Restriction map for
pF1KSDA1227
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BbeI
BbvCI BcgI BciVI BlnI BsaBI
BsaHI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI DraIII
Eco47III EcoO65I EcoRI EcoRV FseI
HaeII Hin1I KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI Tth111I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 1654 CTTAAG C^TTAAG
AhdI 1773 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1773 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2818 CACGTG CAC^GTG
BclI 1904 TGATCA T^GATCA
BcnI 521 CCCGG CC^[GC]GG
BfrI 1654 CTTAAG C^TTAAG
BglI 3008 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2185 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2185 GCTAAGC GC^T[ACGT]AGC
BsaAI 2818 CACGTG [CT]AC^GT[GA]
BsgI 29 CTGCAC GTGCAG(16/14)
BsiEI 2240 CGGCCG CG[GA][CT]^CG
BsmBI 70 CGTCTC CGTCTC(1/5)
BsmI 663 GCATTC GAATGC(1/-1)
BsrBI 2221 GAGCGG CCGCTC(-3/-3)
BstAPI 1643 GCAAACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2185 GCTAAGC GC^T[ACGT]AGC
CpoI 1463 CGGACCG CG^G[AT]CCG
DrdI 2852 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 823 CCACGG C^C[GA][CT]GG
EagI 2240 CGGCCG C^GGCCG
Eam1105I 1773 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2240 CGGCCG C^GGCCG
Eco52I 2240 CGGCCG C^GGCCG
EcoNI 57 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2091 CCTTGG C^C[AT][AT]GG
EcoT22I 2071 ATGCAT ATGCA^T
FbaI 1904 TGATCA T^GATCA
HindIII 2831 AAGCTT A^AGCTT
NciI 521 CCCGG CC^[GC]GG
NsiI 2071 ATGCAT ATGCA^T
PmaCI 2818 CACGTG CAC^GTG
PmlI 2818 CACGTG CAC^GTG
PstI 2256 CTGCAG CTGCA^G
RsrII 1463 CGGACCG CG^G[AT]CCG
SacI 125 GAGCTC GAGCT^C
SapI 285 GAAGAGC GCTCTTC(1/4)
StyI 2091 CCTTGG C^C[AT][AT]GG
SwaI 1635 ATTTAAAT ATTT^AAAT
XbaI 801 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 1218 CCTCAGG CC^T[ACGT]AGG
2262 CCTCAGG
AviII 1280 TGCGCA TGC^GCA
2342 TGCGCA
BglII 1658 AGATCT A^GATCT
1987 AGATCT
BspMI 2326 ACCTGC ACCTGC(4/8)
1675 GCAGGT
BsrDI 537 CATTGC GCAATG(2/0)
1648 CATTGC
BssSI 2218 CACGAG CACGAG(-5/-1)
2296 CACGAG
Bsu36I 1218 CCTCAGG CC^T[ACGT]AGG
2262 CCTCAGG
Eco81I 1218 CCTCAGG CC^T[ACGT]AGG
2262 CCTCAGG
FauI 1305 CCCGC CCCGC(4/6)
2698 GCGGG
FspI 1280 TGCGCA TGC^GCA
2342 TGCGCA
HapII 522 CCGG C^CGG
2232 CCGG
HpaI 1693 GTTAAC GTT^AAC
2560 GTTAAC
HpaII 522 CCGG C^CGG
2232 CCGG
MslI 1684 CACCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2446 CACAATGGTG
MspI 522 CCGG C^CGG
2232 CCGG
PaeR7I 1991 CTCGAG C^TCGAG
2332 CTCGAG
PpuMI 1222 AGGTCCT [GA]G^G[AT]CC[CT]
2700 GGGTCCT
PshAI 1732 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2599 GACCTTAGTC
SexAI 765 ACCAGGT A^CC[AT]GGT
2378 ACCAGGT
SfcI 318 CTATAG C^T[GA][CT]AG
2256 CTGCAG
SphI 2124 GCATGC GCATG^C
2339 GCATGC
StuI 1192 AGGCCT AGG^CCT
3015 AGGCCT
XhoI 1991 CTCGAG C^TCGAG
2332 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI