Restriction map for
pF1KSDA1281
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# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflII Alw44I ApaLI
AscI Asp700I AspI AviII AvrII
BbsI BcgI BciVI BfrI BglI
BlnI BpuAI BsaBI BsiEI Bsp1407I
BspDI BspHI BsrGI BssHII BssSI
BstBI ClaI CpoI DraIII DrdI
EagI EclXI Eco52I FseI FspI
HindIII MamI MfeI MluI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NspV PacI PflFI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1712 GGTACC G^GTACC
AccIII 3477 TCCGGA T^CCGGA
AflIII 1060 ACATGT A^C[GA][CT]GT
AgeI 1014 ACCGGT A^CCGGT
AlwNI 3334 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1707 CCTGAGG CC^T[ACGT]AGG
Aor51HI 453 AGCGCT AGC^GCT
AseI 2112 ATTAAT AT^TAAT
AsnI 2112 ATTAAT AT^TAAT
Asp718I 1712 GGTACC G^GTACC
BamHI 36 GGATCC G^GATCC
BbrPI 1182 CACGTG CAC^GTG
BclI 3197 TGATCA T^GATCA
BglII 1781 AGATCT A^GATCT
BsaI 298 GAGACC GGTCTC(1/5)
BseAI 3477 TCCGGA T^CCGGA
BsiWI 1199 CGTACG C^GTACG
BsmBI 2476 GAGACG CGTCTC(1/5)
BspEI 3477 TCCGGA T^CCGGA
BspLU11I 1060 ACATGT A^CATGT
BsrBI 4318 GAGCGG CCGCTC(-3/-3)
Bst1107I 3736 GTATAC GTA^TAC
BstEII 4411 GGTTACC G^GT[ACGT]ACC
BstPI 4411 GGTTACC G^GT[ACGT]ACC
BstZ17I 3736 GTATAC GTA^TAC
Bsu36I 1707 CCTGAGG CC^T[ACGT]AGG
DsaI 533 CCGCGG C^C[GA][CT]GG
Eco47III 453 AGCGCT AGC^GCT
Eco81I 1707 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4411 GGTTACC G^GT[ACGT]ACC
EcoRV 1465 GATATC GAT^ATC
EcoT22I 3248 ATGCAT ATGCA^T
FbaI 3197 TGATCA T^GATCA
HpaI 3861 GTTAAC GTT^AAC
KpnI 1712 GGTACC GGTAC^C
KspI 533 CCGCGG CCGC^GG
MroI 3477 TCCGGA T^CCGGA
NdeI 3251 CATATG CA^TATG
NsiI 3248 ATGCAT ATGCA^T
PflMI 3333 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1014 ACCGGT A^CCGGT
PmaCI 1182 CACGTG CAC^GTG
PmlI 1182 CACGTG CAC^GTG
PpuMI 1903 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2112 ATTAAT AT^TAAT
SacI 3394 GAGCTC GAGCT^C
SacII 533 CCGCGG CCGC^GG
SapI 1640 GCTCTTC GCTCTTC(1/4)
SpeI 169 ACTAGT A^CTAGT
SspI 1003 AATATT AAT^ATT
StuI 266 AGGCCT AGG^CCT
Van91I 3333 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3679 GTAGAC GT^[AC][GT]AC
3736 GTATAC
AccII 534 CGCG CG^CG
738 CGCG
AcyI 422 GGCGCC G[GA]^CG[CT]C
598 GGCGCC
AhdI 2854 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3268 GACCAGAAGTC
ApaI 1099 GGGCCC GGGCC^C
1238 GGGCCC
AspEI 2854 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3268 GACCAGAAGTC
AspHI 3069 GAGCAC G[AT]GC[AT]^C
3394 GAGCTC
BbeI 422 GGCGCC GGCGC^C
598 GGCGCC
BlpI 3598 GCTAAGC GC^T[ACGT]AGC
4315 GCTGAGC
Bpu1102I 3598 GCTAAGC GC^T[ACGT]AGC
4315 GCTGAGC
BsaAI 1182 CACGTG [CT]AC^GT[GA]
1201 TACGTG
BsaHI 422 GGCGCC G[GA]^CG[CT]C
598 GGCGCC
BsaWI 1014 ACCGGT [AT]^CCGG[AT]
3477 TCCGGA
BsgI 1221 CTGCAC GTGCAG(16/14)
4378 CTGCAC
BsiHKAI 3069 GAGCAC G[AT]GC[AT]^C
3394 GAGCTC
BsrFI 1014 ACCGGT [GA]^CCGG[CT]
1872 ACCGGC
BstAPI 642 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1312 GCAAGGTCTGC
BstUI 534 CGCG CG^CG
738 CGCG
BstYI 36 GGATCC [GA]^GATC[CT]
1781 AGATCT
CelII 3598 GCTAAGC GC^T[ACGT]AGC
4315 GCTGAGC
Cfr10I 1014 ACCGGT [GA]^CCGG[CT]
1872 ACCGGC
DraI 2559 TTTAAA TTT^AAA
3697 TTTAAA
Eam1105I 2854 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3268 GACCAGAAGTC
Hin1I 422 GGCGCC G[GA]^CG[CT]C
598 GGCGCC
KasI 422 GGCGCC G^GCGCC
598 GGCGCC
MflI 36 GGATCC [GA]^GATC[CT]
1781 AGATCT
MvnI 534 CGCG CG^CG
738 CGCG
NarI 422 GGCGCC GG^CGCC
598 GGCGCC
PaeR7I 2703 CTCGAG C^TCGAG
3690 CTCGAG
ScaI 3320 AGTACT AGT^ACT
4031 AGTACT
SfoI 422 GGCGCC GGC^GCC
598 GGCGCC
SmaI 1332 CCCGGG CCC^GGG
4425 CCCGGG
SphI 4038 GCATGC GCATG^C
4263 GCATGC
XhoI 2703 CTCGAG C^TCGAG
3690 CTCGAG
XhoII 36 GGATCC [GA]^GATC[CT]
1781 AGATCT
XmaCI 1332 CCCGGG C^CCGGG
4425 CCCGGG
XmaI 1332 CCCGGG C^CCGGG
4425 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI