Restriction map for
pF1KSDA1267
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI BalI
BbrPI BcgI BglII BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EcoNI
EcoO65I EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KspI MluI
MluNI MroI MscI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1452 GGTACC G^GTACC
AccI 1602 GTCTAC GT^[AC][GT]AC
AccII 2626 CGCG CG^CG
AcyI 12 GGCGCC G[GA]^CG[CT]C
AflII 2113 CTTAAG C^TTAAG
AflIII 1777 ACACGT A^C[GA][CT]GT
AocI 3200 CCTCAGG CC^T[ACGT]AGG
Asp718I 1452 GGTACC G^GTACC
AviII 1897 TGCGCA TGC^GCA
AvrII 2965 CCTAGG C^CTAGG
BamHI 171 GGATCC G^GATCC
BbeI 12 GGCGCC GGCGC^C
BbvCI 1699 GCTGAGG CCTCAGC(-5/-2)
BciVI 3048 GGATAC GTATCC(6/5)
BclI 504 TGATCA T^GATCA
BfrI 2113 CTTAAG C^TTAAG
BglI 2661 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2965 CCTAGG C^CTAGG
BpmI 759 CTCCAG CTGGAG(16/14)
BsaAI 1418 TACGTG [CT]AC^GT[GA]
BsaHI 12 GGCGCC G[GA]^CG[CT]C
BsiEI 2856 CGGCCG CG[GA][CT]^CG
BspHI 507 TCATGA T^CATGA
BsrDI 159 CATTGC GCAATG(2/0)
BsrFI 717 GCCGGC [GA]^CCGG[CT]
BssSI 2805 CACGAG CACGAG(-5/-1)
BstUI 2626 CGCG CG^CG
Bsu36I 3200 CCTCAGG CC^T[ACGT]AGG
Cfr10I 717 GCCGGC [GA]^CCGG[CT]
DraIII 1901 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2856 CGGCCG C^GGCCG
EclXI 2856 CGGCCG C^GGCCG
Eco52I 2856 CGGCCG C^GGCCG
Eco81I 3200 CCTCAGG CC^T[ACGT]AGG
EcoRI 1385 GAATTC G^AATTC
FbaI 504 TGATCA T^GATCA
FspI 1897 TGCGCA TGC^GCA
Hin1I 12 GGCGCC G[GA]^CG[CT]C
KasI 12 GGCGCC G^GCGCC
KpnI 1452 GGTACC GGTAC^C
MfeI 618 CAATTG C^AATTG
MunI 618 CAATTG C^AATTG
MvnI 2626 CGCG CG^CG
NaeI 717 GCCGGC GCC^GGC
NarI 12 GGCGCC GG^CGCC
NgoMIV 717 GCCGGC G^CCGGC
PshAI 1292 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 507 TCATGA T^CATGA
SacI 2571 GAGCTC GAGCT^C
SapI 559 GCTCTTC GCTCTTC(1/4)
SexAI 747 ACCTGGT A^CC[AT]GGT
SfoI 12 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1385 GAATTC [GA]^AATT[CT]
1940 AAATTC
Aor51HI 908 AGCGCT AGC^GCT
2306 AGCGCT
ApoI 1385 GAATTC [GA]^AATT[CT]
1940 AAATTC
AvaI 1267 CCCGAG C^[CT]CG[GA]G
3017 CTCGGG
BlpI 364 GCTGAGC GC^T[ACGT]AGC
3295 GCTCAGC
Bpu1102I 364 GCTGAGC GC^T[ACGT]AGC
3295 GCTCAGC
BsaBI 584 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1882 GATGTGAATC
BsaWI 53 TCCGGT [AT]^CCGG[AT]
1853 ACCGGA
BsmBI 1737 CGTCTC CGTCTC(1/5)
1290 GAGACG
BsoBI 1267 CCCGAG C^[CT]CG[GA]G
3017 CTCGGG
BsrBI 17 CCGCTC CCGCTC(-3/-3)
3106 GAGCGG
BstYI 171 GGATCC [GA]^GATC[CT]
583 GGATCT
CelII 364 GCTGAGC GC^T[ACGT]AGC
3295 GCTCAGC
EaeI 2856 CGGCCG [CT]^GGCC[GA]
3216 TGGCCG
Eco47III 908 AGCGCT AGC^GCT
2306 AGCGCT
MamI 584 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1882 GATGTGAATC
MflI 171 GGATCC [GA]^GATC[CT]
583 GGATCT
NspI 2048 GCATGC [GA]CATG^[CT]
2760 GCATGC
PstI 164 CTGCAG CTGCA^G
1316 CTGCAG
SphI 2048 GCATGC GCATG^C
2760 GCATGC
XhoII 171 GGATCC [GA]^GATC[CT]
583 GGATCT
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI