Restriction map for
pF1KSDA1277
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# NO CUT ENZYMES ( 87 enzymes) #
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AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AviII
AvrII BalI BciVI BfrI BglII
BlnI BsaBI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HpaI KspI MamI
MfeI MluI MluNI MscI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI PvuI RsrII SacI SacII
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XcmI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 2754 GACGTC GACGT^C
Acc65I 1643 GGTACC G^GTACC
AccI 2419 GTCGAC GT^[AC][GT]AC
AccIII 1478 TCCGGA T^CCGGA
AclI 813 AACGTT AA^CGTT
AflIII 746 ACGTGT A^C[GA][CT]GT
AhdI 2224 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2644 GGGCCC GGGCC^C
Asp718I 1643 GGTACC G^GTACC
AspEI 2224 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2518 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1635 GGCGCC GGCGC^C
BbrPI 2218 CACGTG CAC^GTG
BclI 23 TGATCA T^GATCA
BglI 49 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2322 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2322 GCTCAGC GC^T[ACGT]AGC
BsaI 1406 GAGACC GGTCTC(1/5)
BseAI 1478 TCCGGA T^CCGGA
BspEI 1478 TCCGGA T^CCGGA
BsrBI 2881 GAGCGG CCGCTC(-3/-3)
BsrDI 2310 CATTGC GCAATG(2/0)
CelII 2322 GCTCAGC GC^T[ACGT]AGC
DsaI 2414 CCATGG C^C[GA][CT]GG
Eam1105I 2224 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 147 GATATC GAT^ATC
FbaI 23 TGATCA T^GATCA
HindIII 2578 AAGCTT A^AGCTT
KasI 1635 GGCGCC G^GCGCC
KpnI 1643 GGTACC GGTAC^C
MroI 1478 TCCGGA T^CCGGA
NaeI 2894 GCCGGC GCC^GGC
NarI 1635 GGCGCC GG^CGCC
NcoI 2414 CCATGG C^CATGG
NgoMIV 2894 GCCGGC G^CCGGC
NspI 822 ACATGC [GA]CATG^[CT]
PflFI 2518 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2218 CACGTG CAC^GTG
PmlI 2218 CACGTG CAC^GTG
Psp1406I 813 AACGTT AA^CGTT
PvuII 38 CAGCTG CAG^CTG
SalI 2419 GTCGAC G^TCGAC
SapI 1689 GCTCTTC GCTCTTC(1/4)
ScaI 1697 AGTACT AGT^ACT
SexAI 2261 ACCTGGT A^CC[AT]GGT
SfoI 1635 GGCGCC GGC^GCC
SmaI 1658 CCCGGG CCC^GGG
StuI 2792 AGGCCT AGG^CCT
TfiI 1457 GAATC G^A[AT]TC
Tth111I 2518 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1119 TCTAGA T^CTAGA
XmaCI 1658 CCCGGG C^CCGGG
XmaI 1658 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 1059 CGCG CG^CG
2349 CGCG
AlwNI 1600 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2168 CAGGAACTG
Asp700I 1111 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2596 GAACACATTC
BamHI 405 GGATCC G^GATCC
531 GGATCC
BbvCI 2247 CCTCAGC CCTCAGC(-5/-2)
1810 GCTGAGG
BcgI 1632 GCAGGCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1928 GCAGAAGGTTCG
BsaAI 1744 TACGTG [CT]AC^GT[GA]
2218 CACGTG
BsaWI 731 ACCGGA [AT]^CCGG[AT]
1478 TCCGGA
BsgI 185 CTGCAC GTGCAG(16/14)
983 GTGCAG
BspHI 1799 TCATGA T^CATGA
2093 TCATGA
BspMI 1260 ACCTGC ACCTGC(4/8)
483 GCAGGT
BsrFI 2531 GCCGGT [GA]^CCGG[CT]
2894 GCCGGC
BstUI 1059 CGCG CG^CG
2349 CGCG
Cfr10I 2531 GCCGGT [GA]^CCGG[CT]
2894 GCCGGC
HgaI 2439 GACGC GACGC(5/10)
2252 GCGTC
MseI 1397 TTAA T^TAA
1909 TTAA
MslI 2394 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2965 CACAGCGGTG
MvnI 1059 CGCG CG^CG
2349 CGCG
PaeR7I 496 CTCGAG C^TCGAG
2212 CTCGAG
RcaI 1799 TCATGA T^CATGA
2093 TCATGA
Tru9I 1397 TTAA T^TAA
1909 TTAA
XhoI 496 CTCGAG C^TCGAG
2212 CTCGAG
XmnI 1111 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2596 GAACACATTC
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
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AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII