Restriction map for
pF1KSDA1165
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# NO CUT ENZYMES ( 183 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FokI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tsp45I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 338 GAATTC [GA]^AATT[CT]
AflIII 674 ACATGT A^C[GA][CT]GT
ApoI 338 GAATTC [GA]^AATT[CT]
BamHI 542 GGATCC G^GATCC
BbsI 126 GTCTTC GAAGAC(2/6)
BclI 533 TGATCA T^GATCA
BcnI 16 CCGGG CC^[GC]GG
BpuAI 126 GTCTTC GAAGAC(2/6)
BsaJI 16 CCGGGG C^C[ACGT][ACGT]GG
BsgI 115 GTGCAG GTGCAG(16/14)
BsmFI 19 GGGAC GGGAC(10/14)
BspLU11I 674 ACATGT A^CATGT
BsrDI 304 GCAATG GCAATG(2/0)
BstAPI 108 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 33 CCAGG CC^[AT]GG
BstYI 542 GGATCC [GA]^GATC[CT]
CfoI 27 GCGC GCG^C
EcoRI 338 GAATTC G^AATTC
EcoRII 33 CCAGG ^CC[AT]GG
FbaI 533 TGATCA T^GATCA
HaeII 26 GGCGCT [GA]GCGC^[CT]
HapII 16 CCGG C^CGG
HhaI 27 GCGC GCG^C
HinP1I 27 GCGC G^CGC
HpaII 16 CCGG C^CGG
MflI 542 GGATCC [GA]^GATC[CT]
MslI 411 CATTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 16 CCGG C^CGG
MvaI 33 CCAGG CC^[AT]GG
NciI 16 CCGGG CC^[GC]GG
NlaIV 542 GGATCC GG[ACGT]^[ACGT]CC
NspI 674 ACATGT [GA]CATG^[CT]
PleI 151 GACTC GAGTC(4/5)
PstI 314 CTGCAG CTGCA^G
SexAI 32 ACCAGGT A^CC[AT]GGT
SfaNI 379 GATGC GCATC(5/9)
TfiI 64 GAATC G^A[AT]TC
TspRI 375 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 12 CCAGCCGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 542 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 106 CCGC CCGC(-3/-1)
515 GCGG
AfaI 190 GTAC GT^AC
619 GTAC
BsiYI 9 CCACCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
16 CCGGGGACTGG
BslI 9 CCACCAGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
16 CCGGGGACTGG
BssKI 16 CCGGG ^CC[ACGT]GG
33 CCAGG
HaeIII 523 GGCC GG^CC
625 GGCC
HinfI 64 GAATC G^A[ACGT]TC
151 GACTC
MseI 44 TTAA T^TAA
653 TTAA
MspA1I 311 CAGCTG C[AC]G^C[GT]G
692 CAGCTG
PvuII 311 CAGCTG CAG^CTG
692 CAGCTG
RsaI 190 GTAC GT^AC
619 GTAC
ScrFI 16 CCGGG CC^[ACGT]GG
33 CCAGG
Tru9I 44 TTAA T^TAA
653 TTAA
Tsp509I 339 AATT ^AATT
655 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwI BbvI BsrI Cac8I
DdeI DpnII EarI Fnu4HI HphI
ItaI Ksp632I MboI MboII MnlI
MwoI NdeII NlaIII Sau3AI SfcI
TseI