Restriction map for
pF1KSDA1095
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspI
AvrII BalI BbeI BbrPI BcgI
BclI BfrI BlnI BsaAI BseAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI BstBI BstEII BstPI
Bsu36I ClaI DraI DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoT22I FbaI
HpaI KasI KpnI MfeI MluNI
MroI MscI MunI NarI NcoI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI SalI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 3166 GTATAC GT^[AC][GT]AC
AflIII 395 ACGCGT A^C[GA][CT]GT
AseI 1417 ATTAAT AT^TAAT
AsnI 1417 ATTAAT AT^TAAT
BanI 2452 GGCACC G^G[CT][GA]CC
BbvCI 1172 CCTCAGC CCTCAGC(-5/-2)
BciVI 847 GTATCC GTATCC(6/5)
BglII 2315 AGATCT A^GATCT
BlpI 687 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 687 GCTCAGC GC^T[ACGT]AGC
BsaI 1400 GAGACC GGTCTC(1/5)
BsaWI 1310 ACCGGA [AT]^CCGG[AT]
BsiWI 248 CGTACG C^GTACG
Bst1107I 3166 GTATAC GTA^TAC
BstZ17I 3166 GTATAC GTA^TAC
CelII 687 GCTCAGC GC^T[ACGT]AGC
CpoI 1717 CGGACCG CG^G[AT]CCG
EcoNI 2943 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2224 GATATC GAT^ATC
FseI 796 GGCCGGCC GGCCGG^CC
HindIII 965 AAGCTT A^AGCTT
MluI 395 ACGCGT A^CGCGT
NheI 1618 GCTAGC G^CTAGC
PshAI 744 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1417 ATTAAT AT^TAAT
RsrII 1717 CGGACCG CG^G[AT]CCG
SexAI 2942 ACCTGGT A^CC[AT]GGT
SfiI 597 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 784 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 655 AAATTC [GA]^AATT[CT]
896 AAATTC
ApoI 655 AAATTC [GA]^AATT[CT]
896 AAATTC
Asp700I 651 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3093 GAAAATCTTC
AviII 2075 TGCGCA TGC^GCA
2204 TGCGCA
BamHI 1143 GGATCC G^GATCC
1910 GGATCC
BglI 425 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
598 GCCCAGCTGGC
BsrDI 1368 CATTGC GCAATG(2/0)
1490 CATTGC
BssSI 1693 CACGAG CACGAG(-5/-1)
2212 CACGAG
BstAPI 285 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2354 GCATCAGCTGC
BstXI 1413 CCAGATTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1790 CCAACCCGCTGG
FspI 2075 TGCGCA TGC^GCA
2204 TGCGCA
HincII 859 GTTGAC GT[CT]^[GA]AC
919 GTCAAC
HindII 859 GTTGAC GT[CT]^[GA]AC
919 GTCAAC
KspI 168 CCGCGG CCGC^GG
608 CCGCGG
MaeIII 2027 GTGAC ^GT[ACGT]AC
3187 GTGAC
MseI 1418 TTAA T^TAA
3130 TTAA
PaeR7I 304 CTCGAG C^TCGAG
2262 CTCGAG
SacII 168 CCGCGG CCGC^GG
608 CCGCGG
StuI 888 AGGCCT AGG^CCT
1504 AGGCCT
Tru9I 1418 TTAA T^TAA
3130 TTAA
Tsp45I 2027 GTGAC ^GT[GC]AC
3187 GTGAC
XcmI 2492 CCAACCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2552 CCAGCCAGAAGCTGG
XhoI 304 CTCGAG C^TCGAG
2262 CTCGAG
XmnI 651 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3093 GAAAATCTTC
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# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MamI MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp509I TspRI TthHB8I Van91I XhoII