Restriction map for
pF1KSDA0976
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AciI AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseRI BsgI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstXI CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI HinP1I HindIII HphI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 639 GTATAC GT^[AC][GT]AC
AccIII 201 TCCGGA T^CCGGA
AcyI 464 GGCGTC G[GA]^CG[CT]C
AflIII 257 ACATGT A^C[GA][CT]GT
AocI 783 CCTGAGG CC^T[ACGT]AGG
AspHI 952 GAGCAC G[AT]GC[AT]^C
BamHI 564 GGATCC G^GATCC
BbsI 332 GAAGAC GAAGAC(2/6)
BcgI 880 CGAGGAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 952 GAGCAC G[AGT]GC[ACT]^C
BpuAI 332 GAAGAC GAAGAC(2/6)
BsaHI 464 GGCGTC G[GA]^CG[CT]C
BsaI 218 GAGACC GGTCTC(1/5)
BsaWI 201 TCCGGA [AT]^CCGG[AT]
BseAI 201 TCCGGA T^CCGGA
BsiHKAI 952 GAGCAC G[AT]GC[AT]^C
BsmBI 229 GAGACG CGTCTC(1/5)
BsmFI 81 GGGAC GGGAC(10/14)
Bsp1286I 952 GAGCAC G[AGT]GC[ACT]^C
BspEI 201 TCCGGA T^CCGGA
BspLU11I 257 ACATGT A^CATGT
BsrDI 241 GCAATG GCAATG(2/0)
Bst1107I 639 GTATAC GTA^TAC
BstYI 564 GGATCC [GA]^GATC[CT]
BstZ17I 639 GTATAC GTA^TAC
Bsu36I 783 CCTGAGG CC^T[ACGT]AGG
DrdI 1007 GACCTTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 161 CCACGG C^C[GA][CT]GG
EarI 622 GAAGAG CTCTTC(1/4)
Eco81I 783 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1009 CCTTGG C^C[AT][AT]GG
HgaI 465 GCGTC GACGC(5/10)
Hin1I 464 GGCGTC G[GA]^CG[CT]C
HincII 394 GTTAAC GT[CT]^[GA]AC
HindII 394 GTTAAC GT[CT]^[GA]AC
HpaI 394 GTTAAC GTT^AAC
Ksp632I 622 GAAGAG CTCTTC(1/4)
MaeII 232 ACGT A^CGT
MflI 564 GGATCC [GA]^GATC[CT]
MroI 201 TCCGGA T^CCGGA
MspA1I 736 CAGCTG C[AC]G^C[GT]G
MwoI 688 GCGTTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PvuII 736 CAGCTG CAG^CTG
ScaI 626 AGTACT AGT^ACT
SfcI 962 CTACAG C^T[GA][CT]AG
SspI 819 AATATT AAT^ATT
StyI 1009 CCTTGG C^C[AT][AT]GG
Tsp45I 775 GTCAC ^GT[GC]AC
XhoII 564 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 687 CGCG CG^CG
858 CGCG
AfaI 284 GTAC GT^AC
627 GTAC
AvaII 700 GGACC G^G[AT]CC
967 GGTCC
BbvI 60 GCAGC GCAGC(8/12)
516 GCAGC
BpmI 388 CTCCAG CTGGAG(16/14)
484 CTGGAG
BsaJI 161 CCACGG C^C[ACGT][ACGT]GG
1009 CCTTGG
BsrI 1067 CCAGT ACTGG(1/-1)
1076 CCAGT
BstF5I 337 CATCC GGATG(2/0)
1035 CATCC
BstUI 687 CGCG CG^CG
858 CGCG
Fnu4HI 60 GCAGC GC^[ACGT]GC
516 GCAGC
FokI 337 CATCC GGATG(9/13)
1035 CATCC
HaeIII 144 GGCC GG^CC
1003 GGCC
HapII 154 CCGG C^CGG
202 CCGG
HpaII 154 CCGG C^CGG
202 CCGG
ItaI 60 GCAGC GC^[ACGT]GC
516 GCAGC
MaeIII 40 GTTAC ^GT[ACGT]AC
775 GTCAC
MseI 395 TTAA T^TAA
799 TTAA
MspI 154 CCGG C^CGG
202 CCGG
MvnI 687 CGCG CG^CG
858 CGCG
NlaIV 564 GGATCC GG[ACGT]^[ACGT]CC
1021 GGCTCC
NspI 257 ACATGT [GA]CATG^[CT]
940 ACATGC
PleI 102 GACTC GAGTC(4/5)
1014 GAGTC
RsaI 284 GTAC GT^AC
627 GTAC
Tru9I 395 TTAA T^TAA
799 TTAA
TseI 60 GCAGC G^C[AT]GC
516 GCAGC
Tsp509I 935 AATT ^AATT
994 AATT
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# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AlwI BciVI BsiYI BslI
BsmAI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII HinfI MboI
MboII MnlI MslI MvaI NdeII
NlaIII PflMI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI TspRI TthHB8I
Van91I