Restriction map for
pF1KSDA1348
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbsI BcgI BciVI BfaI BfrI
BglI BglII BlnI BpuAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BspMI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
StuI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 68 GTAGAC GT^[AC][GT]AC
AccII 1415 CGCG CG^CG
AcyI 1018 GGCGTC G[GA]^CG[CT]C
AflIII 916 ACACGT A^C[GA][CT]GT
AlwI 23 GGATC GGATC(4/5)
AocI 1177 CCTGAGG CC^T[ACGT]AGG
AseI 690 ATTAAT AT^TAAT
AsnI 690 ATTAAT AT^TAAT
Asp700I 250 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 128 CCCGGG C^[CT]CG[GA]G
AvaII 363 GGACC G^G[AT]CC
BclI 1421 TGATCA T^GATCA
BsaHI 1018 GGCGTC G[GA]^CG[CT]C
BsaI 1329 GAGACC GGTCTC(1/5)
BsmI 1085 GCATTC GAATGC(1/-1)
BsoBI 128 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1514 TGTACA T^GTACA
BsrBI 1468 GAGCGG CCGCTC(-3/-3)
BsrFI 365 ACCGGC [GA]^CCGG[CT]
BsrGI 1514 TGTACA T^GTACA
BssSI 1390 CACGAG CACGAG(-5/-1)
BstUI 1415 CGCG CG^CG
BstXI 816 CCATGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 23 GGATCT [GA]^GATC[CT]
Bsu36I 1177 CCTGAGG CC^T[ACGT]AGG
CfoI 1080 GCGC GCG^C
Cfr10I 365 ACCGGC [GA]^CCGG[CT]
DrdI 425 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 466 CTCTTC CTCTTC(1/4)
Eco81I 1177 CCTGAGG CC^T[ACGT]AGG
EcoNI 1290 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 827 GAATTC G^AATTC
EcoRV 1525 GATATC GAT^ATC
EcoT14I 870 CCTTGG C^C[AT][AT]GG
EcoT22I 437 ATGCAT ATGCA^T
FbaI 1421 TGATCA T^GATCA
HhaI 1080 GCGC GCG^C
Hin1I 1018 GGCGTC G[GA]^CG[CT]C
HinP1I 1080 GCGC G^CGC
Ksp632I 466 CTCTTC CTCTTC(1/4)
MflI 23 GGATCT [GA]^GATC[CT]
MvnI 1415 CGCG CG^CG
NsiI 437 ATGCAT ATGCA^T
PshBI 690 ATTAAT AT^TAAT
SapI 465 GCTCTTC GCTCTTC(1/4)
SbfI 1031 CCTGCAGG CCTGCA^GG
ScaI 7 AGTACT AGT^ACT
SmaI 128 CCCGGG CCC^GGG
SmlI 568 CTCAAG C^T[CT][GA]AG
Sse8387I 1031 CCTGCAGG CCTGCA^GG
SspBI 1514 TGTACA T^GTACA
StyI 870 CCTTGG C^C[AT][AT]GG
XhoII 23 GGATCT [GA]^GATC[CT]
XmaCI 128 CCCGGG C^CCGGG
XmaI 128 CCCGGG C^CCGGG
XmnI 250 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 511 GAGCAC G[AT]GC[AT]^C
967 GAGCAC
BanI 132 GGTGCC G^G[CT][GA]CC
1286 GGCACC
BanII 1345 GGGCTC G[GA]GC[CT]^C
1384 GGGCTC
BbrPI 838 CACGTG CAC^GTG
917 CACGTG
BbvCI 1197 GCTGAGG CCTCAGC(-5/-2)
1294 GCTGAGG
BlpI 1171 GCTGAGC GC^T[ACGT]AGC
1251 GCTGAGC
Bpu1102I 1171 GCTGAGC GC^T[ACGT]AGC
1251 GCTGAGC
BsaAI 838 CACGTG [CT]AC^GT[GA]
917 CACGTG
BsiHKAI 511 GAGCAC G[AT]GC[AT]^C
967 GAGCAC
BstAPI 639 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1353 GCAGAGCCTGC
CelII 1171 GCTGAGC GC^T[ACGT]AGC
1251 GCTGAGC
FauI 1333 CCCGC CCCGC(4/6)
1382 GCGGG
HgaI 71 GACGC GACGC(5/10)
1019 GCGTC
HphI 759 GGTGA GGTGA(8/7)
853 GGTGA
PleI 302 GAGTC GAGTC(4/5)
976 GAGTC
PmaCI 838 CACGTG CAC^GTG
917 CACGTG
PmlI 838 CACGTG CAC^GTG
917 CACGTG
PstI 1032 CTGCAG CTGCA^G
1366 CTGCAG
PvuII 337 CAGCTG CAG^CTG
1054 CAGCTG
TaqI 258 TCGA T^CGA
419 TCGA
TthHB8I 258 TCGA T^CGA
419 TCGA
XcmI 347 CCAATTCCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
879 CCAAGAGGACAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I