Restriction map for
pF1KSDA1186
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MslI MunI
MvnI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 167 TCCGGA T^CCGGA
AocI 906 CCTCAGG CC^T[ACGT]AGG
BalI 47 TGGCCA TGG^CCA
BanII 24 GAGCCC G[GA]GC[CT]^C
BbsI 960 GAAGAC GAAGAC(2/6)
BbvCI 360 GCTGAGG CCTCAGC(-5/-2)
BpmI 227 CTCCAG CTGGAG(16/14)
BpuAI 960 GAAGAC GAAGAC(2/6)
BsaWI 167 TCCGGA [AT]^CCGG[AT]
BseAI 167 TCCGGA T^CCGGA
BseRI 861 CTCCTC GAGGAG(10/8)
BsgI 844 GTGCAG GTGCAG(16/14)
BspEI 167 TCCGGA T^CCGGA
BsrFI 81 GCCGGC [GA]^CCGG[CT]
BsrI 289 CCAGT ACTGG(1/-1)
BstXI 416 CCACAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 884 AGATCC [GA]^GATC[CT]
Bsu36I 906 CCTCAGG CC^T[ACGT]AGG
Cfr10I 81 GCCGGC [GA]^CCGG[CT]
DsaI 855 CCGTGG C^C[GA][CT]GG
EaeI 47 TGGCCA [CT]^GGCC[GA]
Eco81I 906 CCTCAGG CC^T[ACGT]AGG
EcoNI 624 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 323 CCAAGG C^C[AT][AT]GG
HgaI 561 GCGTC GACGC(5/10)
HindIII 135 AAGCTT A^AGCTT
MaeIII 785 GTGAC ^GT[ACGT]AC
MflI 884 AGATCC [GA]^GATC[CT]
MluNI 47 TGGCCA TGG^CCA
MroI 167 TCCGGA T^CCGGA
MscI 47 TGGCCA TGG^CCA
MseI 575 TTAA T^TAA
NaeI 81 GCCGGC GCC^GGC
NgoMIV 81 GCCGGC G^CCGGC
PflMI 867 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 11 GAGTC GAGTC(4/5)
PvuII 1186 CAGCTG CAG^CTG
SmlI 478 CTCAAG C^T[CT][GA]AG
StuI 506 AGGCCT AGG^CCT
StyI 323 CCAAGG C^C[AT][AT]GG
TaqI 140 TCGA T^CGA
TfiI 93 GAATC G^A[AT]TC
Tru9I 575 TTAA T^TAA
Tsp45I 785 GTGAC ^GT[GC]AC
TthHB8I 140 TCGA T^CGA
Van91I 867 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 884 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 297 GTAC GT^AC
1023 GTAC
AlwNI 244 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
352 CAGGACCTG
BsaI 439 GAGACC GGTCTC(1/5)
812 GAGACC
BsmAI 439 GAGAC GTCTC(1/5)
812 GAGAC
BsmFI 1051 GGGAC GGGAC(10/14)
1039 GTCCC
BsrBI 3 GAGCGG CCGCTC(-3/-3)
932 GAGCGG
DraIII 838 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
868 CACCAAGTG
EarI 889 CTCTTC CTCTTC(1/4)
1171 GAAGAG
FauI 375 GCGGG CCCGC(4/6)
984 GCGGG
HapII 82 CCGG C^CGG
168 CCGG
HinfI 11 GAGTC G^A[ACGT]TC
93 GAATC
HpaII 82 CCGG C^CGG
168 CCGG
Ksp632I 889 CTCTTC CTCTTC(1/4)
1171 GAAGAG
MspA1I 982 CAGCGG C[AC]G^C[GT]G
1186 CAGCTG
MspI 82 CCGG C^CGG
168 CCGG
NspI 174 GCATGT [GA]CATG^[CT]
527 GCATGT
PstI 145 CTGCAG CTGCA^G
1062 CTGCAG
RsaI 297 GTAC GT^AC
1023 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HphI ItaI MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PpuMI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI TseI Tsp509I TspRI