Restriction map for
pF1KSDA1206
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BfrI BglII BlnI
BsaBI BsiWI BsmBI BspDI BspLU11I
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
MamI MfeI MunI NdeI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 4675 GTCTAC GT^[AC][GT]AC
AflIII 2109 ACGCGT A^C[GA][CT]GT
AgeI 2530 ACCGGT A^CCGGT
AhdI 5253 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4230 AGCGCT AGC^GCT
AspEI 5253 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3630 TGCGCA TGC^GCA
BamHI 3980 GGATCC G^GATCC
BbeI 428 GGCGCC GGCGC^C
BbrPI 1729 CACGTG CAC^GTG
BclI 5619 TGATCA T^GATCA
BsmI 5283 GAATGC GAATGC(1/-1)
BssHII 4014 GCGCGC G^CGCGC
Eam1105I 5253 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4230 AGCGCT AGC^GCT
FbaI 5619 TGATCA T^GATCA
FspI 3630 TGCGCA TGC^GCA
HincII 3298 GTCAAC GT[CT]^[GA]AC
HindII 3298 GTCAAC GT[CT]^[GA]AC
KasI 428 GGCGCC G^GCGCC
MluI 2109 ACGCGT A^CGCGT
NarI 428 GGCGCC GG^CGCC
PaeR7I 247 CTCGAG C^TCGAG
PinAI 2530 ACCGGT A^CCGGT
PmaCI 1729 CACGTG CAC^GTG
PmlI 1729 CACGTG CAC^GTG
SfoI 428 GGCGCC GGC^GCC
SphI 4713 GCATGC GCATG^C
XhoI 247 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 2922 TCCGGA T^CCGGA
5064 TCCGGA
Alw44I 3562 GTGCAC G^TGCAC
4303 GTGCAC
ApaLI 3562 GTGCAC G^TGCAC
4303 GTGCAC
BciVI 1403 GGATAC GTATCC(6/5)
3251 GTATCC
BseAI 2922 TCCGGA T^CCGGA
5064 TCCGGA
BspEI 2922 TCCGGA T^CCGGA
5064 TCCGGA
BspHI 3935 TCATGA T^CATGA
4265 TCATGA
BsrBI 723 CCGCTC CCGCTC(-3/-3)
777 CCGCTC
BsrDI 1134 CATTGC GCAATG(2/0)
3689 GCAATG
BstEII 4282 GGTGACC G^GT[ACGT]ACC
4750 GGTCACC
BstPI 4282 GGTGACC G^GT[ACGT]ACC
4750 GGTCACC
BstYI 3980 GGATCC [GA]^GATC[CT]
5444 AGATCC
EcoO65I 4282 GGTGACC G^GT[ACGT]ACC
4750 GGTCACC
MflI 3980 GGATCC [GA]^GATC[CT]
5444 AGATCC
MroI 2922 TCCGGA T^CCGGA
5064 TCCGGA
MseI 1769 TTAA T^TAA
2729 TTAA
NaeI 2193 GCCGGC GCC^GGC
2624 GCCGGC
NgoMIV 2193 GCCGGC G^CCGGC
2624 GCCGGC
NheI 4227 GCTAGC G^CTAGC
5202 GCTAGC
NspI 4424 ACATGC [GA]CATG^[CT]
4713 GCATGC
RcaI 3935 TCATGA T^CATGA
4265 TCATGA
SapI 4140 GCTCTTC GCTCTTC(1/4)
4428 GCTCTTC
Tru9I 1769 TTAA T^TAA
2729 TTAA
XhoII 3980 GGATCC [GA]^GATC[CT]
5444 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI SspBI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XmaCI
XmaI