Restriction map for
pF1KSDA0934
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AflII AgeI AseI AsnI
Asp700I AviII AvrII BfrI BlnI
BsiWI Bsp1407I BspDI BspLU11I BsrFI
BsrGI BstBI Cfr10I ClaI DraIII
EagI EclXI Eco52I EcoRV FseI
FspI HincII HindII HpaI MfeI
MluI MunI NaeI NdeI NgoMIV
NotI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI SspI
SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 4358 GGTACC G^GTACC
AccIII 1985 TCCGGA T^CCGGA
AhdI 1931 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3714 GTGCAC G^TGCAC
AocI 3929 CCTCAGG CC^T[ACGT]AGG
Aor51HI 254 AGCGCT AGC^GCT
ApaI 896 GGGCCC GGGCC^C
ApaLI 3714 GTGCAC G^TGCAC
AscI 48 GGCGCGCC GG^CGCGCC
Asp718I 4358 GGTACC G^GTACC
AspEI 1931 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3982 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 999 GGCGCC GGCGC^C
BbrPI 3085 CACGTG CAC^GTG
BbvCI 2164 CCTCAGC CCTCAGC(-5/-2)
BciVI 3336 GGATAC GTATCC(6/5)
BclI 1685 TGATCA T^GATCA
BglII 3371 AGATCT A^GATCT
BlpI 2530 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2530 GCTGAGC GC^T[ACGT]AGC
BseAI 1985 TCCGGA T^CCGGA
BsiEI 799 CGACCG CG[GA][CT]^CG
BspEI 1985 TCCGGA T^CCGGA
BspHI 1667 TCATGA T^CATGA
BsrDI 2729 GCAATG GCAATG(2/0)
BssHII 49 GCGCGC G^CGCGC
Bst1107I 2603 GTATAC GTA^TAC
BstZ17I 2603 GTATAC GTA^TAC
Bsu36I 3929 CCTCAGG CC^T[ACGT]AGG
CelII 2530 GCTGAGC GC^T[ACGT]AGC
CpoI 5 CGGACCG CG^G[AT]CCG
DraI 1378 TTTAAA TTT^AAA
Eam1105I 1931 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 254 AGCGCT AGC^GCT
Eco81I 3929 CCTCAGG CC^T[ACGT]AGG
EcoRI 823 GAATTC G^AATTC
FbaI 1685 TGATCA T^GATCA
HindIII 167 AAGCTT A^AGCTT
KasI 999 GGCGCC G^GCGCC
KpnI 4358 GGTACC GGTAC^C
KspI 3868 CCGCGG CCGC^GG
MroI 1985 TCCGGA T^CCGGA
NarI 999 GGCGCC GG^CGCC
NheI 3438 GCTAGC G^CTAGC
NruI 3194 TCGCGA TCG^CGA
PflFI 3982 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3085 CACGTG CAC^GTG
PmlI 3085 CACGTG CAC^GTG
RcaI 1667 TCATGA T^CATGA
RsrII 5 CGGACCG CG^G[AT]CCG
SacII 3868 CCGCGG CCGC^GG
SfoI 999 GGCGCC GGC^GCC
SmaI 2016 CCCGGG CCC^GGG
SpeI 1321 ACTAGT A^CTAGT
Tth111I 3982 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3517 TCTAGA T^CTAGA
XmaCI 2016 CCCGGG C^CCGGG
XmaI 2016 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 823 GAATTC [GA]^AATT[CT]
4437 AAATTT
AflIII 1501 ACGTGT A^C[GA][CT]GT
3683 ACACGT
ApoI 823 GAATTC [GA]^AATT[CT]
4437 AAATTT
BsaBI 1686 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2517 GATCGTGATC
BsaWI 867 ACCGGA [AT]^CCGG[AT]
1985 TCCGGA
BsmBI 4169 GAGACG CGTCTC(1/5)
4616 GAGACG
BsmI 1844 GAATGC GAATGC(1/-1)
1094 GCATTC
BsrBI 313 GAGCGG CCGCTC(-3/-3)
546 GAGCGG
BssSI 245 CACGAG CACGAG(-5/-1)
2116 CTCGTG
BstAPI 3377 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4599 GCAGCGCATGC
BstEII 2493 GGTGACC G^GT[ACGT]ACC
4503 GGTGACC
BstPI 2493 GGTGACC G^GT[ACGT]ACC
4503 GGTGACC
DrdI 2217 GACGGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4561 GACATCGGCGTC
EcoO65I 2493 GGTGACC G^GT[ACGT]ACC
4503 GGTGACC
MamI 1686 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2517 GATCGTGATC
PflMI 567 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGATGCTGG
SacI 718 GAGCTC GAGCT^C
4273 GAGCTC
SexAI 2825 ACCTGGT A^CC[AT]GGT
4490 ACCTGGT
SmlI 3748 CTCAAG C^T[CT][GA]AG
4200 CTCAAG
SphI 4352 GCATGC GCATG^C
4604 GCATGC
Van91I 567 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
900 CCAGATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII