Restriction map for
pF1KSDA0961
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsoBI BspDI
BspEI BspMI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SapI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 1011 GCGG CCGC(-3/-1)
AflIII 670 ACATGT A^C[GA][CT]GT
AhdI 1083 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 865 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 1083 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 190 GAGCCC G[GA]GC[CT]^C
BbvI 1014 GCAGC GCAGC(8/12)
BclI 164 TGATCA T^GATCA
BfaI 232 CTAG C^TAG
BglII 662 AGATCT A^GATCT
BpmI 962 CTGGAG CTGGAG(16/14)
BsaJI 193 CCCTGG C^C[ACGT][ACGT]GG
BsmAI 867 GAGAC GTCTC(1/5)
BsmI 61 GAATGC GAATGC(1/-1)
Bsp1407I 89 TGTACA T^GTACA
BspHI 360 TCATGA T^CATGA
BspLU11I 670 ACATGT A^CATGT
BsrDI 876 CATTGC GCAATG(2/0)
BsrGI 89 TGTACA T^GTACA
BssKI 194 CCTGG ^CC[ACGT]GG
BssSI 5 CTCGTG CACGAG(-5/-1)
BstNI 194 CCTGG CC^[AT]GG
BstYI 662 AGATCT [GA]^GATC[CT]
Eam1105I 1083 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 431 CTCTTC CTCTTC(1/4)
EcoRII 194 CCTGG ^CC[AT]GG
FbaI 164 TGATCA T^GATCA
HincII 605 GTCGAC GT[CT]^[GA]AC
HindII 605 GTCGAC GT[CT]^[GA]AC
Ksp632I 431 CTCTTC CTCTTC(1/4)
MaeI 232 CTAG C^TAG
MflI 662 AGATCT [GA]^GATC[CT]
MvaI 194 CCTGG CC^[AT]GG
NspI 670 ACATGT [GA]CATG^[CT]
PleI 228 GACTC GAGTC(4/5)
RcaI 360 TCATGA T^CATGA
SalI 605 GTCGAC G^TCGAC
ScrFI 194 CCTGG CC^[ACGT]GG
SspBI 89 TGTACA T^GTACA
TseI 1014 GCAGC G^C[AT]GC
XbaI 231 TCTAGA T^CTAGA
XhoII 662 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 37 GTAGAC GT^[AC][GT]AC
605 GTCGAC
AluI 331 AGCT AG^CT
1271 AGCT
Asp700I 1080 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1457 GAATTCATTC
BbsI 1080 GAAGAC GAAGAC(2/6)
1332 GAAGAC
BmyI 190 GAGCCC G[AGT]GC[ACT]^C
593 GTGCCC
BpuAI 1080 GAAGAC GAAGAC(2/6)
1332 GAAGAC
Bsp1286I 190 GAGCCC G[AGT]GC[ACT]^C
593 GTGCCC
DpnII 165 GATC ^GATC
663 GATC
Fnu4HI 1011 GCGGC GC^[ACGT]GC
1014 GCAGC
ItaI 1011 GCGGC GC^[ACGT]GC
1014 GCAGC
MboI 165 GATC ^GATC
663 GATC
NdeII 165 GATC ^GATC
663 GATC
NlaIII 361 CATG CATG^
671 CATG
Sau3AI 165 GATC ^GATC
663 GATC
SfcI 35 CTGTAG C^T[GA][CT]AG
114 CTATAG
Tsp45I 129 GTCAC ^GT[GC]AC
382 GTGAC
XmnI 1080 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1457 GAATTCATTC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AfaI ApoI BsrI BstF5I
DdeI EcoRI FokI HaeIII HinfI
HphI MaeIII MboII MnlI MseI
MslI RsaI StuI TaqI TfiI
Tru9I Tsp509I TspRI TthHB8I