Restriction map for
pF1KSDA1092
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# NO CUT ENZYMES ( 84 enzymes) #
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AatII AccI AccIII AflIII AgeI
AhdI Aor51HI AscI AspEI AviII
AvrII BamHI BbsI BbvCI BcgI
BclI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoNI
EcoO65I FbaI FseI FspI HincII
HindII HpaI MamI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PvuI PvuII RsrII
SalI SapI SbfI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
StuI SwaI Van91I XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AclI 2006 AACGTT AA^CGTT
AflII 928 CTTAAG C^TTAAG
Alw44I 2404 GTGCAC G^TGCAC
AocI 513 CCTAAGG CC^T[ACGT]AGG
ApaLI 2404 GTGCAC G^TGCAC
AseI 418 ATTAAT AT^TAAT
AsnI 418 ATTAAT AT^TAAT
AspI 2236 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2629 CACGTG CAC^GTG
BciVI 1536 GTATCC GTATCC(6/5)
BfrI 928 CTTAAG C^TTAAG
BsaAI 2629 CACGTG [CT]AC^GT[GA]
BsgI 2381 GTGCAG GTGCAG(16/14)
BsmBI 2417 GAGACG CGTCTC(1/5)
BspMI 2459 ACCTGC ACCTGC(4/8)
BsrFI 28 GCCGGC [GA]^CCGG[CT]
BssHII 139 GCGCGC G^CGCGC
Bsu36I 513 CCTAAGG CC^T[ACGT]AGG
Cfr10I 28 GCCGGC [GA]^CCGG[CT]
DraI 906 TTTAAA TTT^AAA
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
Eco81I 513 CCTAAGG CC^T[ACGT]AGG
EcoRV 633 GATATC GAT^ATC
HgaI 2419 GACGC GACGC(5/10)
HindIII 2439 AAGCTT A^AGCTT
KspI 228 CCGCGG CCGC^GG
NaeI 28 GCCGGC GCC^GGC
NgoMIV 28 GCCGGC G^CCGGC
NheI 208 GCTAGC G^CTAGC
PflFI 2236 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2629 CACGTG CAC^GTG
PmlI 2629 CACGTG CAC^GTG
PshBI 418 ATTAAT AT^TAAT
Psp1406I 2006 AACGTT AA^CGTT
SacI 1382 GAGCTC GAGCT^C
SacII 228 CCGCGG CCGC^GG
ScaI 1922 AGTACT AGT^ACT
SgrAI 27 CGCCGGCG C[GA]^CCGG[CT]G
SphI 377 GCATGC GCATG^C
Tth111I 2236 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 466 TCTAGA T^CTAGA
XcmI 2321 CCAAAGGTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 740 GGTACC G^GTACC
967 GGTACC
AlwNI 1866 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
3053 CAGATGCTG
Asp700I 1147 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2042 GAATTGATTC
Asp718I 740 GGTACC G^GTACC
967 GGTACC
AspHI 1382 GAGCTC G[AT]GC[AT]^C
2404 GTGCAC
BalI 2471 TGGCCA TGG^CCA
2858 TGGCCA
BglI 14 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
28 GCCGGCGGGGC
BglII 2793 AGATCT A^GATCT
3213 AGATCT
BpmI 2594 CTGGAG CTGGAG(16/14)
3374 CTGGAG
BsaI 2208 GGTCTC GGTCTC(1/5)
2250 GGTCTC
BsiEI 12 CGGCCG CG[GA][CT]^CG
260 CGGTCG
BsiHKAI 1382 GAGCTC G[AT]GC[AT]^C
2404 GTGCAC
BspHI 1005 TCATGA T^CATGA
2195 TCATGA
BsrDI 614 GCAATG GCAATG(2/0)
879 GCAATG
BstAPI 706 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1519 GCATTCTTTGC
EarI 342 GAAGAG CTCTTC(1/4)
2648 GAAGAG
EcoT22I 1517 ATGCAT ATGCA^T
2714 ATGCAT
KpnI 740 GGTACC GGTAC^C
967 GGTACC
Ksp632I 342 GAAGAG CTCTTC(1/4)
2648 GAAGAG
MaeII 2007 ACGT A^CGT
2630 ACGT
MluNI 2471 TGGCCA TGG^CCA
2858 TGGCCA
MscI 2471 TGGCCA TGG^CCA
2858 TGGCCA
MspA1I 150 CAGCGG C[AC]G^C[GT]G
228 CCGCGG
NsiI 1517 ATGCAT ATGCA^T
2714 ATGCAT
PleI 2236 GACTC GAGTC(4/5)
679 GAGTC
RcaI 1005 TCATGA T^CATGA
2195 TCATGA
SspI 2140 AATATT AAT^ATT
3187 AATATT
TaqI 1250 TCGA T^CGA
1380 TCGA
TthHB8I 1250 TCGA T^CGA
1380 TCGA
XmnI 1147 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2042 GAATTGATTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BmyI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmaCI XmaI