Restriction map for
pF1KSDA1138
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# NO CUT ENZYMES ( 77 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AgeI AhdI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BciVI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SfuI SnaBI SpeI
SspI SwaI Tsp509I Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AatII 3370 GACGTC GACGT^C
AccI 3244 GTCGAC GT^[AC][GT]AC
Alw44I 3256 GTGCAC G^TGCAC
ApaLI 3256 GTGCAC G^TGCAC
AscI 118 GGCGCGCC GG^CGCGCC
AviII 293 TGCGCA TGC^GCA
Bsp1407I 2774 TGTACA T^GTACA
BspLU11I 3041 ACATGT A^CATGT
BsrGI 2774 TGTACA T^GTACA
BssHII 119 GCGCGC G^CGCGC
DraI 2397 TTTAAA TTT^AAA
DrdI 3349 GACACAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 293 TGCGCA TGC^GCA
NcoI 1835 CCATGG C^CATGG
NdeI 3212 CATATG CA^TATG
SacI 3151 GAGCTC GAGCT^C
SalI 3244 GTCGAC G^TCGAC
SbfI 2906 CCTGCAGG CCTGCA^GG
ScaI 536 AGTACT AGT^ACT
SgrAI 3603 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3614 GCATGC GCATG^C
Sse8387I 2906 CCTGCAGG CCTGCA^GG
SspBI 2774 TGTACA T^GTACA
TfiI 2369 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AlwNI 2302 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2910 CAGGACCTG
AocI 1334 CCTCAGG CC^T[ACGT]AGG
2784 CCTCAGG
BalI 1328 TGGCCA TGG^CCA
2298 TGGCCA
BbrPI 3094 CACGTG CAC^GTG
3259 CACGTG
BbsI 3135 GAAGAC GAAGAC(2/6)
3643 GAAGAC
BcgI 2117 CGAGCTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2146 GCAGAGAAGTCG
BfaI 1474 CTAG C^TAG
1498 CTAG
BlpI 2724 GCTCAGC GC^T[ACGT]AGC
3387 GCTGAGC
Bpu1102I 2724 GCTCAGC GC^T[ACGT]AGC
3387 GCTGAGC
BpuAI 3135 GAAGAC GAAGAC(2/6)
3643 GAAGAC
BsaI 1006 GAGACC GGTCTC(1/5)
3031 GAGACC
BsgI 1024 GTGCAG GTGCAG(16/14)
3097 GTGCAG
BsmBI 340 GAGACG CGTCTC(1/5)
1015 GAGACG
BssSI 2677 CACGAG CACGAG(-5/-1)
2941 CTCGTG
BstAPI 2909 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3090 GCAACACGTGC
Bsu36I 1334 CCTCAGG CC^T[ACGT]AGG
2784 CCTCAGG
CelII 2724 GCTCAGC GC^T[ACGT]AGC
3387 GCTGAGC
DraIII 2677 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3619 CACCTGGTG
Eco81I 1334 CCTCAGG CC^T[ACGT]AGG
2784 CCTCAGG
HincII 271 GTCAAC GT[CT]^[GA]AC
3244 GTCGAC
HindII 271 GTCAAC GT[CT]^[GA]AC
3244 GTCGAC
MaeI 1474 CTAG C^TAG
1498 CTAG
MluNI 1328 TGGCCA TGG^CCA
2298 TGGCCA
MscI 1328 TGGCCA TGG^CCA
2298 TGGCCA
MseI 475 TTAA T^TAA
2398 TTAA
PmaCI 3094 CACGTG CAC^GTG
3259 CACGTG
PmlI 3094 CACGTG CAC^GTG
3259 CACGTG
PshAI 556 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3228 GACGCGCGTC
SapI 1446 GAAGAGC GCTCTTC(1/4)
3489 GCTCTTC
SexAI 3313 ACCAGGT A^CC[AT]GGT
3620 ACCTGGT
SfiI 279 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1349 GGCCTGACCGGCC
StuI 1348 AGGCCT AGG^CCT
1712 AGGCCT
Tru9I 475 TTAA T^TAA
2398 TTAA
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# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI Aor51HI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI