Restriction map for
pF1KSDA0885
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII Cfr10I ClaI DraI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoT22I FbaI FseI
Hin1I HindIII KasI Ksp632I KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 44 GGTACC G^GTACC
AciI 552 CCGC CCGC(-3/-1)
AclI 445 AACGTT AA^CGTT
AlwNI 2190 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1812 CCTGAGG CC^T[ACGT]AGG
ApaI 2220 GGGCCC GGGCC^C
Asp718I 44 GGTACC G^GTACC
AspI 1678 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2018 TGCGCA TGC^GCA
BalI 1916 TGGCCA TGG^CCA
BanI 44 GGTACC G^G[CT][GA]CC
BanII 2220 GGGCCC G[GA]GC[CT]^C
BbvCI 1360 GCTGAGG CCTCAGC(-5/-2)
BsmAI 899 GAGAC GTCTC(1/5)
BspHI 1611 TCATGA T^CATGA
Bsu36I 1812 CCTGAGG CC^T[ACGT]AGG
CpoI 224 CGGACCG CG^G[AT]CCG
DrdI 1523 GACTTTTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1916 TGGCCA [CT]^GGCC[GA]
Eco81I 1812 CCTGAGG CC^T[ACGT]AGG
EcoNI 1447 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 688 GAATTC G^AATTC
EcoRV 769 GATATC GAT^ATC
FauI 551 CCCGC CCCGC(4/6)
FspI 2018 TGCGCA TGC^GCA
HaeII 2191 AGCGCC [GA]GCGC^[CT]
HapII 358 CCGG C^CGG
HpaI 99 GTTAAC GTT^AAC
HpaII 358 CCGG C^CGG
KpnI 44 GGTACC GGTAC^C
MfeI 1963 CAATTG C^AATTG
MluNI 1916 TGGCCA TGG^CCA
MscI 1916 TGGCCA TGG^CCA
MspI 358 CCGG C^CGG
MunI 1963 CAATTG C^AATTG
PflFI 1678 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 445 AACGTT AA^CGTT
PstI 1936 CTGCAG CTGCA^G
RcaI 1611 TCATGA T^CATGA
RsrII 224 CGGACCG CG^G[AT]CCG
SmaI 357 CCCGGG CCC^GGG
SmlI 746 CTCAAG C^T[CT][GA]AG
SspI 955 AATATT AAT^ATT
StuI 1492 AGGCCT AGG^CCT
TfiI 2163 GATTC G^A[AT]TC
Tth111I 1678 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1547 CCAAGAGGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 357 CCCGGG C^CCGGG
XmaI 357 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 515 GTGCTC G[AT]GC[AT]^C
2297 GTGCTC
AvaI 357 CCCGGG C^[CT]CG[GA]G
1040 CCCGAG
AvaII 225 GGACC G^G[AT]CC
2332 GGACC
BcnI 357 CCCGG CC^[GC]GG
358 CCGGG
BfaI 140 CTAG C^TAG
1331 CTAG
BpmI 347 CTCCAG CTGGAG(16/14)
461 CTGGAG
BsiHKAI 515 GTGCTC G[AT]GC[AT]^C
2297 GTGCTC
BsmFI 1676 GGGAC GGGAC(10/14)
2331 GGGAC
BsoBI 357 CCCGGG C^[CT]CG[GA]G
1040 CCCGAG
BspMI 176 ACCTGC ACCTGC(4/8)
1503 GCAGGT
BsrDI 246 CATTGC GCAATG(2/0)
845 CATTGC
BstEII 921 GGTGACC G^GT[ACGT]ACC
937 GGTGACC
BstPI 921 GGTGACC G^GT[ACGT]ACC
937 GGTGACC
BstXI 24 CCACAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1421 CCAAGGATGTGG
BstYI 1435 GGATCT [GA]^GATC[CT]
2369 AGATCC
DraII 2219 AGGGCCC [GA]G^G[ACGT]CC[CT]
2220 GGGCCCC
DraIII 68 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1747 CACTCAGTG
EcoO109I 2219 AGGGCCC [GA]G^G[ACGT]CC[CT]
2220 GGGCCCC
EcoO65I 921 GGTGACC G^GT[ACGT]ACC
937 GGTGACC
HgaI 854 GACGC GACGC(5/10)
1952 GCGTC
HincII 99 GTTAAC GT[CT]^[GA]AC
865 GTTGAC
HindII 99 GTTAAC GT[CT]^[GA]AC
865 GTTGAC
MaeI 140 CTAG C^TAG
1331 CTAG
MflI 1435 GGATCT [GA]^GATC[CT]
2369 AGATCC
NciI 357 CCCGG CC^[GC]GG
358 CCGGG
PleI 366 GAGTC GAGTC(4/5)
2210 GAGTC
PvuII 350 CAGCTG CAG^CTG
451 CAGCTG
XhoII 1435 GGATCT [GA]^GATC[CT]
2369 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BmyI BsaJI BseRI BsgI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I