GG
1039 CCAGG
BstNI 232 CCTGG CC^[AT]GG
1039 CCAGG
BstYI 1278 GGATCC [GA]^GATC[CT]
2131 AGATCT
Cac8I 1696 GCAGGC GC[ACGT]^[ACGT]GC
2125 GCCAGC
EaeI 99 TGGCCA [CT]^GGCC[GA]
1297 TGGCCA
EcoRII 232 CCTGG ^CC[AT]GG
1039 CCAGG
EcoT22I 420 ATGCAT ATGCA^T
2459 ATGCAT
MaeII 1623 ACGT A^CGT
1946 ACGT
MflI 1278 GGATCC [GA]^GATC[CT]
2131 AGATCT
MluNI 99 TGGCCA TGG^CCA
1297 TGGCCA
MscI 99 TGGCCA TGG^CCA
1297 TGGCCA
MspA1I 513 CAGCTG C[AC]G^C[GT]G
1128 CAGCTG
MvaI 232 CCTGG CC^[AT]GG
1039 CCAGG
NsiI 420 ATGCAT ATGCA^T
2459 ATGCAT
PvuII 513 CAGCTG CAG^CTG
1128 CAGCTG
ScrFI 232 CCTGG CC^[ACGT]GG
1039 CCAGG
XhoII 1278 GGATCC [GA]^GATC[CT]
2131 AGATCT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BclI BfaI BpuAI
BsaJI BsmFI BsmI BsrI BstF5I
Cfr13I DdeI DpnII DraI FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I