Restriction map for
pF1KSDA0862
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BclI
BglI BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 513 GCGG CCGC(-3/-1)
AflII 1408 CTTAAG C^TTAAG
Alw44I 920 GTGCAC G^TGCAC
AlwNI 230 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 920 GTGCAC G^TGCAC
Asp700I 751 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 277 GAGCTC G[GA]GC[CT]^C
BciVI 1035 GTATCC GTATCC(6/5)
BcnI 291 CCGGG CC^[GC]GG
BfrI 1408 CTTAAG C^TTAAG
BglII 328 AGATCT A^GATCT
BlpI 657 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 657 GCTTAGC GC^T[ACGT]AGC
BsaI 1267 GGTCTC GGTCTC(1/5)
BsmAI 1268 GTCTC GTCTC(1/5)
BsmI 662 GCATTC GAATGC(1/-1)
Bsp1407I 778 TGTACA T^GTACA
BsrDI 257 GCAATG GCAATG(2/0)
BsrGI 778 TGTACA T^GTACA
BstEII 1471 GGTCACC G^GT[ACGT]ACC
BstPI 1471 GGTCACC G^GT[ACGT]ACC
BstXI 1323 CCATGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 657 GCTTAGC GC^T[ACGT]AGC
DraII 1689 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 220 CGGCCA [CT]^GGCC[GA]
EcoNI 756 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1689 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1471 GGTCACC G^GT[ACGT]ACC
EcoRI 300 GAATTC G^AATTC
HapII 291 CCGG C^CGG
HpaI 1170 GTTAAC GTT^AAC
HpaII 291 CCGG C^CGG
MspI 291 CCGG C^CGG
NciI 291 CCGGG CC^[GC]GG
NspI 653 GCATGC [GA]CATG^[CT]
PstI 1617 CTGCAG CTGCA^G
SacI 277 GAGCTC GAGCT^C
SphI 653 GCATGC GCATG^C
SspBI 778 TGTACA T^GTACA
XbaI 1362 TCTAGA T^CTAGA
XmnI 751 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 1252 CCTGAGG CC^T[ACGT]AGG
1338 CCTTAGG
AspHI 277 GAGCTC G[AT]GC[AT]^C
920 GTGCAC
BmyI 277 GAGCTC G[AGT]GC[ACT]^C
920 GTGCAC
BsiHKAI 277 GAGCTC G[AT]GC[AT]^C
920 GTGCAC
BsmFI 238 GGGAC GGGAC(10/14)
408 GTCCC
Bsp1286I 277 GAGCTC G[AGT]GC[ACT]^C
920 GTGCAC
BspHI 443 TCATGA T^CATGA
1634 TCATGA
BspMI 690 ACCTGC ACCTGC(4/8)
1586 ACCTGC
BstNI 184 CCAGG CC^[AT]GG
235 CCTGG
Bsu36I 1252 CCTGAGG CC^T[ACGT]AGG
1338 CCTTAGG
DsaI 1323 CCATGG C^C[GA][CT]GG
1739 CCATGG
Eco81I 1252 CCTGAGG CC^T[ACGT]AGG
1338 CCTTAGG
EcoRII 184 CCAGG ^CC[AT]GG
235 CCTGG
HindIII 1315 AAGCTT A^AGCTT
1624 AAGCTT
MvaI 184 CCAGG CC^[AT]GG
235 CCTGG
MwoI 510 GCTGCGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
653 GCATGCTTAGC
NcoI 1323 CCATGG C^CATGG
1739 CCATGG
NlaIV 1689 GGGGCC GG[ACGT]^[ACGT]CC
1725 GGGTCC
PflMI 229 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1039 CCAGTGGGTGG
RcaI 443 TCATGA T^CATGA
1634 TCATGA
SfcI 836 CTATAG C^T[GA][CT]AG
1617 CTGCAG
TaqI 666 TCGA T^CGA
1095 TCGA
Tsp45I 1472 GTCAC ^GT[GC]AC
1661 GTCAC
TthHB8I 666 TCGA T^CGA
1095 TCGA
Van91I 229 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1039 CCAGTGGGTGG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BpmI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII