Restriction map for
pF1KSDA1317
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspHI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MluI MluNI MscI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 1097 TCCGGA T^CCGGA
AhdI 946 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1018 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 70 CCTGAGG CC^T[ACGT]AGG
ApaI 1044 GGGCCC GGGCC^C
Asp700I 587 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 946 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 349 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 35 CTCGAG C^[CT]CG[GA]G
BamHI 514 GGATCC G^GATCC
BciVI 1193 GTATCC GTATCC(6/5)
BclI 291 TGATCA T^GATCA
BcnI 569 CCCGG CC^[GC]GG
BglII 797 AGATCT A^GATCT
BsaBI 129 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 638 GAGACC GGTCTC(1/5)
BsaWI 1097 TCCGGA [AT]^CCGG[AT]
BseAI 1097 TCCGGA T^CCGGA
BsgI 519 CTGCAC GTGCAG(16/14)
BsiEI 477 CGACCG CG[GA][CT]^CG
BsmBI 976 GAGACG CGTCTC(1/5)
BsoBI 35 CTCGAG C^[CT]CG[GA]G
BspEI 1097 TCCGGA T^CCGGA
BspMI 276 GCAGGT ACCTGC(4/8)
BsrBI 906 GAGCGG CCGCTC(-3/-3)
BsrDI 920 GCAATG GCAATG(2/0)
BsrI 951 CCAGT ACTGG(1/-1)
BstAPI 911 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 848 GGTCACC G^GT[ACGT]ACC
BstPI 848 GGTCACC G^GT[ACGT]ACC
Bsu36I 70 CCTGAGG CC^T[ACGT]AGG
Cac8I 961 GCCAGC GC[ACGT]^[ACGT]GC
DraIII 736 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 946 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 70 CCTGAGG CC^T[ACGT]AGG
EcoO65I 848 GGTCACC G^GT[ACGT]ACC
EcoRI 397 GAATTC G^AATTC
FbaI 291 TGATCA T^GATCA
MaeII 913 ACGT A^CGT
MamI 129 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1169 CAATTG C^AATTG
MroI 1097 TCCGGA T^CCGGA
MslI 495 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1054 CAGCTG C[AC]G^C[GT]G
MunI 1169 CAATTG C^AATTG
NciI 569 CCCGG CC^[GC]GG
PaeR7I 35 CTCGAG C^TCGAG
PflFI 349 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1054 CAGCTG CAG^CTG
SfcI 18 CTGTAG C^T[GA][CT]AG
SmlI 35 CTCGAG C^T[CT][GA]AG
Tth111I 349 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 35 CTCGAG C^TCGAG
XmnI 587 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanII 650 GAGCCC G[GA]GC[CT]^C
1044 GGGCCC
BbsI 311 GAAGAC GAAGAC(2/6)
823 GTCTTC
BfaI 193 CTAG C^TAG
938 CTAG
BmyI 650 GAGCCC G[AGT]GC[ACT]^C
1044 GGGCCC
BpuAI 311 GAAGAC GAAGAC(2/6)
823 GTCTTC
BseRI 1174 GAGGAG GAGGAG(10/8)
1177 GAGGAG
Bsp1286I 650 GAGCCC G[AGT]GC[ACT]^C
1044 GGGCCC
BstYI 514 GGATCC [GA]^GATC[CT]
797 AGATCT
DraII 1043 AGGGCCC [GA]G^G[ACGT]CC[CT]
1044 GGGCCCT
EcoO109I 1043 AGGGCCC [GA]G^G[ACGT]CC[CT]
1044 GGGCCCT
HapII 570 CCGG C^CGG
1098 CCGG
HpaII 570 CCGG C^CGG
1098 CCGG
MaeI 193 CTAG C^TAG
938 CTAG
MflI 514 GGATCC [GA]^GATC[CT]
797 AGATCT
MseI 1154 TTAA T^TAA
1267 TTAA
MspI 570 CCGG C^CGG
1098 CCGG
NlaIII 738 CATG CATG^
1131 CATG
PflMI 735 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCAGACTCTGG
Tru9I 1154 TTAA T^TAA
1267 TTAA
Van91I 735 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1017 CCAGACTCTGG
XhoII 514 GGATCC [GA]^GATC[CT]
797 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I