Restriction map for
pF1KSDA0876
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# NO CUT ENZYMES ( 62 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI BbvCI BfrI BsiWI
BsmBI BsmI BspDI BspLU11I BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoO65I EcoRV
EcoT22I HindIII HpaI KpnI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 461 TCCGGA T^CCGGA
Alw44I 918 GTGCAC G^TGCAC
ApaI 1470 GGGCCC GGGCC^C
ApaLI 918 GTGCAC G^TGCAC
Asp700I 1368 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2760 CCTAGG C^CTAGG
BcgI 497 GCACCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2597 GTATCC GTATCC(6/5)
BclI 806 TGATCA T^GATCA
BglII 944 AGATCT A^GATCT
BlnI 2760 CCTAGG C^CTAGG
BlpI 1062 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1062 GCTGAGC GC^T[ACGT]AGC
BsaBI 1812 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 461 TCCGGA T^CCGGA
Bsp1407I 584 TGTACA T^GTACA
BspEI 461 TCCGGA T^CCGGA
BsrGI 584 TGTACA T^GTACA
BssSI 2898 CACGAG CACGAG(-5/-1)
BstXI 275 CCATGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1062 GCTGAGC GC^T[ACGT]AGC
DraI 73 TTTAAA TTT^AAA
DrdI 228 GACGGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 799 GAATTC G^AATTC
FbaI 806 TGATCA T^GATCA
HincII 1246 GTTGAC GT[CT]^[GA]AC
HindII 1246 GTTGAC GT[CT]^[GA]AC
MamI 1812 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 461 TCCGGA T^CCGGA
NspI 539 GCATGT [GA]CATG^[CT]
ScaI 317 AGTACT AGT^ACT
SexAI 3017 ACCAGGT A^CC[AT]GGT
SfiI 232 GGCCAGTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 584 TGTACA T^GTACA
TfiI 862 GAATC G^A[AT]TC
XmnI 1368 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 70 GAATTT [GA]^AATT[CT]
799 GAATTC
AocI 1531 CCTGAGG CC^T[ACGT]AGG
2745 CCTGAGG
Aor51HI 983 AGCGCT AGC^GCT
1194 AGCGCT
ApoI 70 GAATTT [GA]^AATT[CT]
799 GAATTC
AspI 934 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2908 GACTGTGTC
AviII 856 TGCGCA TGC^GCA
965 TGCGCA
BamHI 764 GGATCC G^GATCC
2444 GGATCC
BglI 118 GCCCACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
233 GCCAGTCGGGC
BspHI 44 TCATGA T^CATGA
803 TCATGA
BssHII 2032 GCGCGC G^CGCGC
2479 GCGCGC
Bsu36I 1531 CCTGAGG CC^T[ACGT]AGG
2745 CCTGAGG
DraIII 912 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2506 CACCCTGTG
Eco47III 983 AGCGCT AGC^GCT
1194 AGCGCT
Eco81I 1531 CCTGAGG CC^T[ACGT]AGG
2745 CCTGAGG
FseI 1846 GGCCGGCC GGCCGG^CC
3268 GGCCGGCC
FspI 856 TGCGCA TGC^GCA
965 TGCGCA
MseI 74 TTAA T^TAA
1517 TTAA
PflFI 934 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2908 GACTGTGTC
PleI 3211 GACTC GAGTC(4/5)
106 GAGTC
RcaI 44 TCATGA T^CATGA
803 TCATGA
SbfI 2292 CCTGCAGG CCTGCA^GG
3255 CCTGCAGG
Sse8387I 2292 CCTGCAGG CCTGCA^GG
3255 CCTGCAGG
StuI 1646 AGGCCT AGG^CCT
1889 AGGCCT
Tru9I 74 TTAA T^TAA
1517 TTAA
Tth111I 934 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2908 GACTGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI