Restriction map for
pF1KSDA1318
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Aor51HI ApaI AscI
Asp718I AviII AvrII BamHI BanI
BbeI BbrPI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiEI BsiWI BsmBI BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoT22I FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MluI
MvnI NaeI NarI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SspI
StuI SwaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 3282 TCCGGA T^CCGGA
AcsI 2238 GAATTC [GA]^AATT[CT]
AflIII 2648 ACATGT A^C[GA][CT]GT
AhdI 214 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2652 GTGCAC G^TGCAC
ApaLI 2652 GTGCAC G^TGCAC
ApoI 2238 GAATTC [GA]^AATT[CT]
Asp700I 4102 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 214 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2763 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3473 CCCGGG C^[CT]CG[GA]G
BbvCI 2750 CCTCAGC CCTCAGC(-5/-2)
BcgI 3116 CGATGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4113 TGATCA T^GATCA
BsaWI 3282 TCCGGA [AT]^CCGG[AT]
BseAI 3282 TCCGGA T^CCGGA
BsmI 126 GCATTC GAATGC(1/-1)
BsoBI 3473 CCCGGG C^[CT]CG[GA]G
BspDI 3114 ATCGAT AT^CGAT
BspEI 3282 TCCGGA T^CCGGA
BspHI 4110 TCATGA T^CATGA
BspLU11I 2648 ACATGT A^CATGT
BsrBI 889 CCGCTC CCGCTC(-3/-3)
CfoI 3049 GCGC GCG^C
ClaI 3114 ATCGAT AT^CGAT
DraIII 3918 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 214 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2238 GAATTC G^AATTC
FauI 888 CCCGC CCCGC(4/6)
FbaI 4113 TGATCA T^GATCA
HaeII 3048 AGCGCC [GA]GCGC^[CT]
HhaI 3049 GCGC GCG^C
HinP1I 3049 GCGC G^CGC
HpaI 1398 GTTAAC GTT^AAC
MfeI 1579 CAATTG C^AATTG
MroI 3282 TCCGGA T^CCGGA
MunI 1579 CAATTG C^AATTG
NdeI 2803 CATATG CA^TATG
NheI 585 GCTAGC G^CTAGC
PflFI 2763 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4110 TCATGA T^CATGA
SapI 3457 GAAGAGC GCTCTTC(1/4)
SexAI 3814 ACCAGGT A^CC[AT]GGT
SmaI 3473 CCCGGG CCC^GGG
SpeI 2700 ACTAGT A^CTAGT
TaqI 3115 TCGA T^CGA
Tth111I 2763 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3115 TCGA T^CGA
XcmI 1235 CCACACCGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3473 CCCGGG C^CCGGG
XmaI 3473 CCCGGG C^CCGGG
XmnI 4102 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AseI 579 ATTAAT AT^TAAT
2856 ATTAAT
AsnI 579 ATTAAT AT^TAAT
2856 ATTAAT
BalI 1406 TGGCCA TGG^CCA
3041 TGGCCA
BbsI 709 GAAGAC GAAGAC(2/6)
4106 GTCTTC
BpuAI 709 GAAGAC GAAGAC(2/6)
4106 GTCTTC
EaeI 1406 TGGCCA [CT]^GGCC[GA]
3041 TGGCCA
EcoRV 489 GATATC GAT^ATC
762 GATATC
MaeII 113 ACGT A^CGT
2962 ACGT
MluNI 1406 TGGCCA TGG^CCA
3041 TGGCCA
MscI 1406 TGGCCA TGG^CCA
3041 TGGCCA
NspI 2648 ACATGT [GA]CATG^[CT]
4053 GCATGT
PshAI 2801 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3782 GACATTTGTC
PshBI 579 ATTAAT AT^TAAT
2856 ATTAAT
PstI 482 CTGCAG CTGCA^G
1688 CTGCAG
PvuII 821 CAGCTG CAG^CTG
3928 CAGCTG
SmlI 2096 CTCAAG C^T[CT][GA]AG
3960 CTCAAG
Tsp45I 2608 GTGAC ^GT[GC]AC
3009 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BanII
BbvI BcnI BfaI BglII BmyI
BpmI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TfiI Tru9I
TseI Tsp509I TspRI Van91I XbaI
XhoII