Restriction map for
pF1KSDA1244
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
Alw44I ApaLI ApoI AscI AseI
AsnI Asp718I AviII BciVI BfrI
BglII BsaBI BseAI BsiWI Bsp1407I
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI EcoO65I EcoRI FseI FspI
HincII HindII HpaI KpnI MamI
MluI MroI NdeI NheI NotI
NruI NspV PacI PmeI PshBI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 2800 GACGTC GACGT^C
AhdI 871 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3168 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2131 AGCGCT AGC^GCT
ApaI 3771 GGGCCC GGGCC^C
AspEI 871 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 927 CCTAGG C^CTAGG
BamHI 4977 GGATCC G^GATCC
BlnI 927 CCTAGG C^CTAGG
BsaI 2874 GAGACC GGTCTC(1/5)
BspDI 1603 ATCGAT AT^CGAT
BspLU11I 2655 ACATGT A^CATGT
BsrDI 2479 GCAATG GCAATG(2/0)
BstAPI 339 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1649 CCAAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3168 CCTCAGG CC^T[ACGT]AGG
ClaI 1603 ATCGAT AT^CGAT
DrdI 2809 GACCAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 871 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2131 AGCGCT AGC^GCT
Eco81I 3168 CCTCAGG CC^T[ACGT]AGG
EcoRV 3637 GATATC GAT^ATC
EcoT22I 228 ATGCAT ATGCA^T
KspI 2283 CCGCGG CCGC^GG
MfeI 3384 CAATTG C^AATTG
MunI 3384 CAATTG C^AATTG
NaeI 3244 GCCGGC GCC^GGC
NgoMIV 3244 GCCGGC G^CCGGC
NsiI 228 ATGCAT ATGCA^T
PaeR7I 532 CTCGAG C^TCGAG
SacII 2283 CCGCGG CCGC^GG
SapI 904 GAAGAGC GCTCTTC(1/4)
SphI 226 GCATGC GCATG^C
SspI 3222 AATATT AAT^ATT
XhoI 532 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 655 GTAGAC GT^[AC][GT]AC
724 GTAGAC
AgeI 935 ACCGGT A^CCGGT
3328 ACCGGT
Asp700I 1281 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAAGCTTTTC
AspI 735 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2242 GACCTCGTC
BbeI 1266 GGCGCC GGCGC^C
4390 GGCGCC
BcgI 4226 CGATACAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1721 GCATGAGCCTCG
BclI 819 TGATCA T^GATCA
3656 TGATCA
BsaWI 935 ACCGGT [AT]^CCGG[AT]
3328 ACCGGT
BsiEI 1255 CGGCCG CG[GA][CT]^CG
4484 CGGCCG
BspHI 1289 TCATGA T^CATGA
2297 TCATGA
EagI 1255 CGGCCG C^GGCCG
4484 CGGCCG
EclXI 1255 CGGCCG C^GGCCG
4484 CGGCCG
Eco52I 1255 CGGCCG C^GGCCG
4484 CGGCCG
EcoNI 2625 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4506 CCTTTTTGAGG
FbaI 819 TGATCA T^GATCA
3656 TGATCA
KasI 1266 GGCGCC G^GCGCC
4390 GGCGCC
MseI 2495 TTAA T^TAA
4427 TTAA
NarI 1266 GGCGCC GG^CGCC
4390 GGCGCC
PflFI 735 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2242 GACCTCGTC
PinAI 935 ACCGGT A^CCGGT
3328 ACCGGT
PstI 729 CTGCAG CTGCA^G
1084 CTGCAG
RcaI 1289 TCATGA T^CATGA
2297 TCATGA
ScaI 824 AGTACT AGT^ACT
3956 AGTACT
SfoI 1266 GGCGCC GGC^GCC
4390 GGCGCC
Tru9I 2495 TTAA T^TAA
4427 TTAA
Tth111I 735 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2242 GACCTCGTC
XmnI 1281 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2992 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PshAI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI