Restriction map for
pF1KSDA1284
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AvrII
BalI BamHI BanI BbeI BcgI
BcnI BglII BlnI BsaBI BsaHI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI Bsu36I Cfr10I
ClaI CpoI DrdI DsaI EaeI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV FauI FseI Hin1I
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MslI MunI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 26 CGCG CG^CG
AccIII 1746 TCCGGA T^CCGGA
AflII 2266 CTTAAG C^TTAAG
AhdI 2373 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2591 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 377 AGCGCT AGC^GCT
AspEI 2373 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 111 CTCGGG C^[CT]CG[GA]G
AviII 1828 TGCGCA TGC^GCA
BbrPI 1991 CACGTG CAC^GTG
BfrI 2266 CTTAAG C^TTAAG
BlpI 1366 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1366 GCTCAGC GC^T[ACGT]AGC
BsaAI 1991 CACGTG [CT]AC^GT[GA]
BseAI 1746 TCCGGA T^CCGGA
BseRI 914 CTCCTC GAGGAG(10/8)
BsoBI 111 CTCGGG C^[CT]CG[GA]G
BspEI 1746 TCCGGA T^CCGGA
BspHI 2763 TCATGA T^CATGA
BspMI 765 GCAGGT ACCTGC(4/8)
BsrBI 663 GAGCGG CCGCTC(-3/-3)
BsrDI 398 GCAATG GCAATG(2/0)
BstAPI 1486 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 26 CGCG CG^CG
CelII 1366 GCTCAGC GC^T[ACGT]AGC
DraIII 1046 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2373 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 377 AGCGCT AGC^GCT
EcoNI 2448 CCTTGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1592 CCAAGG C^C[AT][AT]GG
EcoT22I 788 ATGCAT ATGCA^T
FspI 1828 TGCGCA TGC^GCA
HgaI 27 GCGTC GACGC(5/10)
HincII 2817 GTTAAC GT[CT]^[GA]AC
HindII 2817 GTTAAC GT[CT]^[GA]AC
HpaI 2817 GTTAAC GTT^AAC
MroI 1746 TCCGGA T^CCGGA
MvnI 26 CGCG CG^CG
NheI 1935 GCTAGC G^CTAGC
NsiI 788 ATGCAT ATGCA^T
PmaCI 1991 CACGTG CAC^GTG
PmlI 1991 CACGTG CAC^GTG
PshAI 447 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1484 CTGCAG CTGCA^G
PvuII 1900 CAGCTG CAG^CTG
RcaI 2763 TCATGA T^CATGA
ScaI 1392 AGTACT AGT^ACT
SpeI 1087 ACTAGT A^CTAGT
StyI 1592 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspI 834 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACTCAGTC
BciVI 415 GTATCC GTATCC(6/5)
890 GTATCC
BclI 1676 TGATCA T^GATCA
2673 TGATCA
BglI 4 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
156 GCCTGAATGGC
BsaI 53 GAGACC GGTCTC(1/5)
2582 GAGACC
BsiYI 1713 CCGACCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2448 CCTTGAAGAGG
BslI 1713 CCGACCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2448 CCTTGAAGAGG
BsmI 749 GCATTC GAATGC(1/-1)
1344 GCATTC
Bst1107I 1533 GTATAC GTA^TAC
1618 GTATAC
BstXI 894 CCAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1899 CCAGCTGGATGG
BstYI 649 GGATCT [GA]^GATC[CT]
2143 GGATCT
BstZ17I 1533 GTATAC GTA^TAC
1618 GTATAC
DraI 1104 TTTAAA TTT^AAA
2800 TTTAAA
DraII 2257 GGGACCT [GA]G^G[ACGT]CC[CT]
2327 AGGACCT
EcoO109I 2257 GGGACCT [GA]G^G[ACGT]CC[CT]
2327 AGGACCT
FbaI 1676 TGATCA T^GATCA
2673 TGATCA
HaeII 377 AGCGCT [GA]GCGC^[CT]
1351 AGCGCC
HindIII 538 AAGCTT A^AGCTT
862 AAGCTT
MflI 649 GGATCT [GA]^GATC[CT]
2143 GGATCT
MspA1I 466 CAGCGG C[AC]G^C[GT]G
1900 CAGCTG
NdeI 791 CATATG CA^TATG
1680 CATATG
NspI 1055 ACATGC [GA]CATG^[CT]
2071 ACATGC
PflFI 834 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACTCAGTC
PpuMI 2257 GGGACCT [GA]G^G[AT]CC[CT]
2327 AGGACCT
SacI 33 GAGCTC GAGCT^C
43 GAGCTC
SapI 660 GAAGAGC GCTCTTC(1/4)
967 GAAGAGC
Tth111I 834 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2767 GACTCAGTC
XhoII 649 GGATCT [GA]^GATC[CT]
2143 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbsI BbvCI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BsgI BsiHKAI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI