Restriction map for
pF1KSDA1075
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
BfrI BsaBI BsiWI Bsp1407I BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
CpoI DraI Eco47III EcoRI EcoRV
EcoT22I HpaI KspI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I RcaI RsrII
SacII SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI XbaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 116 TCCGGA T^CCGGA
AcsI 796 AAATTC [GA]^AATT[CT]
AflIII 1099 ACATGT A^C[GA][CT]GT
AhdI 2782 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2959 CCTCAGG CC^T[ACGT]AGG
ApoI 796 AAATTC [GA]^AATT[CT]
AspEI 2782 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3728 TGCGCA TGC^GCA
BbrPI 673 CACGTG CAC^GTG
BbsI 1261 GTCTTC GAAGAC(2/6)
BcgI 3051 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3488 TGATCA T^GATCA
BpuAI 1261 GTCTTC GAAGAC(2/6)
BseAI 116 TCCGGA T^CCGGA
BsmBI 1678 GAGACG CGTCTC(1/5)
BsmI 3731 GCATTC GAATGC(1/-1)
BspDI 157 ATCGAT AT^CGAT
BspEI 116 TCCGGA T^CCGGA
BspLU11I 1099 ACATGT A^CATGT
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
Bsu36I 2959 CCTCAGG CC^T[ACGT]AGG
ClaI 157 ATCGAT AT^CGAT
DrdI 1309 GACCCCTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1588 CGGCCG C^GGCCG
Eam1105I 2782 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1588 CGGCCG C^GGCCG
Eco52I 1588 CGGCCG C^GGCCG
Eco81I 2959 CCTCAGG CC^T[ACGT]AGG
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
FbaI 3488 TGATCA T^GATCA
FseI 1585 GGCCGGCC GGCCGG^CC
FspI 3728 TGCGCA TGC^GCA
HincII 1324 GTCGAC GT[CT]^[GA]AC
HindII 1324 GTCGAC GT[CT]^[GA]AC
HindIII 709 AAGCTT A^AGCTT
MroI 116 TCCGGA T^CCGGA
NcoI 641 CCATGG C^CATGG
NdeI 2402 CATATG CA^TATG
PaeR7I 3059 CTCGAG C^TCGAG
PmaCI 673 CACGTG CAC^GTG
PmlI 673 CACGTG CAC^GTG
PvuI 1933 CGATCG CGAT^CG
SacI 3150 GAGCTC GAGCT^C
SalI 1324 GTCGAC G^TCGAC
SexAI 2785 ACCAGGT A^CC[AT]GGT
SfiI 3055 GGCCCTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 600 GCATGC GCATG^C
Tsp509I 797 AATT ^AATT
XhoI 3059 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 2345 GGTACC G^GTACC
2567 GGTACC
AccI 1000 GTCTAC GT^[AC][GT]AC
1324 GTCGAC
Asp700I 108 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3990 GAAGCTCTTC
Asp718I 2345 GGTACC G^GTACC
2567 GGTACC
AspI 2816 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3855 GACAGAGTC
AvrII 76 CCTAGG C^CTAGG
1632 CCTAGG
BamHI 301 GGATCC G^GATCC
1882 GGATCC
BglII 749 AGATCT A^GATCT
4091 AGATCT
BlnI 76 CCTAGG C^CTAGG
1632 CCTAGG
BlpI 85 GCTGAGC GC^T[ACGT]AGC
2529 GCTGAGC
Bpu1102I 85 GCTGAGC GC^T[ACGT]AGC
2529 GCTGAGC
BsaAI 403 TACGTG [CT]AC^GT[GA]
673 CACGTG
BsiEI 1588 CGGCCG CG[GA][CT]^CG
1933 CGATCG
BsrBI 367 CCGCTC CCGCTC(-3/-3)
376 GAGCGG
CelII 85 GCTGAGC GC^T[ACGT]AGC
2529 GCTGAGC
KpnI 2345 GGTACC GGTAC^C
2567 GGTACC
MaeII 404 ACGT A^CGT
674 ACGT
MseI 562 TTAA T^TAA
974 TTAA
NaeI 1566 GCCGGC GCC^GGC
1586 GCCGGC
NgoMIV 1566 GCCGGC G^CCGGC
1586 GCCGGC
PflFI 2816 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3855 GACAGAGTC
Tru9I 562 TTAA T^TAA
974 TTAA
Tth111I 2816 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3855 GACAGAGTC
XmnI 108 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3990 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI