Restriction map for
pF1KA1193
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BbeI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsiWI BsmI BspDI
BspHI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MscI
MseI MslI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Aor51HI 1043 AGCGCT AGC^GCT
BamHI 1473 GGATCC G^GATCC
BanI 194 GGTGCC G^G[CT][GA]CC
BbrPI 1575 CACGTG CAC^GTG
BglII 653 AGATCT A^GATCT
BsaAI 1575 CACGTG [CT]AC^GT[GA]
BsmBI 412 GAGACG CGTCTC(1/5)
Bsp1407I 725 TGTACA T^GTACA
BsrGI 725 TGTACA T^GTACA
BsrI 1183 ACTGG ACTGG(1/-1)
BstEII 1515 GGTCACC G^GT[ACGT]ACC
BstPI 1515 GGTCACC G^GT[ACGT]ACC
BstXI 1451 CCAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 685 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1043 AGCGCT AGC^GCT
EcoO65I 1515 GGTCACC G^GT[ACGT]ACC
NcoI 107 CCATGG C^CATGG
NspI 251 ACATGC [GA]CATG^[CT]
PmaCI 1575 CACGTG CAC^GTG
PmlI 1575 CACGTG CAC^GTG
PshAI 1259 GACTCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 527 GCTCTTC GCTCTTC(1/4)
ScaI 1016 AGTACT AGT^ACT
SfaNI 1188 GATGC GCATC(5/9)
SfcI 159 CTACAG C^T[GA][CT]AG
SmaI 1588 CCCGGG CCC^GGG
SspBI 725 TGTACA T^GTACA
XmaCI 1588 CCCGGG C^CCGGG
XmaI 1588 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 291 GGCGTC G[GA]^CG[CT]C
1393 GACGCC
Asp700I 225 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1551 GAACCCCTTC
AspHI 55 GAGCAC G[AT]GC[AT]^C
931 GAGCAC
AviII 986 TGCGCA TGC^GCA
1190 TGCGCA
BbsI 511 GAAGAC GAAGAC(2/6)
667 GAAGAC
BcgI 380 CGAAGGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
27 GCAGAGTCCTCG
BpuAI 511 GAAGAC GAAGAC(2/6)
667 GAAGAC
BsaHI 291 GGCGTC G[GA]^CG[CT]C
1393 GACGCC
BsgI 628 CTGCAC GTGCAG(16/14)
918 GTGCAG
BsiEI 1152 CGGCCG CG[GA][CT]^CG
1220 CGGCCG
BsiHKAI 55 GAGCAC G[AT]GC[AT]^C
931 GAGCAC
BspMI 626 ACCTGC ACCTGC(4/8)
1630 ACCTGC
BsrFI 638 ACCGGC [GA]^CCGG[CT]
1222 GCCGGT
BssSI 457 CACGAG CACGAG(-5/-1)
751 CACGAG
Cfr10I 638 ACCGGC [GA]^CCGG[CT]
1222 GCCGGT
EagI 1152 CGGCCG C^GGCCG
1220 CGGCCG
EclXI 1152 CGGCCG C^GGCCG
1220 CGGCCG
Eco52I 1152 CGGCCG C^GGCCG
1220 CGGCCG
EcoNI 966 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1425 CCTGCCCAAGG
FauI 1312 CCCGC CCCGC(4/6)
1469 GCGGG
FspI 986 TGCGCA TGC^GCA
1190 TGCGCA
HaeII 807 GGCGCT [GA]GCGC^[CT]
1043 AGCGCT
Hin1I 291 GGCGTC G[GA]^CG[CT]C
1393 GACGCC
PaeR7I 52 CTCGAG C^TCGAG
189 CTCGAG
PflMI 1119 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1293 CCAGCAGCTGG
SmlI 52 CTCGAG C^T[CT][GA]AG
189 CTCGAG
Van91I 1119 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1293 CCAGCAGCTGG
XhoI 52 CTCGAG C^TCGAG
189 CTCGAG
XmnI 225 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1551 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AvaI AvaII BanII
BbvCI BbvI BcnI BmyI BpmI
BsaI BsaJI BsaWI BseAI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MroI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII