Restriction map for
pF1KSDA0888
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII AccII AccIII AclI AcyI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BcnI
BglI BglII BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstXI ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 743 GGTACC G^GTACC
AflIII 68 ACATGT A^C[GA][CT]GT
AgeI 1662 ACCGGT A^CCGGT
AhdI 883 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1441 CCTGAGG CC^T[ACGT]AGG
Asp718I 743 GGTACC G^GTACC
AspEI 883 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 743 GGTACC G^G[CT][GA]CC
BclI 252 TGATCA T^GATCA
BlpI 1999 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1999 GCTAAGC GC^T[ACGT]AGC
BsaAI 1469 CACGTA [CT]AC^GT[GA]
BsaWI 1662 ACCGGT [AT]^CCGG[AT]
BsgI 1115 CTGCAC GTGCAG(16/14)
BspHI 954 TCATGA T^CATGA
BspLU11I 68 ACATGT A^CATGT
BsrFI 1662 ACCGGT [GA]^CCGG[CT]
BstYI 398 AGATCC [GA]^GATC[CT]
Bsu36I 1441 CCTGAGG CC^T[ACGT]AGG
CelII 1999 GCTAAGC GC^T[ACGT]AGC
CfoI 628 GCGC GCG^C
Cfr10I 1662 ACCGGT [GA]^CCGG[CT]
Eam1105I 883 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1441 CCTGAGG CC^T[ACGT]AGG
EcoRI 1141 GAATTC G^AATTC
FbaI 252 TGATCA T^GATCA
HapII 1663 CCGG C^CGG
HhaI 628 GCGC GCG^C
HinP1I 628 GCGC G^CGC
HpaII 1663 CCGG C^CGG
KpnI 743 GGTACC GGTAC^C
MfeI 526 CAATTG C^AATTG
MflI 398 AGATCC [GA]^GATC[CT]
MslI 404 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 230 CAGCTG C[AC]G^C[GT]G
MspI 1663 CCGG C^CGG
MunI 526 CAATTG C^AATTG
NdeI 495 CATATG CA^TATG
PflMI 1006 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1662 ACCGGT A^CCGGT
PshAI 824 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 230 CAGCTG CAG^CTG
RcaI 954 TCATGA T^CATGA
SacI 1131 GAGCTC GAGCT^C
SapI 1753 GCTCTTC GCTCTTC(1/4)
ScaI 421 AGTACT AGT^ACT
SfcI 485 CTACAG C^T[GA][CT]AG
SpeI 1342 ACTAGT A^CTAGT
SspI 124 AATATT AAT^ATT
Tsp45I 760 GTCAC ^GT[GC]AC
Van91I 1006 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 398 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 310 CTTAAG C^TTAAG
1306 CTTAAG
AlwI 399 GATCC GGATC(4/5)
1866 GGATC
AspHI 321 GAGCAC G[AT]GC[AT]^C
1131 GAGCTC
BanII 1131 GAGCTC G[GA]GC[CT]^C
1732 GAGCCC
BciVI 642 GTATCC GTATCC(6/5)
693 GTATCC
BfrI 310 CTTAAG C^TTAAG
1306 CTTAAG
BpmI 595 CTGGAG CTGGAG(16/14)
833 CTGGAG
BsaI 701 GAGACC GGTCTC(1/5)
1665 GGTCTC
BsaJI 1710 CCAGGG C^C[ACGT][ACGT]GG
1736 CCCAGG
BseRI 1717 GAGGAG GAGGAG(10/8)
1834 GAGGAG
BsiHKAI 321 GAGCAC G[AT]GC[AT]^C
1131 GAGCTC
BsmFI 89 GGGAC GGGAC(10/14)
1542 GTCCC
BsmI 1809 GAATGC GAATGC(1/-1)
4 GCATTC
BspMI 1983 ACCTGC ACCTGC(4/8)
315 GCAGGT
Bst1107I 663 GTATAC GTA^TAC
1472 GTATAC
BstF5I 1185 GGATG GGATG(2/0)
1497 GGATG
BstZ17I 663 GTATAC GTA^TAC
1472 GTATAC
DraII 89 GGGACCC [GA]G^G[ACGT]CC[CT]
1980 AGGACCT
EaeI 1003 CGGCCA [CT]^GGCC[GA]
1210 CGGCCA
EcoO109I 89 GGGACCC [GA]G^G[ACGT]CC[CT]
1980 AGGACCT
FokI 1185 GGATG GGATG(9/13)
1497 GGATG
MaeII 1470 ACGT A^CGT
1793 ACGT
MaeIII 521 GTTAC ^GT[ACGT]AC
760 GTCAC
NspI 68 ACATGT [GA]CATG^[CT]
590 ACATGC
PpuMI 89 GGGACCC [GA]G^G[AT]CC[CT]
1980 AGGACCT
TaqI 360 TCGA T^CGA
984 TCGA
TthHB8I 360 TCGA T^CGA
984 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AvaII BbsI BbvI BfaI
BmyI BpuAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI Tru9I
TseI Tsp509I TspRI