Restriction map for
pF1KSDA1094
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Aor51HI 629 AGCGCT AGC^GCT
ApaI 1307 GGGCCC GGGCC^C
AviII 1443 TGCGCA TGC^GCA
BalI 920 TGGCCA TGG^CCA
BglI 922 GCCACCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1467 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 911 GGTCTC GGTCTC(1/5)
BsmI 954 GAATGC GAATGC(1/-1)
BsrBI 289 GAGCGG CCGCTC(-3/-3)
BssSI 126 CTCGTG CACGAG(-5/-1)
BstYI 1388 AGATCC [GA]^GATC[CT]
Eco47III 629 AGCGCT AGC^GCT
FspI 1443 TGCGCA TGC^GCA
MaeII 164 ACGT A^CGT
MaeIII 1154 GTCAC ^GT[ACGT]AC
MamI 1467 GATCTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1388 AGATCC [GA]^GATC[CT]
MluNI 920 TGGCCA TGG^CCA
MscI 920 TGGCCA TGG^CCA
MseI 1471 TTAA T^TAA
NspI 1241 GCATGT [GA]CATG^[CT]
PpuMI 659 GGGACCC [GA]G^G[AT]CC[CT]
SfiI 921 GGCCACCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 27 CCCGGG CCC^GGG
SpeI 276 ACTAGT A^CTAGT
TaqI 494 TCGA T^CGA
TfiI 843 GAATC G^A[AT]TC
Tru9I 1471 TTAA T^TAA
Tsp45I 1154 GTCAC ^GT[GC]AC
TthHB8I 494 TCGA T^CGA
XcmI 1088 CCACTGTATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1388 AGATCC [GA]^GATC[CT]
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 344 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1269 CAGACCCTG
Asp700I 309 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
505 GAAGTCCTTC
AvaI 5 CCCGAG C^[CT]CG[GA]G
27 CCCGGG
BanI 293 GGTGCC G^G[CT][GA]CC
751 GGCACC
BanII 38 GGGCTC G[GA]GC[CT]^C
1307 GGGCCC
BcnI 27 CCCGG CC^[GC]GG
28 CCGGG
BfaI 277 CTAG C^TAG
901 CTAG
BlpI 93 GCTGAGC GC^T[ACGT]AGC
795 GCTGAGC
BpmI 440 CTGGAG CTGGAG(16/14)
1117 CTGGAG
Bpu1102I 93 GCTGAGC GC^T[ACGT]AGC
795 GCTGAGC
BseRI 762 CTCCTC GAGGAG(10/8)
1298 GAGGAG
BsoBI 5 CCCGAG C^[CT]CG[GA]G
27 CCCGGG
BspMI 548 ACCTGC ACCTGC(4/8)
1229 ACCTGC
BstXI 297 CCAGACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
347 CCACTGGCATGG
CelII 93 GCTGAGC GC^T[ACGT]AGC
795 GCTGAGC
EaeI 920 TGGCCA [CT]^GGCC[GA]
1066 CGGCCA
MaeI 277 CTAG C^TAG
901 CTAG
NciI 27 CCCGG CC^[GC]GG
28 CCGGG
NcoI 254 CCATGG C^CATGG
1379 CCATGG
PleI 745 GACTC GAGTC(4/5)
976 GAGTC
XmnI 309 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
505 GAAGTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbsI BbvI BmyI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI TspRI