Restriction map for
pF1KSDA1089
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# NO CUT ENZYMES ( 95 enzymes) #
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AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
ApoI AscI AseI AsnI AspEI
AvrII BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspEI BspLU11I
BsrFI BsrGI BssHII BssSI BstBI
BstUI Cfr10I CpoI EagI Eam1105I
EclXI Eco52I EcoNI EcoRI EcoT22I
FbaI FseI Hin1I HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 2720 GGTACC G^GTACC
Alw44I 1096 GTGCAC G^TGCAC
Aor51HI 1733 AGCGCT AGC^GCT
ApaLI 1096 GTGCAC G^TGCAC
Asp700I 1974 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2720 GGTACC G^GTACC
AspI 1599 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1978 GTCTTC GAAGAC(2/6)
BglII 2133 AGATCT A^GATCT
BpuAI 1978 GTCTTC GAAGAC(2/6)
BspDI 2665 ATCGAT AT^CGAT
BspHI 2471 TCATGA T^CATGA
Bst1107I 256 GTATAC GTA^TAC
BstZ17I 256 GTATAC GTA^TAC
ClaI 2665 ATCGAT AT^CGAT
DraI 2737 TTTAAA TTT^AAA
DraIII 208 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1898 GACGCTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 911 CCGTGG C^C[GA][CT]GG
Eco47III 1733 AGCGCT AGC^GCT
EcoRV 548 GATATC GAT^ATC
HaeII 1733 AGCGCT [GA]GCGC^[CT]
HincII 1183 GTCAAC GT[CT]^[GA]AC
HindII 1183 GTCAAC GT[CT]^[GA]AC
KpnI 2720 GGTACC GGTAC^C
NspI 508 GCATGT [GA]CATG^[CT]
PflFI 1599 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2362 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1940 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 2471 TCATGA T^CATGA
SapI 2197 GCTCTTC GCTCTTC(1/4)
SbfI 2973 CCTGCAGG CCTGCA^GG
ScaI 674 AGTACT AGT^ACT
SexAI 341 ACCTGGT A^CC[AT]GGT
SfiI 1660 GGCCCACTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2973 CCTGCAGG CCTGCA^GG
Tth111I 1599 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2362 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1974 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 256 GTATAC GT^[AC][GT]AC
1834 GTAGAC
ApaI 1668 GGGCCC GGGCC^C
2702 GGGCCC
AviII 2025 TGCGCA TGC^GCA
2291 TGCGCA
BalI 1004 TGGCCA TGG^CCA
2621 TGGCCA
BamHI 1117 GGATCC G^GATCC
2892 GGATCC
BanI 655 GGCACC G^G[CT][GA]CC
2720 GGTACC
BbvCI 996 CCTCAGC CCTCAGC(-5/-2)
232 GCTGAGG
BsgI 205 CTGCAC GTGCAG(16/14)
1133 GTGCAG
BsmI 635 GAATGC GAATGC(1/-1)
1234 GCATTC
BsrBI 2215 CCGCTC CCGCTC(-3/-3)
2791 GAGCGG
BstAPI 1135 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2294 GCATCTGCTGC
BstEII 1604 GGTCACC G^GT[ACGT]ACC
2907 GGTGACC
BstPI 1604 GGTCACC G^GT[ACGT]ACC
2907 GGTGACC
BstXI 1239 CCAGAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2097 CCAGGAGTCTGG
EcoO65I 1604 GGTCACC G^GT[ACGT]ACC
2907 GGTGACC
FauI 2214 CCCGC CCCGC(4/6)
2330 CCCGC
FspI 2025 TGCGCA TGC^GCA
2291 TGCGCA
HindIII 193 AAGCTT A^AGCTT
1933 AAGCTT
MluNI 1004 TGGCCA TGG^CCA
2621 TGGCCA
MscI 1004 TGGCCA TGG^CCA
2621 TGGCCA
NheI 585 GCTAGC G^CTAGC
1681 GCTAGC
PshAI 429 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1492 GACAAGAGTC
SacI 181 GAGCTC GAGCT^C
2031 GAGCTC
SmaI 1460 CCCGGG CCC^GGG
2705 CCCGGG
SmlI 754 CTCAAG C^T[CT][GA]AG
1443 CTCAAG
StuI 1813 AGGCCT AGG^CCT
2922 AGGCCT
Tsp509I 560 AATT ^AATT
3042 AATT
XcmI 992 CCAACCTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1268 CCAAGGAACCTGTGG
XmaCI 1460 CCCGGG C^CCGGG
2705 CCCGGG
XmaI 1460 CCCGGG C^CCGGG
2705 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII