Restriction map for
pF1KSDA1344
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsiEI BsiWI BsiYI BslI BsmAI
BsmBI BsoBI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI CpoI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MspA1I MunI MvnI NaeI
NarI NciI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 1842 TCCGGA T^CCGGA
AcyI 884 GGCGTC G[GA]^CG[CT]C
AlwNI 1130 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 514 GAGCAC G[AT]GC[AT]^C
AvaII 1096 GGTCC G^G[AT]CC
BalI 2097 TGGCCA TGG^CCA
BciVI 1261 GTATCC GTATCC(6/5)
BglII 1458 AGATCT A^GATCT
BmyI 514 GAGCAC G[AGT]GC[ACT]^C
BpmI 2012 CTCCAG CTGGAG(16/14)
BsaHI 884 GGCGTC G[GA]^CG[CT]C
BsaWI 1842 TCCGGA [AT]^CCGG[AT]
BseAI 1842 TCCGGA T^CCGGA
BseRI 2222 CTCCTC GAGGAG(10/8)
BsiHKAI 514 GAGCAC G[AT]GC[AT]^C
Bsp1286I 514 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1406 TGTACA T^GTACA
BspDI 1512 ATCGAT AT^CGAT
BspEI 1842 TCCGGA T^CCGGA
BspMI 2202 ACCTGC ACCTGC(4/8)
BsrFI 535 GCCGGT [GA]^CCGG[CT]
BsrGI 1406 TGTACA T^GTACA
Cfr10I 535 GCCGGT [GA]^CCGG[CT]
Cfr13I 1096 GGTCC G^G[ACGT]CC
ClaI 1512 ATCGAT AT^CGAT
DraI 2416 TTTAAA TTT^AAA
DrdI 1592 GACTATTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1265 CCATGG C^C[GA][CT]GG
EaeI 2097 TGGCCA [CT]^GGCC[GA]
EcoRI 337 GAATTC G^AATTC
FauI 2034 GCGGG CCCGC(4/6)
HaeIII 2098 GGCC GG^CC
HgaI 885 GCGTC GACGC(5/10)
Hin1I 884 GGCGTC G[GA]^CG[CT]C
MaeIII 1381 GTTAC ^GT[ACGT]AC
MluNI 2097 TGGCCA TGG^CCA
MroI 1842 TCCGGA T^CCGGA
MscI 2097 TGGCCA TGG^CCA
NcoI 1265 CCATGG C^CATGG
NdeI 488 CATATG CA^TATG
NheI 1783 GCTAGC G^CTAGC
NlaIV 1450 GGAACC GG[ACGT]^[ACGT]CC
SapI 394 GCTCTTC GCTCTTC(1/4)
Sau96I 1096 GGTCC G^G[ACGT]CC
ScaI 1233 AGTACT AGT^ACT
SpeI 651 ACTAGT A^CTAGT
SspBI 1406 TGTACA T^GTACA
TaqI 1513 TCGA T^CGA
TthHB8I 1513 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 2261 CCGC CCGC(-3/-1)
2034 GCGG
BbvI 1717 GCTGC GCAGC(8/12)
1737 GCTGC
BsaJI 239 CCAAGG C^C[ACGT][ACGT]GG
1265 CCATGG
BsgI 1346 GTGCAG GTGCAG(16/14)
2317 GTGCAG
BsmI 366 GAATGC GAATGC(1/-1)
2088 GCATTC
BsrDI 116 CATTGC GCAATG(2/0)
672 GCAATG
EcoT14I 239 CCAAGG C^C[AT][AT]GG
1265 CCATGG
HincII 753 GTTGAC GT[CT]^[GA]AC
1704 GTCAAC
HindII 753 GTTGAC GT[CT]^[GA]AC
1704 GTCAAC
HphI 2079 GGTGA GGTGA(8/7)
720 TCACC
MaeII 2274 ACGT A^CGT
2442 ACGT
MslI 1398 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2089 CATTCAGGTG
MwoI 133 GCCTCTTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
370 GCCATTGTCGC
NspI 62 ACATGC [GA]CATG^[CT]
1791 ACATGC
PleI 925 GACTC GAGTC(4/5)
93 GAGTC
PstI 214 CTGCAG CTGCA^G
866 CTGCAG
SfcI 214 CTGCAG C^T[GA][CT]AG
866 CTGCAG
SmlI 947 CTCAAG C^T[CT][GA]AG
2207 CTCAAG
StyI 239 CCAAGG C^C[AT][AT]GG
1265 CCATGG
TseI 1717 GCTGC G^C[AT]GC
1737 GCTGC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbsI BfaI BpuAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I DdeI DpnII EarI EcoRII
Fnu4HI FokI HapII HinfI HpaII
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MspI MvaI
NdeII NlaIII RsaI Sau3AI ScrFI
SfaNI SspI TfiI Tru9I Tsp509I
TspRI XhoII XmnI