Restriction map for
pF1KSDA1056
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI AspI
BamHI BcgI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI HincII
HindII HpaI MamI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1531 GACGTC GACGT^C
Acc65I 31 GGTACC G^GTACC
AccII 1671 CGCG CG^CG
AcsI 557 AAATTC [GA]^AATT[CT]
AflIII 1319 ACATGT A^C[GA][CT]GT
Alw44I 1408 GTGCAC G^TGCAC
ApaLI 1408 GTGCAC G^TGCAC
ApoI 557 AAATTC [GA]^AATT[CT]
Asp700I 2148 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 31 GGTACC G^GTACC
AvaI 1947 CCCGGG C^[CT]CG[GA]G
AviII 1751 TGCGCA TGC^GCA
AvrII 1917 CCTAGG C^CTAGG
BbeI 1468 GGCGCC GGCGC^C
BbrPI 694 CACGTG CAC^GTG
BlnI 1917 CCTAGG C^CTAGG
BsoBI 1947 CCCGGG C^[CT]CG[GA]G
BspLU11I 1319 ACATGT A^CATGT
BssSI 1487 CTCGTG CACGAG(-5/-1)
BstUI 1671 CGCG CG^CG
BstXI 1896 CCAGTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 2373 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1751 TGCGCA TGC^GCA
HaeII 1468 GGCGCC [GA]GCGC^[CT]
KasI 1468 GGCGCC G^GCGCC
KpnI 31 GGTACC GGTAC^C
KspI 1670 CCGCGG CCGC^GG
MvnI 1671 CGCG CG^CG
NarI 1468 GGCGCC GG^CGCC
PmaCI 694 CACGTG CAC^GTG
PmlI 694 CACGTG CAC^GTG
PshAI 241 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1670 CCGCGG CCGC^GG
ScaI 1898 AGTACT AGT^ACT
SfoI 1468 GGCGCC GGC^GCC
SmaI 1947 CCCGGG CCC^GGG
SphI 2514 GCATGC GCATG^C
TfiI 1084 GAATC G^A[AT]TC
Tsp509I 558 AATT ^AATT
XmaCI 1947 CCCGGG C^CCGGG
XmaI 1947 CCCGGG C^CCGGG
XmnI 2148 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 1468 GGCGCC G[GA]^CG[CT]C
1531 GACGTC
AhdI 2134 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2479 GACTTTGAGTC
AocI 976 CCTGAGG CC^T[ACGT]AGG
1126 CCTGAGG
ApaI 1623 GGGCCC GGGCC^C
1906 GGGCCC
AspEI 2134 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2479 GACTTTGAGTC
AspHI 1408 GTGCAC G[AT]GC[AT]^C
2012 GAGCAC
BbsI 1228 GAAGAC GAAGAC(2/6)
703 GTCTTC
BpuAI 1228 GAAGAC GAAGAC(2/6)
703 GTCTTC
BsaAI 694 CACGTG [CT]AC^GT[GA]
2005 CACGTA
BsaHI 1468 GGCGCC G[GA]^CG[CT]C
1531 GACGTC
BsiHKAI 1408 GTGCAC G[AT]GC[AT]^C
2012 GAGCAC
BstYI 117 AGATCC [GA]^GATC[CT]
654 AGATCC
Bsu36I 976 CCTGAGG CC^T[ACGT]AGG
1126 CCTGAGG
Eam1105I 2134 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2479 GACTTTGAGTC
Eco81I 976 CCTGAGG CC^T[ACGT]AGG
1126 CCTGAGG
Hin1I 1468 GGCGCC G[GA]^CG[CT]C
1531 GACGTC
HindIII 867 AAGCTT A^AGCTT
2281 AAGCTT
MflI 117 AGATCC [GA]^GATC[CT]
654 AGATCC
MseI 11 TTAA T^TAA
2578 TTAA
NdeI 1576 CATATG CA^TATG
1759 CATATG
PpuMI 154 AGGTCCT [GA]G^G[AT]CC[CT]
1058 GGGACCC
SapI 451 GAAGAGC GCTCTTC(1/4)
545 GCTCTTC
Tru9I 11 TTAA T^TAA
2578 TTAA
XcmI 957 CCAGACAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1617 CCAGGCGGGCCCTGG
XhoII 117 AGATCC [GA]^GATC[CT]
654 AGATCC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TseI
Tsp45I TspRI