Restriction map for
pF1KSDA0965
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KspI MaeI MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1041 GGTACC G^GTACC
AccI 1156 GTCGAC GT^[AC][GT]AC
AciI 312 GCGG CCGC(-3/-1)
AlwI 609 GATCC GGATC(4/5)
AlwNI 584 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1041 GGTACC G^GTACC
AspHI 1165 GAGCAC G[AT]GC[AT]^C
AvaI 50 CCCGGG C^[CT]CG[GA]G
BalI 152 TGGCCA TGG^CCA
BbvI 1186 GCAGC GCAGC(8/12)
BmyI 1165 GAGCAC G[AGT]GC[ACT]^C
BsaWI 1284 ACCGGA [AT]^CCGG[AT]
BseRI 293 GAGGAG GAGGAG(10/8)
BsiHKAI 1165 GAGCAC G[AT]GC[AT]^C
BsoBI 50 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1165 GAGCAC G[AGT]GC[ACT]^C
BspMI 390 GCAGGT ACCTGC(4/8)
DraIII 761 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 155 CCATGG C^C[GA][CT]GG
EaeI 152 TGGCCA [CT]^GGCC[GA]
EcoNI 1325 CCTATAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 956 GATATC GAT^ATC
Fnu4HI 1186 GCAGC GC^[ACGT]GC
ItaI 1186 GCAGC GC^[ACGT]GC
KpnI 1041 GGTACC GGTAC^C
MluNI 152 TGGCCA TGG^CCA
MscI 152 TGGCCA TGG^CCA
NcoI 155 CCATGG C^CATGG
SalI 1156 GTCGAC G^TCGAC
SmaI 50 CCCGGG CCC^GGG
SmlI 983 CTCAAG C^T[CT][GA]AG
TseI 1186 GCAGC G^C[AT]GC
TspRI 1265 CAGTG CA[GC]TG[ACGT][ACGT]^
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 704 GTAC GT^AC
1042 GTAC
AhdI 265 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
913 GACTGGTGGTC
AspEI 265 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
913 GACTGGTGGTC
AvaII 245 GGACC G^G[AT]CC
318 GGTCC
BanI 439 GGTGCC G^G[CT][GA]CC
1041 GGTACC
BcnI 50 CCCGG CC^[GC]GG
51 CCGGG
BsmFI 16 GGGAC GGGAC(10/14)
853 GGGAC
BstAPI 433 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
598 GCAATAGATGC
Cac8I 211 GCTCGC GC[ACGT]^[ACGT]GC
1182 GCCAGC
DdeI 1052 CTGAG C^T[ACGT]AG
1075 CTCAG
DpnII 200 GATC ^GATC
609 GATC
Eam1105I 265 GACTTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
913 GACTGGTGGTC
HapII 51 CCGG C^CGG
1285 CCGG
HincII 1156 GTCGAC GT[CT]^[GA]AC
1344 GTTGAC
HindII 1156 GTCGAC GT[CT]^[GA]AC
1344 GTTGAC
HpaII 51 CCGG C^CGG
1285 CCGG
HphI 450 GGTGA GGTGA(8/7)
517 GGTGA
MaeII 195 ACGT A^CGT
1353 ACGT
MboI 200 GATC ^GATC
609 GATC
MslI 340 CATATCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1368 CACCTACATG
MspI 51 CCGG C^CGG
1285 CCGG
NciI 50 CCCGG CC^[GC]GG
51 CCGGG
NdeII 200 GATC ^GATC
609 GATC
NlaIV 439 GGTGCC GG[ACGT]^[ACGT]CC
1041 GGTACC
PflMI 612 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1298 CCAAAGACTGG
PleI 1347 GACTC GAGTC(4/5)
271 GAGTC
RsaI 704 GTAC GT^AC
1042 GTAC
Sau3AI 200 GATC ^GATC
609 GATC
SfaNI 604 GATGC GCATC(5/9)
664 GATGC
SfcI 65 CTGTAG C^T[GA][CT]AG
1196 CTATAG
TaqI 198 TCGA T^CGA
1157 TCGA
TthHB8I 198 TCGA T^CGA
1157 TCGA
Van91I 612 CCACCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1298 CCAAAGACTGG
XcmI 71 CCAAGCTCACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
248 CCAGACTTGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI ApoI BpmI BsaJI
BsiYI BslI BsmAI BsrDI BsrI
BssKI BstF5I BstNI Cfr13I EarI
EcoRII EcoT14I FokI HaeIII HinfI
Ksp632I MaeIII MboII MnlI MseI
MvaI MwoI NlaIII Sau96I ScrFI
StyI TfiI Tru9I Tsp45I Tsp509I