Restriction map for
pF1KSDA1257
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwI AocI ApaLI AscI Asp700I
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbsI BbvCI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII HpaI KasI
KspI MfeI MflI MluI MroI
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 1168 GGTACC G^GTACC
AfaI 1169 GTAC GT^AC
AhdI 586 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1195 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1126 AGCGCT AGC^GCT
ApaI 125 GGGCCC GGGCC^C
AseI 960 ATTAAT AT^TAAT
AsnI 960 ATTAAT AT^TAAT
Asp718I 1168 GGTACC G^GTACC
AspEI 586 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 1168 GGTACC G^G[CT][GA]CC
BbrPI 211 CACGTG CAC^GTG
BcgI 155 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 65 CTAG C^TAG
BglI 1215 GCCAGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 436 CTGGAG CTGGAG(16/14)
BsaAI 211 CACGTG [CT]AC^GT[GA]
BsaBI 945 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1013 GGTCTC GGTCTC(1/5)
BsgI 153 GTGCAG GTGCAG(16/14)
BsrBI 1156 CCGCTC CCGCTC(-3/-3)
BssSI 81 CACGAG CACGAG(-5/-1)
BstAPI 1194 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1127 GCGC GCG^C
DpnII 951 GATC ^GATC
DraI 684 TTTAAA TTT^AAA
DsaI 463 CCGTGG C^C[GA][CT]GG
Eam1105I 586 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1126 AGCGCT AGC^GCT
EcoT14I 116 CCAAGG C^C[AT][AT]GG
FauI 34 CCCGC CCCGC(4/6)
HaeII 1126 AGCGCT [GA]GCGC^[CT]
HgaI 339 GCGTC GACGC(5/10)
HhaI 1127 GCGC GCG^C
HinP1I 1127 GCGC G^CGC
HincII 397 GTTGAC GT[CT]^[GA]AC
HindII 397 GTTGAC GT[CT]^[GA]AC
KpnI 1168 GGTACC GGTAC^C
MaeI 65 CTAG C^TAG
MamI 945 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 951 GATC ^GATC
MslI 469 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1141 CAGCTG C[AC]G^C[GT]G
NaeI 628 GCCGGC GCC^GGC
NdeII 951 GATC ^GATC
NgoMIV 628 GCCGGC G^CCGGC
NspI 9 ACATGC [GA]CATG^[CT]
PmaCI 211 CACGTG CAC^GTG
PmlI 211 CACGTG CAC^GTG
PpuMI 29 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1007 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 960 ATTAAT AT^TAAT
PvuII 1141 CAGCTG CAG^CTG
RsaI 1169 GTAC GT^AC
Sau3AI 951 GATC ^GATC
SfiI 1214 GGCCAGAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 564 CTTGAG C^T[CT][GA]AG
StyI 116 CCAAGG C^C[AT][AT]GG
XcmI 425 CCAAAGTATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 226 AAATTC [GA]^AATT[CT]
546 AAATTT
ApoI 226 AAATTC [GA]^AATT[CT]
546 AAATTT
AvaII 30 GGACC G^G[AT]CC
216 GGTCC
BalI 422 TGGCCA TGG^CCA
1213 TGGCCA
BseRI 54 CTCCTC GAGGAG(10/8)
1059 GAGGAG
BsmFI 217 GTCCC GGGAC(10/14)
864 GTCCC
BsrFI 628 GCCGGC [GA]^CCGG[CT]
1165 GCCGGT
BsrI 240 ACTGG ACTGG(1/-1)
75 CCAGT
BstF5I 757 CATCC GGATG(2/0)
1137 CATCC
Cfr10I 628 GCCGGC [GA]^CCGG[CT]
1165 GCCGGT
DdeI 176 CTGAG C^T[ACGT]AG
979 CTTAG
FokI 757 CATCC GGATG(9/13)
1137 CATCC
MluNI 422 TGGCCA TGG^CCA
1213 TGGCCA
MscI 422 TGGCCA TGG^CCA
1213 TGGCCA
SfaNI 336 GATGC GCATC(5/9)
161 GCATC
TaqI 164 TCGA T^CGA
745 TCGA
TthHB8I 164 TCGA T^CGA
745 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstNI BstXI Cac8I Cfr13I
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MseI MspI MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI