Restriction map for
pF1KSDA1036
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BbeI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
KspI MaeI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 1079 GGTACC G^GTACC
AccI 342 GTCTAC GT^[AC][GT]AC
AcsI 527 GAATTT [GA]^AATT[CT]
AcyI 169 GGCGTC G[GA]^CG[CT]C
AflIII 237 ACACGT A^C[GA][CT]GT
ApoI 527 GAATTT [GA]^AATT[CT]
Asp700I 597 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1079 GGTACC G^GTACC
AvaI 1029 CTCGGG C^[CT]CG[GA]G
BamHI 281 GGATCC G^GATCC
BbsI 1041 GAAGAC GAAGAC(2/6)
BbvCI 110 CCTCAGC CCTCAGC(-5/-2)
BlpI 705 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 705 GCTCAGC GC^T[ACGT]AGC
BpuAI 1041 GAAGAC GAAGAC(2/6)
BsaHI 169 GGCGTC G[GA]^CG[CT]C
BsaI 94 GAGACC GGTCTC(1/5)
BsaWI 950 ACCGGA [AT]^CCGG[AT]
BseRI 127 GAGGAG GAGGAG(10/8)
BsgI 373 GTGCAG GTGCAG(16/14)
BsiEI 741 CGGCCG CG[GA][CT]^CG
BsmBI 161 GAGACG CGTCTC(1/5)
BsoBI 1029 CTCGGG C^[CT]CG[GA]G
Bsp1407I 680 TGTACA T^GTACA
BsrGI 680 TGTACA T^GTACA
BsrI 984 CCAGT ACTGG(1/-1)
BssSI 715 CTCGTG CACGAG(-5/-1)
BstAPI 828 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 252 CCACCCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 705 GCTCAGC GC^T[ACGT]AGC
DrdI 163 GACGGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 285 CCGTGG C^C[GA][CT]GG
EagI 741 CGGCCG C^GGCCG
EclXI 741 CGGCCG C^GGCCG
Eco52I 741 CGGCCG C^GGCCG
HgaI 170 GCGTC GACGC(5/10)
Hin1I 169 GGCGTC G[GA]^CG[CT]C
HincII 184 GTCAAC GT[CT]^[GA]AC
HindII 184 GTCAAC GT[CT]^[GA]AC
HinfI 660 GAGTC G^A[ACGT]TC
KpnI 1079 GGTACC GGTAC^C
MslI 253 CACCCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 660 GAGTC GAGTC(4/5)
PstI 394 CTGCAG CTGCA^G
SacI 712 GAGCTC GAGCT^C
SapI 435 GAAGAGC GCTCTTC(1/4)
SfcI 394 CTGCAG C^T[GA][CT]AG
SphI 545 GCATGC GCATG^C
SspBI 680 TGTACA T^GTACA
TspRI 1014 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 597 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Aor51HI 377 AGCGCT AGC^GCT
560 AGCGCT
ApaI 927 GGGCCC GGGCC^C
980 GGGCCC
AspHI 712 GAGCTC G[AT]GC[AT]^C
757 GTGCTC
BalI 242 TGGCCA TGG^CCA
467 TGGCCA
BanI 40 GGTGCC G^G[CT][GA]CC
1079 GGTACC
BbrPI 238 CACGTG CAC^GTG
754 CACGTG
BcnI 189 CCGGG CC^[GC]GG
1089 CCGGG
BpmI 556 CTGGAG CTGGAG(16/14)
880 CTGGAG
BsaAI 238 CACGTG [CT]AC^GT[GA]
754 CACGTG
BsiHKAI 712 GAGCTC G[AT]GC[AT]^C
757 GTGCTC
BsmAI 94 GAGAC GTCTC(1/5)
161 GAGAC
BspMI 155 ACCTGC ACCTGC(4/8)
299 ACCTGC
Eco47III 377 AGCGCT AGC^GCT
560 AGCGCT
EcoT14I 470 CCAAGG C^C[AT][AT]GG
944 CCAAGG
MaeIII 789 GTCAC ^GT[ACGT]AC
1034 GTGAC
MluNI 242 TGGCCA TGG^CCA
467 TGGCCA
MscI 242 TGGCCA TGG^CCA
467 TGGCCA
MseI 431 TTAA T^TAA
1073 TTAA
NciI 189 CCGGG CC^[GC]GG
1089 CCGGG
NspI 545 GCATGC [GA]CATG^[CT]
899 ACATGC
PmaCI 238 CACGTG CAC^GTG
754 CACGTG
PmlI 238 CACGTG CAC^GTG
754 CACGTG
PpuMI 152 AGGACCT [GA]G^G[AT]CC[CT]
671 AGGACCT
SmlI 760 CTCAAG C^T[CT][GA]AG
907 CTCAAG
StyI 470 CCAAGG C^C[AT][AT]GG
944 CCAAGG
TaqI 728 TCGA T^CGA
818 TCGA
Tru9I 431 TTAA T^TAA
1073 TTAA
Tsp45I 789 GTCAC ^GT[GC]AC
1034 GTGAC
Tsp509I 429 AATT ^AATT
528 AATT
TthHB8I 728 TCGA T^CGA
818 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BanII BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI XhoII