Restriction map for
pF1KSDA1187
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BamHI
BbvCI BcgI BciVI BclI BfrI
BglII BsaBI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstYI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I XbaI XhoII
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# ONE CUT ENZYMES ( 33 enzymes) #
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AfaI 453 GTAC GT^AC
Alw44I 1024 GTGCAC G^TGCAC
AlwI 780 GGATC GGATC(4/5)
AocI 212 CCTCAGG CC^T[ACGT]AGG
ApaLI 1024 GTGCAC G^TGCAC
Asp700I 2274 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 229 CCTAGG C^CTAGG
BbsI 660 GAAGAC GAAGAC(2/6)
BlnI 229 CCTAGG C^CTAGG
BpuAI 660 GAAGAC GAAGAC(2/6)
BsaI 1585 GAGACC GGTCTC(1/5)
BsaWI 1092 TCCGGT [AT]^CCGG[AT]
BspHI 2033 TCATGA T^CATGA
BssHII 919 GCGCGC G^CGCGC
BssSI 1862 CACGAG CACGAG(-5/-1)
Bsu36I 212 CCTCAGG CC^T[ACGT]AGG
Eco81I 212 CCTCAGG CC^T[ACGT]AGG
KspI 1580 CCGCGG CCGC^GG
MseI 560 TTAA T^TAA
NcoI 299 CCATGG C^CATGG
PaeR7I 610 CTCGAG C^TCGAG
PflMI 1301 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2305 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2033 TCATGA T^CATGA
RsaI 453 GTAC GT^AC
SacI 1261 GAGCTC GAGCT^C
SacII 1580 CCGCGG CCGC^GG
SapI 439 GAAGAGC GCTCTTC(1/4)
Tru9I 560 TTAA T^TAA
Van91I 1301 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1685 CCACTCGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 610 CTCGAG C^TCGAG
XmnI 2274 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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Aor51HI 626 AGCGCT AGC^GCT
686 AGCGCT
AspHI 1024 GTGCAC G[AT]GC[AT]^C
1261 GAGCTC
BbrPI 891 CACGTG CAC^GTG
1229 CACGTG
BlpI 753 GCTGAGC GC^T[ACGT]AGC
1263 GCTCAGC
Bpu1102I 753 GCTGAGC GC^T[ACGT]AGC
1263 GCTCAGC
BsaAI 891 CACGTG [CT]AC^GT[GA]
1229 CACGTG
BsiHKAI 1024 GTGCAC G[AT]GC[AT]^C
1261 GAGCTC
BsmI 168 GAATGC GAATGC(1/-1)
1778 GAATGC
BspMI 219 GCAGGT ACCTGC(4/8)
2391 GCAGGT
BstEII 91 GGTGACC G^GT[ACGT]ACC
1638 GGTGACC
BstPI 91 GGTGACC G^GT[ACGT]ACC
1638 GGTGACC
BstXI 449 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2268 CCAGGAGAATGG
CelII 753 GCTGAGC GC^T[ACGT]AGC
1263 GCTCAGC
DpnII 781 GATC ^GATC
2031 GATC
DrdI 1597 GACGCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2043 GACTCGGAAGTC
EagI 849 CGGCCG C^GGCCG
1105 CGGCCG
EclXI 849 CGGCCG C^GGCCG
1105 CGGCCG
Eco47III 626 AGCGCT AGC^GCT
686 AGCGCT
Eco52I 849 CGGCCG C^GGCCG
1105 CGGCCG
EcoNI 877 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1307 CCTGGCACAGG
EcoO65I 91 GGTGACC G^GT[ACGT]ACC
1638 GGTGACC
MboI 781 GATC ^GATC
2031 GATC
NdeII 781 GATC ^GATC
2031 GATC
PmaCI 891 CACGTG CAC^GTG
1229 CACGTG
PmlI 891 CACGTG CAC^GTG
1229 CACGTG
PpuMI 58 AGGACCC [GA]G^G[AT]CC[CT]
282 AGGACCC
Sau3AI 781 GATC ^GATC
2031 GATC
StuI 1112 AGGCCT AGG^CCT
1315 AGGCCT
TaqI 327 TCGA T^CGA
611 TCGA
TthHB8I 327 TCGA T^CGA
611 TCGA
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIII NlaIV PleI
PstI PvuII Sau96I ScrFI SfaNI
SfcI SfoI SgrAI SmaI SmlI
StyI TseI Tsp45I TspRI XmaCI
XmaI