Restriction map for
pF1KSDA1205
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccI AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AviII AvrII BcgI BclI
BfaI BglII BlnI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
MaeI MamI MfeI MluI MslI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI ScaI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI TfiI XbaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 2459 GGTACC G^GTACC
AcsI 2374 AAATTC [GA]^AATT[CT]
AflII 1210 CTTAAG C^TTAAG
AhdI 2998 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2374 AAATTC [GA]^AATT[CT]
Asp718I 2459 GGTACC G^GTACC
AspEI 2998 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2780 CACGTG CAC^GTG
BfrI 1210 CTTAAG C^TTAAG
BsiEI 46 CGACCG CG[GA][CT]^CG
BstXI 2338 CCACCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2269 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2998 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3531 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 601 GTCAAC GT[CT]^[GA]AC
HindII 601 GTCAAC GT[CT]^[GA]AC
KpnI 2459 GGTACC GGTAC^C
NspI 3560 GCATGC [GA]CATG^[CT]
PmaCI 2780 CACGTG CAC^GTG
PmlI 2780 CACGTG CAC^GTG
SexAI 905 ACCTGGT A^CC[AT]GGT
SfiI 1034 GGCCACCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1136 CCCGGG CCC^GGG
SphI 3560 GCATGC GCATG^C
StuI 1497 AGGCCT AGG^CCT
XcmI 2420 CCAGTAATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1136 CCCGGG C^CCGGG
XmaI 1136 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 2779 ACACGT A^C[GA][CT]GT
3269 ACACGT
AspI 904 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1693 GACTCTGTC
BalI 2180 TGGCCA TGG^CCA
3506 TGGCCA
BbsI 1690 GAAGAC GAAGAC(2/6)
2143 GAAGAC
BciVI 222 GGATAC GTATCC(6/5)
3433 GGATAC
BpuAI 1690 GAAGAC GAAGAC(2/6)
2143 GAAGAC
BsgI 2606 CTGCAC GTGCAG(16/14)
3265 CTGCAC
BsrDI 2795 CATTGC GCAATG(2/0)
1868 GCAATG
BsrFI 687 GCCGGC [GA]^CCGG[CT]
2230 GCCGGC
BstAPI 177 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3555 GCAGTGCATGC
Cfr10I 687 GCCGGC [GA]^CCGG[CT]
2230 GCCGGC
MaeIII 2776 GTGAC ^GT[ACGT]AC
3605 GTGAC
MluNI 2180 TGGCCA TGG^CCA
3506 TGGCCA
MscI 2180 TGGCCA TGG^CCA
3506 TGGCCA
MseI 1211 TTAA T^TAA
1337 TTAA
NaeI 687 GCCGGC GCC^GGC
2230 GCCGGC
NcoI 105 CCATGG C^CATGG
383 CCATGG
NgoMIV 687 GCCGGC G^CCGGC
2230 GCCGGC
PflFI 904 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1693 GACTCTGTC
SbfI 1716 CCTGCAGG CCTGCA^GG
3252 CCTGCAGG
Sse8387I 1716 CCTGCAGG CCTGCA^GG
3252 CCTGCAGG
Tru9I 1211 TTAA T^TAA
1337 TTAA
Tsp45I 2776 GTGAC ^GT[GC]AC
3605 GTGAC
Tth111I 904 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1693 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BamHI BanI
BanII BbeI BbvCI BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeII MboI MboII MflI
MnlI MroI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PaeR7I PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TseI
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII