Restriction map for
pF1KSDA1052
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AvrII BclI BfrI BlnI
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 527 GACGTC GACGT^C
Acc65I 1409 GGTACC G^GTACC
AccII 149 CGCG CG^CG
Asp700I 3087 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1409 GGTACC G^GTACC
AspI 920 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2479 CACGTG CAC^GTG
BcgI 2214 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 462 GGATAC GTATCC(6/5)
BfaI 71 CTAG C^TAG
BglI 2827 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3186 AGATCT A^GATCT
BsaAI 2479 CACGTG [CT]AC^GT[GA]
BspLU11I 4136 ACATGT A^CATGT
BstUI 149 CGCG CG^CG
HincII 309 GTCAAC GT[CT]^[GA]AC
HindII 309 GTCAAC GT[CT]^[GA]AC
KpnI 1409 GGTACC GGTAC^C
MaeI 71 CTAG C^TAG
MseI 326 TTAA T^TAA
MvnI 149 CGCG CG^CG
NaeI 2753 GCCGGC GCC^GGC
NgoMIV 2753 GCCGGC G^CCGGC
NspI 4136 ACATGT [GA]CATG^[CT]
PaeR7I 1816 CTCGAG C^TCGAG
PflFI 920 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2479 CACGTG CAC^GTG
PmlI 2479 CACGTG CAC^GTG
PshAI 868 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1812 AGTACT AGT^ACT
SexAI 921 ACCTGGT A^CC[AT]GGT
Tru9I 326 TTAA T^TAA
Tth111I 920 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1816 CTCGAG C^TCGAG
XmnI 3087 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 1410 GTAC GT^AC
1813 GTAC
AflIII 4136 ACATGT A^C[GA][CT]GT
4248 ACGTGT
AviII 3441 TGCGCA TGC^GCA
3491 TGCGCA
BamHI 1680 GGATCC G^GATCC
3643 GGATCC
BbeI 2750 GGCGCC GGCGC^C
3422 GGCGCC
BsaWI 2662 ACCGGA [AT]^CCGG[AT]
4232 ACCGGA
BsmI 2229 GAATGC GAATGC(1/-1)
1635 GCATTC
BsrFI 826 GCCGGT [GA]^CCGG[CT]
2753 GCCGGC
BssSI 1438 CACGAG CACGAG(-5/-1)
2497 CACGAG
BstAPI 1863 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2877 GCAGCAGCTGC
BstEII 981 GGTGACC G^GT[ACGT]ACC
4268 GGTTACC
BstPI 981 GGTGACC G^GT[ACGT]ACC
4268 GGTTACC
Cfr10I 826 GCCGGT [GA]^CCGG[CT]
2753 GCCGGC
EcoNI 445 CCTTTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3501 CCTGGAGAAGG
EcoO65I 981 GGTGACC G^GT[ACGT]ACC
4268 GGTTACC
FspI 3441 TGCGCA TGC^GCA
3491 TGCGCA
HgaI 1141 GACGC GACGC(5/10)
2733 GCGTC
KasI 2750 GGCGCC G^GCGCC
3422 GGCGCC
MslI 256 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3520 CACCTGGATG
NarI 2750 GGCGCC GG^CGCC
3422 GGCGCC
NcoI 1224 CCATGG C^CATGG
1790 CCATGG
PpuMI 3200 AGGACCT [GA]G^G[AT]CC[CT]
3896 GGGACCC
RsaI 1410 GTAC GT^AC
1813 GTAC
SfoI 2750 GGCGCC GGC^GCC
3422 GGCGCC
SmaI 98 CCCGGG CCC^GGG
3893 CCCGGG
StuI 1395 AGGCCT AGG^CCT
3617 AGGCCT
XmaCI 98 CCCGGG C^CCGGG
3893 CCCGGG
XmaI 98 CCCGGG C^CCGGG
3893 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AcyI AhdI AluI
AlwI AlwNI AocI ApaI ApoI
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII