Restriction map for
pF1KSDA0875
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BbeI BbsI
BcgI BclI BlnI BlpI Bpu1102I
BpuAI BsaHI BsaI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MluI NarI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 16 GTCGAC GT^[AC][GT]AC
AccIII 1580 TCCGGA T^CCGGA
AcsI 503 AAATTC [GA]^AATT[CT]
AflII 544 CTTAAG C^TTAAG
AhdI 571 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 503 AAATTC [GA]^AATT[CT]
AspEI 571 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 274 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1362 CCCGGG C^[CT]CG[GA]G
BalI 1315 TGGCCA TGG^CCA
BamHI 1577 GGATCC G^GATCC
BanII 1495 GGGCTC G[GA]GC[CT]^C
BbrPI 1108 CACGTG CAC^GTG
BbvCI 78 CCTCAGC CCTCAGC(-5/-2)
BfrI 544 CTTAAG C^TTAAG
BglI 727 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1108 CACGTG [CT]AC^GT[GA]
BsaBI 1237 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1580 TCCGGA T^CCGGA
BsmI 1084 GAATGC GAATGC(1/-1)
BsoBI 1362 CCCGGG C^[CT]CG[GA]G
BspEI 1580 TCCGGA T^CCGGA
BsrDI 1249 GCAATG GCAATG(2/0)
BssSI 1570 CACGAG CACGAG(-5/-1)
BstXI 749 CCATGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1547 GGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 571 GACTATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 292 GAAGAG CTCTTC(1/4)
EcoO109I 1547 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 999 CCAAGG C^C[AT][AT]GG
EcoT22I 868 ATGCAT ATGCA^T
HaeII 42 GGCGCT [GA]GCGC^[CT]
HgaI 1727 GACGC GACGC(5/10)
HindIII 473 AAGCTT A^AGCTT
Ksp632I 292 GAAGAG CTCTTC(1/4)
MamI 1237 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1315 TGGCCA TGG^CCA
MroI 1580 TCCGGA T^CCGGA
MscI 1315 TGGCCA TGG^CCA
NsiI 868 ATGCAT ATGCA^T
NspI 908 GCATGT [GA]CATG^[CT]
PflFI 274 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 777 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1108 CACGTG CAC^GTG
PmlI 1108 CACGTG CAC^GTG
PpuMI 1547 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1115 CTGCAG CTGCA^G
SalI 16 GTCGAC G^TCGAC
SmaI 1362 CCCGGG CCC^GGG
SspI 1828 AATATT AAT^ATT
StuI 548 AGGCCT AGG^CCT
StyI 999 CCAAGG C^C[AT][AT]GG
Tth111I 274 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 777 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1404 TCTAGA T^CTAGA
XcmI 1553 CCACCTGCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1362 CCCGGG C^CCGGG
XmaI 1362 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 370 GAGCAC G[AT]GC[AT]^C
1408 GAGCAC
BanI 36 GGTGCC G^G[CT][GA]CC
995 GGTGCC
BciVI 449 GTATCC GTATCC(6/5)
1788 GTATCC
BfaI 1357 CTAG C^TAG
1405 CTAG
BglII 530 AGATCT A^GATCT
1797 AGATCT
BsaWI 1580 TCCGGA [AT]^CCGG[AT]
1784 TCCGGT
BsiEI 143 CGGCCG CG[GA][CT]^CG
156 CGGCCG
BsiHKAI 370 GAGCAC G[AT]GC[AT]^C
1408 GAGCAC
BsrFI 39 GCCGGC [GA]^CCGG[CT]
176 GCCGGC
Cfr10I 39 GCCGGC [GA]^CCGG[CT]
176 GCCGGC
EagI 143 CGGCCG C^GGCCG
156 CGGCCG
EclXI 143 CGGCCG C^GGCCG
156 CGGCCG
Eco52I 143 CGGCCG C^GGCCG
156 CGGCCG
MaeI 1357 CTAG C^TAG
1405 CTAG
MfeI 282 CAATTG C^AATTG
929 CAATTG
MunI 282 CAATTG C^AATTG
929 CAATTG
NaeI 39 GCCGGC GCC^GGC
176 GCCGGC
NgoMIV 39 GCCGGC G^CCGGC
176 GCCGGC
PvuII 81 CAGCTG CAG^CTG
1069 CAGCTG
ScaI 611 AGTACT AGT^ACT
1091 AGTACT
SexAI 1262 ACCAGGT A^CC[AT]GGT
1388 ACCTGGT
SfcI 261 CTACAG C^T[GA][CT]AG
1115 CTGCAG
SmlI 544 CTTAAG C^T[CT][GA]AG
1700 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII