Restriction map for
pF1KSDA1217
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# NO CUT ENZYMES ( 90 enzymes) #
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Acc65I AclI AflII AgeI Alw44I
AlwNI ApaLI AscI AseI AsnI
Asp700I Asp718I AvaI AvrII BbeI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsiWI BsmI
BsoBI BssHII Bst1107I BstAPI BstBI
BstZ17I CelII CpoI DrdI EagI
EclXI Eco52I EcoNI EcoRV FbaI
FseI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 1134 TCCGGA T^CCGGA
AflIII 2217 ACATGT A^C[GA][CT]GT
AhdI 1865 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1200 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1336 AGCGCT AGC^GCT
AspEI 1865 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 163 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2498 TGGCCA TGG^CCA
BbrPI 2494 CACGTG CAC^GTG
BcgI 3197 CGAGCAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 304 AGATCT A^GATCT
BsaAI 2494 CACGTG [CT]AC^GT[GA]
BseAI 1134 TCCGGA T^CCGGA
BsiEI 167 CGGTCG CG[GA][CT]^CG
BspDI 3462 ATCGAT AT^CGAT
BspEI 1134 TCCGGA T^CCGGA
BspLU11I 2217 ACATGT A^CATGT
BsrBI 765 CCGCTC CCGCTC(-3/-3)
BsrFI 2977 ACCGGC [GA]^CCGG[CT]
Bsu36I 1200 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2977 ACCGGC [GA]^CCGG[CT]
ClaI 3462 ATCGAT AT^CGAT
Eam1105I 1865 GACAGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1336 AGCGCT AGC^GCT
Eco81I 1200 CCTGAGG CC^T[ACGT]AGG
EcoRI 746 GAATTC G^AATTC
EcoT22I 1090 ATGCAT ATGCA^T
HincII 282 GTCAAC GT[CT]^[GA]AC
HindII 282 GTCAAC GT[CT]^[GA]AC
MluNI 2498 TGGCCA TGG^CCA
MroI 1134 TCCGGA T^CCGGA
MscI 2498 TGGCCA TGG^CCA
NdeI 1093 CATATG CA^TATG
NsiI 1090 ATGCAT ATGCA^T
PflFI 163 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2494 CACGTG CAC^GTG
PmlI 2494 CACGTG CAC^GTG
PshAI 2190 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1759 CAGCTG CAG^CTG
SacI 2424 GAGCTC GAGCT^C
SmlI 1699 CTTGAG C^T[CT][GA]AG
StuI 1286 AGGCCT AGG^CCT
Tth111I 163 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 186 GACGTC GACGT^C
2194 GACGTC
AccII 1870 CGCG CG^CG
2418 CGCG
AcyI 186 GACGTC G[GA]^CG[CT]C
2194 GACGTC
AviII 2108 TGCGCA TGC^GCA
2180 TGCGCA
BbvCI 1676 CCTCAGC CCTCAGC(-5/-2)
1779 GCTGAGG
BsaHI 186 GACGTC G[GA]^CG[CT]C
2194 GACGTC
BsaI 107 GAGACC GGTCTC(1/5)
1432 GAGACC
BsaWI 1046 TCCGGT [AT]^CCGG[AT]
1134 TCCGGA
BsmBI 3672 CGTCTC CGTCTC(1/5)
184 GAGACG
BspHI 1905 TCATGA T^CATGA
2753 TCATGA
BstUI 1870 CGCG CG^CG
2418 CGCG
BstXI 1602 CCATGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1985 CCAGCCGATTGG
DraI 1291 TTTAAA TTT^AAA
1504 TTTAAA
DraIII 2455 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2620 CACAGTGTG
FspI 2108 TGCGCA TGC^GCA
2180 TGCGCA
HaeII 853 AGCGCC [GA]GCGC^[CT]
1336 AGCGCT
Hin1I 186 GACGTC G[GA]^CG[CT]C
2194 GACGTC
MvnI 1870 CGCG CG^CG
2418 CGCG
RcaI 1905 TCATGA T^CATGA
2753 TCATGA
XcmI 428 CCATGAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1602 CCATGTGTCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
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AccI AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BssSI BstEII BstF5I BstNI BstPI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII