Restriction map for
pF1KSDA0969
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI AspEI AviII
AvrII BalI BbrPI BclI BfrI
BlnI BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MfeI MluI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 981 GGTACC G^GTACC
AccI 2611 GTAGAC GT^[AC][GT]AC
AccII 1798 CGCG CG^CG
AcyI 3115 GGCGCC G[GA]^CG[CT]C
AflIII 371 ACACGT A^C[GA][CT]GT
AlwNI 1708 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2821 CCTGAGG CC^T[ACGT]AGG
Asp700I 994 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 981 GGTACC G^GTACC
AspI 2764 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3115 GGCGCC GGCGC^C
BbsI 994 GAAGAC GAAGAC(2/6)
BcgI 119 GCAAAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 296 GTATCC GTATCC(6/5)
BglII 1952 AGATCT A^GATCT
BlpI 382 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC
BpuAI 994 GAAGAC GAAGAC(2/6)
BsaHI 3115 GGCGCC G[GA]^CG[CT]C
BsaI 1869 GGTCTC GGTCTC(1/5)
BsmBI 857 CGTCTC CGTCTC(1/5)
BssSI 2605 CACGAG CACGAG(-5/-1)
BstEII 2346 GGTGACC G^GT[ACGT]ACC
BstPI 2346 GGTGACC G^GT[ACGT]ACC
BstUI 1798 CGCG CG^CG
BstXI 801 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2821 CCTGAGG CC^T[ACGT]AGG
CelII 382 GCTGAGC GC^T[ACGT]AGC
EagI 1108 CGGCCG C^GGCCG
EclXI 1108 CGGCCG C^GGCCG
Eco52I 1108 CGGCCG C^GGCCG
Eco81I 2821 CCTGAGG CC^T[ACGT]AGG
EcoNI 3005 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2346 GGTGACC G^GT[ACGT]ACC
HgaI 95 GCGTC GACGC(5/10)
Hin1I 3115 GGCGCC G[GA]^CG[CT]C
KasI 3115 GGCGCC G^GCGCC
KpnI 981 GGTACC GGTAC^C
MvnI 1798 CGCG CG^CG
NarI 3115 GGCGCC GG^CGCC
NcoI 455 CCATGG C^CATGG
PflFI 2764 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2554 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 195 GCTCTTC GCTCTTC(1/4)
SfoI 3115 GGCGCC GGC^GCC
SgrAI 883 CACCGGCG C[GA]^CCGG[CT]G
Tth111I 2764 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 53 CCAACCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 994 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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Aor51HI 140 AGCGCT AGC^GCT
236 AGCGCT
ApaI 765 GGGCCC GGGCC^C
1160 GGGCCC
BsaBI 1078 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2973 GATTGAAATC
BsaWI 696 TCCGGT [AT]^CCGG[AT]
726 ACCGGA
BsiEI 1108 CGGCCG CG[GA][CT]^CG
2996 CGACCG
BsmI 2373 GAATGC GAATGC(1/-1)
2480 GAATGC
DraIII 1360 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1427 CACCCAGTG
Eco47III 140 AGCGCT AGC^GCT
236 AGCGCT
MamI 1078 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2973 GATTGAAATC
NaeI 833 GCCGGC GCC^GGC
1131 GCCGGC
NgoMIV 833 GCCGGC G^CCGGC
1131 GCCGGC
SacI 936 GAGCTC GAGCT^C
2749 GAGCTC
ScaI 1046 AGTACT AGT^ACT
1088 AGTACT
SfiI 665 GGCCTGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2538 GGCCAGCCCGGCC
SmaI 2003 CCCGGG CCC^GGG
2678 CCCGGG
XmaCI 2003 CCCGGG C^CCGGG
2678 CCCGGG
XmaI 2003 CCCGGG C^CCGGG
2678 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII