Restriction map for
pF1KSDA1279
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AcyI AflII AflIII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AvaII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmFI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT14I FbaI
FseI HgaI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MscI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RcaI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 380 GGTACC G^GTACC
AccII 59 CGCG CG^CG
AccIII 81 TCCGGA T^CCGGA
AclI 7 AACGTT AA^CGTT
AgeI 1099 ACCGGT A^CCGGT
ApaI 253 GGGCCC GGGCC^C
Asp718I 380 GGTACC G^GTACC
AviII 374 TGCGCA TGC^GCA
BamHI 1488 GGATCC G^GATCC
BbvCI 232 GCTGAGG CCTCAGC(-5/-2)
BglI 246 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 854 AGATCT A^GATCT
BseAI 81 TCCGGA T^CCGGA
BspEI 81 TCCGGA T^CCGGA
BspMI 377 GCAGGT ACCTGC(4/8)
BsrDI 1470 CATTGC GCAATG(2/0)
BstEII 208 GGTGACC G^GT[ACGT]ACC
BstPI 208 GGTGACC G^GT[ACGT]ACC
BstUI 59 CGCG CG^CG
DraII 252 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 349 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 13 CCGTGG C^C[GA][CT]GG
EcoO109I 252 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 208 GGTGACC G^GT[ACGT]ACC
EcoT22I 1336 ATGCAT ATGCA^T
FspI 374 TGCGCA TGC^GCA
HincII 1405 GTCAAC GT[CT]^[GA]AC
HindII 1405 GTCAAC GT[CT]^[GA]AC
KpnI 380 GGTACC GGTAC^C
MaeII 8 ACGT A^CGT
MroI 81 TCCGGA T^CCGGA
MspA1I 821 CAGCTG C[AC]G^C[GT]G
MvnI 59 CGCG CG^CG
NaeI 228 GCCGGC GCC^GGC
NgoMIV 228 GCCGGC G^CCGGC
NsiI 1336 ATGCAT ATGCA^T
NspI 1434 ACATGC [GA]CATG^[CT]
PinAI 1099 ACCGGT A^CCGGT
Psp1406I 7 AACGTT AA^CGTT
PvuII 821 CAGCTG CAG^CTG
SapI 1554 GCTCTTC GCTCTTC(1/4)
ScaI 1544 AGTACT AGT^ACT
SexAI 350 ACCTGGT A^CC[AT]GGT
XcmI 1685 CCAAGAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 649 GTCTAC GT^[AC][GT]AC
1375 GTAGAC
AlwNI 655 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
807 CAGGCACTG
Asp700I 1066 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1609 GAAGATGTTC
BanI 202 GGTGCC G^G[CT][GA]CC
380 GGTACC
BbsI 868 GAAGAC GAAGAC(2/6)
1814 GTCTTC
BpuAI 868 GAAGAC GAAGAC(2/6)
1814 GTCTTC
BsaI 1152 GAGACC GGTCTC(1/5)
1574 GAGACC
BsaWI 81 TCCGGA [AT]^CCGG[AT]
1099 ACCGGT
BsgI 470 CTGCAC GTGCAG(16/14)
1087 GTGCAG
BsmBI 1654 CGTCTC CGTCTC(1/5)
1323 GAGACG
BsrBI 175 GAGCGG CCGCTC(-3/-3)
269 GAGCGG
FauI 60 GCGGG CCCGC(4/6)
337 GCGGG
PflMI 213 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
654 CCAGCATCTGG
PstI 1124 CTGCAG CTGCA^G
1676 CTGCAG
SmaI 116 CCCGGG CCC^GGG
198 CCCGGG
Van91I 213 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
654 CCAGCATCTGG
XmaCI 116 CCCGGG C^CCGGG
198 CCCGGG
XmaI 116 CCCGGG C^CCGGG
198 CCCGGG
XmnI 1066 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1609 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI BanII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EarI Eco81I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII