Restriction map for
pF1KSDA0997
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MroI MslI MspI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AcsI 799 GAATTT [GA]^AATT[CT]
AflII 1776 CTTAAG C^TTAAG
ApoI 799 GAATTT [GA]^AATT[CT]
Asp700I 994 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1299 GGACC G^G[AT]CC
BanII 1414 GAGCCC G[GA]GC[CT]^C
BbsI 340 GAAGAC GAAGAC(2/6)
BciVI 1559 GTATCC GTATCC(6/5)
BfrI 1776 CTTAAG C^TTAAG
BmyI 1414 GAGCCC G[AGT]GC[ACT]^C
BpmI 273 CTCCAG CTGGAG(16/14)
BpuAI 340 GAAGAC GAAGAC(2/6)
BsaJI 1292 CCGAGG C^C[ACGT][ACGT]GG
BsmAI 857 GAGAC GTCTC(1/5)
BsmFI 1535 GGGAC GGGAC(10/14)
Bsp1286I 1414 GAGCCC G[AGT]GC[ACT]^C
BspHI 158 TCATGA T^CATGA
BspLU11I 1828 ACATGT A^CATGT
EcoT22I 1259 ATGCAT ATGCA^T
MaeII 618 ACGT A^CGT
MfeI 86 CAATTG C^AATTG
MspA1I 1765 CAGCGG C[AC]G^C[GT]G
MunI 86 CAATTG C^AATTG
NdeI 1654 CATATG CA^TATG
NsiI 1259 ATGCAT ATGCA^T
PflMI 467 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1298 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 158 TCATGA T^CATGA
SapI 1096 GAAGAGC GCTCTTC(1/4)
ScaI 175 AGTACT AGT^ACT
SmlI 1776 CTTAAG C^T[CT][GA]AG
Van91I 467 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 994 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AciI 639 GCGG CCGC(-3/-1)
1767 GCGG
AflIII 618 ACGTGT A^C[GA][CT]GT
1828 ACATGT
BalI 1617 TGGCCA TGG^CCA
1892 TGGCCA
BglII 742 AGATCT A^GATCT
1422 AGATCT
BsrDI 298 GCAATG GCAATG(2/0)
458 GCAATG
BssKI 348 CCAGG ^CC[ACGT]GG
867 CCAGG
BstNI 348 CCAGG CC^[AT]GG
867 CCAGG
BstYI 742 AGATCT [GA]^GATC[CT]
1422 AGATCT
DraI 289 TTTAAA TTT^AAA
1579 TTTAAA
EaeI 1617 TGGCCA [CT]^GGCC[GA]
1892 TGGCCA
EcoRII 348 CCAGG ^CC[AT]GG
867 CCAGG
HgaI 629 GACGC GACGC(5/10)
1854 GCGTC
HphI 805 GGTGA GGTGA(8/7)
1900 GGTGA
MflI 742 AGATCT [GA]^GATC[CT]
1422 AGATCT
MluNI 1617 TGGCCA TGG^CCA
1892 TGGCCA
MscI 1617 TGGCCA TGG^CCA
1892 TGGCCA
MvaI 348 CCAGG CC^[AT]GG
867 CCAGG
NlaIV 987 GGAGCC GG[ACGT]^[ACGT]CC
1217 GGCCCC
PleI 532 GAGTC GAGTC(4/5)
922 GAGTC
ScrFI 348 CCAGG CC^[ACGT]GG
867 CCAGG
Tsp45I 71 GTGAC ^GT[GC]AC
1772 GTCAC
XhoII 742 AGATCT [GA]^GATC[CT]
1422 AGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AfaI AluI BbvI BfaI BseRI
BsgI BsiYI BslI BsrI BstF5I
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NspI RsaI Sau3AI
Sau96I SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I