Restriction map for
pF1KSDA1252
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BbeI BbrPI
BbvCI BcgI BclI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI BspDI
BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HaeII HpaI KasI KpnI
KspI MaeII MamI MluI MroI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsrII SacII
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 409 GACGCC G[GA]^CG[CT]C
AflII 388 CTTAAG C^TTAAG
AflIII 305 ACATGT A^C[GA][CT]GT
AlwNI 355 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 1657 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 902 TGGCCA TGG^CCA
BamHI 1360 GGATCC G^GATCC
BanI 1567 GGTGCC G^G[CT][GA]CC
BciVI 988 GGATAC GTATCC(6/5)
BfaI 5 CTAG C^TAG
BfrI 388 CTTAAG C^TTAAG
BsaHI 409 GACGCC G[GA]^CG[CT]C
BseRI 457 GAGGAG GAGGAG(10/8)
BspHI 1532 TCATGA T^CATGA
BspLU11I 305 ACATGT A^CATGT
Bst1107I 61 GTATAC GTA^TAC
BstZ17I 61 GTATAC GTA^TAC
CfoI 1202 GCGC GCG^C
DraI 208 TTTAAA TTT^AAA
DraII 773 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 902 TGGCCA [CT]^GGCC[GA]
EarI 818 GAAGAG CTCTTC(1/4)
EcoNI 477 CCTTGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 773 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 526 GATATC GAT^ATC
EcoT14I 284 CCAAGG C^C[AT][AT]GG
HhaI 1202 GCGC GCG^C
Hin1I 409 GACGCC G[GA]^CG[CT]C
HinP1I 1202 GCGC G^CGC
HindIII 341 AAGCTT A^AGCTT
Ksp632I 818 GAAGAG CTCTTC(1/4)
MaeI 5 CTAG C^TAG
MfeI 1339 CAATTG C^AATTG
MluNI 902 TGGCCA TGG^CCA
MscI 902 TGGCCA TGG^CCA
MspA1I 1043 CAGCTG C[AC]G^C[GT]G
MunI 1339 CAATTG C^AATTG
NspI 305 ACATGT [GA]CATG^[CT]
PflFI 1657 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 773 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 943 GACGCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1043 CAGCTG CAG^CTG
RcaI 1532 TCATGA T^CATGA
SalI 940 GTCGAC G^TCGAC
SapI 818 GAAGAGC GCTCTTC(1/4)
StuI 29 AGGCCT AGG^CCT
StyI 284 CCAAGG C^C[AT][AT]GG
Tth111I 1657 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 61 GTATAC GT^[AC][GT]AC
940 GTCGAC
AciI 771 GCGG CCGC(-3/-1)
807 GCGG
BcnI 1017 CCGGG CC^[GC]GG
1475 CCCGG
BglI 904 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1207 GCCTGTTGGGC
Bsp1407I 446 TGTACA T^GTACA
1646 TGTACA
BsrGI 446 TGTACA T^GTACA
1646 TGTACA
FauI 771 GCGGG CCCGC(4/6)
807 GCGGG
HapII 1017 CCGG C^CGG
1476 CCGG
HgaI 409 GACGC GACGC(5/10)
943 GACGC
HincII 940 GTCGAC GT[CT]^[GA]AC
1597 GTTGAC
HindII 940 GTCGAC GT[CT]^[GA]AC
1597 GTTGAC
HpaII 1017 CCGG C^CGG
1476 CCGG
MspI 1017 CCGG C^CGG
1476 CCGG
NciI 1017 CCGGG CC^[GC]GG
1475 CCCGG
SacI 363 GAGCTC GAGCT^C
472 GAGCTC
SmlI 388 CTTAAG C^T[CT][GA]AG
1288 CTCAAG
SspBI 446 TGTACA T^GTACA
1646 TGTACA
XcmI 187 CCAGAGAGTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1571 CCATCTATGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AfaI AluI AlwI AspHI AvaII
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII