Restriction map for
pF1KSDA0941
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BalI BamHI BanII BbeI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KspI MaeI MluI
MluNI MroI MscI MslI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 94 GGTACC G^GTACC
AciI 443 CCGC CCGC(-3/-1)
AlwI 1135 GGATC GGATC(4/5)
AlwNI 1372 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 94 GGTACC G^GTACC
AspHI 55 GTGCTC G[AT]GC[AT]^C
AvaI 1429 CTCGAG C^[CT]CG[GA]G
BbrPI 40 CACGTG CAC^GTG
BbvCI 655 CCTCAGC CCTCAGC(-5/-2)
BmyI 55 GTGCTC G[AGT]GC[ACT]^C
BsaAI 40 CACGTG [CT]AC^GT[GA]
BsaBI 362 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 184 GAGGAG GAGGAG(10/8)
BsgI 43 GTGCAG GTGCAG(16/14)
BsiHKAI 55 GTGCTC G[AT]GC[AT]^C
BsmAI 800 GTCTC GTCTC(1/5)
BsmI 675 GCATTC GAATGC(1/-1)
BsoBI 1429 CTCGAG C^[CT]CG[GA]G
Bsp1286I 55 GTGCTC G[AGT]GC[ACT]^C
BsrDI 1286 GCAATG GCAATG(2/0)
CfoI 673 GCGC GCG^C
EarI 1360 GAAGAG CTCTTC(1/4)
EcoT14I 955 CCAAGG C^C[AT][AT]GG
EcoT22I 1169 ATGCAT ATGCA^T
HaeIII 189 GGCC GG^CC
HhaI 673 GCGC GCG^C
HinP1I 673 GCGC G^CGC
HincII 1209 GTCAAC GT[CT]^[GA]AC
HindII 1209 GTCAAC GT[CT]^[GA]AC
HindIII 828 AAGCTT A^AGCTT
KpnI 94 GGTACC GGTAC^C
Ksp632I 1360 GAAGAG CTCTTC(1/4)
MamI 362 GATTAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1025 CAATTG C^AATTG
MspA1I 121 CAGCTG C[AC]G^C[GT]G
MunI 1025 CAATTG C^AATTG
MwoI 6 GCTGTCCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 1169 ATGCAT ATGCA^T
NspI 575 ACATGC [GA]CATG^[CT]
PaeR7I 1429 CTCGAG C^TCGAG
PflMI 787 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 40 CACGTG CAC^GTG
PmlI 40 CACGTG CAC^GTG
PshAI 662 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1334 CTGCAG CTGCA^G
PvuII 121 CAGCTG CAG^CTG
SspI 415 AATATT AAT^ATT
StuI 188 AGGCCT AGG^CCT
StyI 955 CCAAGG C^C[AT][AT]GG
Van91I 787 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1429 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 1296 CCTCAGG CC^T[ACGT]AGG
1398 CCTTAGG
Asp700I 533 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1360 GAAGAGGTTC
BanI 94 GGTACC G^G[CT][GA]CC
730 GGCACC
BbvI 983 GCAGC GCAGC(8/12)
1001 GCAGC
BcnI 1308 CCCGG CC^[GC]GG
1422 CCGGG
BsaJI 269 CCCTGG C^C[ACGT][ACGT]GG
955 CCAAGG
BspMI 45 GCAGGT ACCTGC(4/8)
297 GCAGGT
Bsu36I 1296 CCTCAGG CC^T[ACGT]AGG
1398 CCTTAGG
Eco81I 1296 CCTCAGG CC^T[ACGT]AGG
1398 CCTTAGG
Fnu4HI 983 GCAGC GC^[ACGT]GC
1001 GCAGC
HapII 1309 CCGG C^CGG
1422 CCGG
HpaII 1309 CCGG C^CGG
1422 CCGG
ItaI 983 GCAGC GC^[ACGT]GC
1001 GCAGC
MaeII 41 ACGT A^CGT
547 ACGT
MspI 1309 CCGG C^CGG
1422 CCGG
NciI 1308 CCCGG CC^[GC]GG
1422 CCGGG
ScaI 137 AGTACT AGT^ACT
568 AGTACT
SmlI 166 CTTGAG C^T[CT][GA]AG
1429 CTCGAG
TseI 983 GCAGC G^C[AT]GC
1001 GCAGC
XmnI 533 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1360 GAAGAGGTTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BglII BpuAI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FokI HinfI
HphI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoII