Restriction map for
pF1KSDA0871
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BciVI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HpaI HpaII KasI
KpnI KspI MaeII MaeIII MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AciI 428 CCGC CCGC(-3/-1)
AcyI 12 GACGCC G[GA]^CG[CT]C
AfaI 749 GTAC GT^AC
AflII 466 CTTAAG C^TTAAG
AlwNI 1186 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 510 ATTAAT AT^TAAT
AsnI 510 ATTAAT AT^TAAT
BalI 224 TGGCCA TGG^CCA
BanII 1205 GAGCTC G[GA]GC[CT]^C
BclI 548 TGATCA T^GATCA
BfaI 397 CTAG C^TAG
BfrI 466 CTTAAG C^TTAAG
BglII 453 AGATCT A^GATCT
BsaBI 1092 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 12 GACGCC G[GA]^CG[CT]C
BsaI 70 GAGACC GGTCTC(1/5)
BseRI 889 GAGGAG GAGGAG(10/8)
BsgI 1376 GTGCAG GTGCAG(16/14)
BsiEI 20 CGACCG CG[GA][CT]^CG
BsmBI 758 GAGACG CGTCTC(1/5)
BsmFI 989 GTCCC GGGAC(10/14)
BsmI 822 GAATGC GAATGC(1/-1)
DraI 1044 TTTAAA TTT^AAA
DraIII 1022 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 224 TGGCCA [CT]^GGCC[GA]
EcoRI 574 GAATTC G^AATTC
EcoT22I 857 ATGCAT ATGCA^T
FbaI 548 TGATCA T^GATCA
HgaI 12 GACGC GACGC(5/10)
Hin1I 12 GACGCC G[GA]^CG[CT]C
MaeI 397 CTAG C^TAG
MamI 1092 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 224 TGGCCA TGG^CCA
MscI 224 TGGCCA TGG^CCA
MspA1I 1189 CAGCTG C[AC]G^C[GT]G
NsiI 857 ATGCAT ATGCA^T
PpuMI 987 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 510 ATTAAT AT^TAAT
PvuII 1189 CAGCTG CAG^CTG
RsaI 749 GTAC GT^AC
SacI 1205 GAGCTC GAGCT^C
SapI 488 GAAGAGC GCTCTTC(1/4)
XbaI 396 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 88 AAATTC [GA]^AATT[CT]
574 GAATTC
ApoI 88 AAATTC [GA]^AATT[CT]
574 GAATTC
AspHI 325 GAGCAC G[AT]GC[AT]^C
1205 GAGCTC
AvaII 783 GGACC G^G[AT]CC
988 GGTCC
BbsI 679 GAAGAC GAAGAC(2/6)
1293 GAAGAC
BcgI 20 CGACCGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
582 CGAACCCAATGC
BmyI 325 GAGCAC G[AGT]GC[ACT]^C
1205 GAGCTC
BpuAI 679 GAAGAC GAAGAC(2/6)
1293 GAAGAC
BsiHKAI 325 GAGCAC G[AT]GC[AT]^C
1205 GAGCTC
BsiYI 383 CCTTCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
594 CCTCATGATGG
BslI 383 CCTTCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
594 CCTCATGATGG
Bsp1286I 325 GAGCAC G[AGT]GC[ACT]^C
1205 GAGCTC
BspHI 215 TCATGA T^CATGA
596 TCATGA
BstYI 177 AGATCC [GA]^GATC[CT]
453 AGATCT
DraII 390 GGGGCCT [GA]G^G[ACGT]CC[CT]
987 GGGTCCC
DsaI 65 CCATGG C^C[GA][CT]GG
101 CCATGG
EcoO109I 390 GGGGCCT [GA]G^G[ACGT]CC[CT]
987 GGGTCCC
EcoT14I 65 CCATGG C^C[AT][AT]GG
101 CCATGG
HaeIII 225 GGCC GG^CC
392 GGCC
HindIII 412 AAGCTT A^AGCTT
542 AAGCTT
HphI 318 GGTGA GGTGA(8/7)
1350 GGTGA
MflI 177 AGATCC [GA]^GATC[CT]
453 AGATCT
MslI 216 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
817 CATTTGAATG
MwoI 117 GCTCTGCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
616 GCCATAATTGC
NcoI 65 CCATGG C^CATGG
101 CCATGG
PstI 1007 CTGCAG CTGCA^G
1234 CTGCAG
RcaI 215 TCATGA T^CATGA
596 TCATGA
SfcI 1007 CTGCAG C^T[GA][CT]AG
1234 CTGCAG
StyI 65 CCATGG C^C[AT][AT]GG
101 CCATGG
TfiI 253 GAATC G^A[AT]TC
973 GAATC
XhoII 177 AGATCC [GA]^GATC[CT]
453 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI AlwI BbvI BpmI BsaJI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HinfI ItaI
Ksp632I MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SmlI
Tru9I TseI Tsp509I TspRI