Restriction map for
pF1KSDA1276
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AvrII BalI BciVI
BclI BglII BlnI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 3063 GTCTAC GT^[AC][GT]AC
AcyI 67 GGCGCC G[GA]^CG[CT]C
AflII 910 CTTAAG C^TTAAG
Alw44I 1854 GTGCAC G^TGCAC
ApaI 567 GGGCCC GGGCC^C
ApaLI 1854 GTGCAC G^TGCAC
AseI 2986 ATTAAT AT^TAAT
AsnI 2986 ATTAAT AT^TAAT
BamHI 1596 GGATCC G^GATCC
BbeI 67 GGCGCC GGCGC^C
BbrPI 103 CACGTG CAC^GTG
BfrI 910 CTTAAG C^TTAAG
BlpI 627 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 627 GCTGAGC GC^T[ACGT]AGC
BsaAI 103 CACGTG [CT]AC^GT[GA]
BsaHI 67 GGCGCC G[GA]^CG[CT]C
BsmBI 382 GAGACG CGTCTC(1/5)
BssHII 2463 GCGCGC G^CGCGC
CelII 627 GCTGAGC GC^T[ACGT]AGC
DraIII 1315 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 67 GGCGCC G[GA]^CG[CT]C
KasI 67 GGCGCC G^GCGCC
NarI 67 GGCGCC GG^CGCC
NcoI 2600 CCATGG C^CATGG
NspI 2003 GCATGC [GA]CATG^[CT]
PaeR7I 1975 CTCGAG C^TCGAG
PmaCI 103 CACGTG CAC^GTG
PmlI 103 CACGTG CAC^GTG
PshBI 2986 ATTAAT AT^TAAT
ScaI 3167 AGTACT AGT^ACT
SfoI 67 GGCGCC GGC^GCC
SphI 2003 GCATGC GCATG^C
StuI 2114 AGGCCT AGG^CCT
XbaI 1779 TCTAGA T^CTAGA
XcmI 443 CCAGGGAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1975 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 806 TCCGGA T^CCGGA
2677 TCCGGA
AfaI 864 GTAC GT^AC
3168 GTAC
Asp700I 283 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1222 GAAGACCTTC
AviII 271 TGCGCA TGC^GCA
2000 TGCGCA
BanI 67 GGCGCC G^G[CT][GA]CC
2237 GGCACC
BcgI 116 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1002 GCAGGAGTGTCG
BseAI 806 TCCGGA T^CCGGA
2677 TCCGGA
BspEI 806 TCCGGA T^CCGGA
2677 TCCGGA
BspMI 1877 ACCTGC ACCTGC(4/8)
2516 GCAGGT
BssSI 502 CACGAG CACGAG(-5/-1)
369 CTCGTG
BstXI 2552 CCAGGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2912 CCAGCCGCGTGG
BstYI 1596 GGATCC [GA]^GATC[CT]
2081 AGATCC
EaeI 2730 TGGCCG [CT]^GGCC[GA]
3039 TGGCCG
EcoRI 930 GAATTC G^AATTC
1473 GAATTC
FspI 271 TGCGCA TGC^GCA
2000 TGCGCA
MaeII 104 ACGT A^CGT
2906 ACGT
MaeIII 851 GTGAC ^GT[ACGT]AC
1721 GTGAC
MflI 1596 GGATCC [GA]^GATC[CT]
2081 AGATCC
MroI 806 TCCGGA T^CCGGA
2677 TCCGGA
PflMI 303 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2112 CCAGGCCTTGG
RsaI 864 GTAC GT^AC
3168 GTAC
SmaI 2358 CCCGGG CCC^GGG
2862 CCCGGG
TaqI 921 TCGA T^CGA
1976 TCGA
Tsp45I 851 GTGAC ^GT[GC]AC
1721 GTGAC
TthHB8I 921 TCGA T^CGA
1976 TCGA
Van91I 303 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2112 CCAGGCCTTGG
XhoII 1596 GGATCC [GA]^GATC[CT]
2081 AGATCC
XmaCI 2358 CCCGGG C^CCGGG
2862 CCCGGG
XmaI 2358 CCCGGG C^CCGGG
2862 CCCGGG
XmnI 283 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1222 GAAGACCTTC
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TfiI Tru9I
TseI Tsp509I TspRI