Restriction map for
pF1KSDA1024
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BcgI BclI BfrI BglI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 562 CGCG CG^CG
AcsI 2647 GAATTC [GA]^AATT[CT]
AcyI 1229 GGCGCC G[GA]^CG[CT]C
Alw44I 1470 GTGCAC G^TGCAC
ApaI 1021 GGGCCC GGGCC^C
ApaLI 1470 GTGCAC G^TGCAC
ApoI 2647 GAATTC [GA]^AATT[CT]
Asp700I 771 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1090 TGGCCA TGG^CCA
BamHI 1636 GGATCC G^GATCC
BbeI 1229 GGCGCC GGCGC^C
BbrPI 1438 CACGTG CAC^GTG
BbvCI 616 CCTCAGC CCTCAGC(-5/-2)
BciVI 2614 GGATAC GTATCC(6/5)
BlpI 1398 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1398 GCTCAGC GC^T[ACGT]AGC
BsaAI 1438 CACGTG [CT]AC^GT[GA]
BsaHI 1229 GGCGCC G[GA]^CG[CT]C
BsmI 2511 GAATGC GAATGC(1/-1)
Bsp1407I 2432 TGTACA T^GTACA
BsrFI 1739 ACCGGC [GA]^CCGG[CT]
BsrGI 2432 TGTACA T^GTACA
BstUI 562 CGCG CG^CG
CelII 1398 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1739 ACCGGC [GA]^CCGG[CT]
DraIII 1997 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2647 GAATTC G^AATTC
HgaI 2553 GACGC GACGC(5/10)
Hin1I 1229 GGCGCC G[GA]^CG[CT]C
HincII 1330 GTTGAC GT[CT]^[GA]AC
HindII 1330 GTTGAC GT[CT]^[GA]AC
HindIII 987 AAGCTT A^AGCTT
KasI 1229 GGCGCC G^GCGCC
MluNI 1090 TGGCCA TGG^CCA
MscI 1090 TGGCCA TGG^CCA
MspA1I 392 CAGCTG C[AC]G^C[GT]G
MvnI 562 CGCG CG^CG
NarI 1229 GGCGCC GG^CGCC
NheI 2056 GCTAGC G^CTAGC
PmaCI 1438 CACGTG CAC^GTG
PmlI 1438 CACGTG CAC^GTG
PstI 2548 CTGCAG CTGCA^G
PvuII 392 CAGCTG CAG^CTG
ScaI 2585 AGTACT AGT^ACT
SfcI 2548 CTGCAG C^T[GA][CT]AG
SfoI 1229 GGCGCC GGC^GCC
SmlI 2404 CTCAAG C^T[CT][GA]AG
SphI 2698 GCATGC GCATG^C
SspBI 2432 TGTACA T^GTACA
StuI 2090 AGGCCT AGG^CCT
XmnI 771 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BanI 1229 GGCGCC G^G[CT][GA]CC
1423 GGCACC
BbsI 1776 GAAGAC GAAGAC(2/6)
2596 GAAGAC
BpuAI 1776 GAAGAC GAAGAC(2/6)
2596 GAAGAC
BspMI 2546 ACCTGC ACCTGC(4/8)
2370 GCAGGT
BsrDI 1275 CATTGC GCAATG(2/0)
386 GCAATG
DraI 1363 TTTAAA TTT^AAA
2565 TTTAAA
EaeI 1090 TGGCCA [CT]^GGCC[GA]
2459 TGGCCG
EcoNI 2133 CCTAACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2229 CCTCAATGAGG
EcoT14I 302 CCAAGG C^C[AT][AT]GG
2660 CCAAGG
HaeII 1055 GGCGCT [GA]GCGC^[CT]
1229 GGCGCC
MaeII 1439 ACGT A^CGT
2634 ACGT
NspI 2441 ACATGC [GA]CATG^[CT]
2698 GCATGC
StyI 302 CCAAGG C^C[AT][AT]GG
2660 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII