Restriction map for
pF1KSDA0895
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BcgI BciVI BcnI
BglI BlnI BmyI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstXI Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII HphI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI SspBI SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1174 GTATAC GT^[AC][GT]AC
AccII 16 CGCG CG^CG
AciI 354 GCGG CCGC(-3/-1)
AflII 706 CTTAAG C^TTAAG
BbrPI 806 CACGTG CAC^GTG
BbsI 222 GTCTTC GAAGAC(2/6)
BclI 89 TGATCA T^GATCA
BfrI 706 CTTAAG C^TTAAG
BglII 1398 AGATCT A^GATCT
BlpI 676 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 676 GCTAAGC GC^T[ACGT]AGC
BpuAI 222 GTCTTC GAAGAC(2/6)
BsaAI 806 CACGTG [CT]AC^GT[GA]
BsaBI 1264 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1009 CTCCTC GAGGAG(10/8)
BsgI 803 CTGCAC GTGCAG(16/14)
BsmFI 1309 GTCCC GGGAC(10/14)
Bst1107I 1174 GTATAC GTA^TAC
BstUI 16 CGCG CG^CG
BstZ17I 1174 GTATAC GTA^TAC
CelII 676 GCTAAGC GC^T[ACGT]AGC
DraI 1060 TTTAAA TTT^AAA
EaeI 1332 TGGCCG [CT]^GGCC[GA]
EarI 485 CTCTTC CTCTTC(1/4)
EcoRI 1187 GAATTC G^AATTC
EcoRV 409 GATATC GAT^ATC
FbaI 89 TGATCA T^GATCA
HgaI 751 GCGTC GACGC(5/10)
Ksp632I 485 CTCTTC CTCTTC(1/4)
MamI 1264 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 505 CAGCTG C[AC]G^C[GT]G
MvnI 16 CGCG CG^CG
PmaCI 806 CACGTG CAC^GTG
PmlI 806 CACGTG CAC^GTG
PstI 1321 CTGCAG CTGCA^G
PvuII 505 CAGCTG CAG^CTG
SbfI 1320 CCTGCAGG CCTGCA^GG
ScaI 435 AGTACT AGT^ACT
SexAI 1169 ACCAGGT A^CC[AT]GGT
SfaNI 691 GCATC GCATC(5/9)
SmlI 706 CTTAAG C^T[CT][GA]AG
SpeI 373 ACTAGT A^CTAGT
Sse8387I 1320 CCTGCAGG CCTGCA^GG
TaqI 861 TCGA T^CGA
TthHB8I 861 TCGA T^CGA
XbaI 511 TCTAGA T^CTAGA
XcmI 886 CCATGGAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 550 AAATTT [GA]^AATT[CT]
1187 GAATTC
AlwI 289 GATCC GGATC(4/5)
1269 GGATC
ApoI 550 AAATTT [GA]^AATT[CT]
1187 GAATTC
Asp700I 25 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
107 GAAGCATTTC
BanI 312 GGTGCC G^G[CT][GA]CC
470 GGTGCC
BbvCI 502 CCTCAGC CCTCAGC(-5/-2)
831 GCTGAGG
BbvI 882 GCAGC GCAGC(8/12)
1003 GCTGC
BsiYI 1145 CCCAACCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1335 CCGATATATGG
BslI 1145 CCCAACCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1335 CCGATATATGG
BsmI 827 GAATGC GAATGC(1/-1)
959 GCATTC
BspMI 737 ACCTGC ACCTGC(4/8)
1073 GCAGGT
BstYI 288 AGATCC [GA]^GATC[CT]
1398 AGATCT
DsaI 886 CCATGG C^C[GA][CT]GG
1329 CCATGG
EcoT14I 886 CCATGG C^C[AT][AT]GG
1329 CCATGG
HindIII 28 AAGCTT A^AGCTT
516 AAGCTT
MaeII 807 ACGT A^CGT
906 ACGT
MflI 288 AGATCC [GA]^GATC[CT]
1398 AGATCT
MslI 12 CATTCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1362 CATGGAAGTG
MwoI 30 GCTTCACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
649 GCCAATCCTGC
NcoI 886 CCATGG C^CATGG
1329 CCATGG
PleI 780 GACTC GAGTC(4/5)
7 GAGTC
SfcI 181 CTACAG C^T[GA][CT]AG
1321 CTGCAG
StyI 886 CCATGG C^C[AT][AT]GG
1329 CCATGG
TfiI 82 GATTC G^A[AT]TC
605 GATTC
TseI 882 GCAGC G^C[AT]GC
1003 GCTGC
Tsp509I 551 AATT ^AATT
1188 AATT
XhoII 288 AGATCC [GA]^GATC[CT]
1398 AGATCT
XmnI 25 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
107 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AvaI AvaII BfaI
BpmI BsaJI BsoBI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI Tru9I
Tsp45I TspRI