Restriction map for
pF1KSDA0860
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbsI BbvCI BclI BlnI
BpuAI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HinfI HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MluNI MroI MscI MunI NarI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccII 429 CGCG CG^CG
AcsI 38 GAATTC [GA]^AATT[CT]
AflII 516 CTTAAG C^TTAAG
AflIII 1110 ACATGT A^C[GA][CT]GT
Alw44I 1569 GTGCAC G^TGCAC
ApaLI 1569 GTGCAC G^TGCAC
ApoI 38 GAATTC [GA]^AATT[CT]
BamHI 792 GGATCC G^GATCC
BanI 1405 GGCACC G^G[CT][GA]CC
BbrPI 505 CACGTG CAC^GTG
BcgI 842 GCAAGGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 551 GTATCC GTATCC(6/5)
BcnI 977 CCCGG CC^[GC]GG
BfrI 516 CTTAAG C^TTAAG
BglI 395 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 96 AGATCT A^GATCT
BlpI 313 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
BsaAI 505 CACGTG [CT]AC^GT[GA]
BsaBI 522 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 781 GAGGAG GAGGAG(10/8)
BsiEI 1585 CGGCCG CG[GA][CT]^CG
Bsp1407I 248 TGTACA T^GTACA
BspLU11I 1110 ACATGT A^CATGT
BsrGI 248 TGTACA T^GTACA
BssSI 1267 CACGAG CACGAG(-5/-1)
BstAPI 1571 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 633 GGTCACC G^GT[ACGT]ACC
BstPI 633 GGTCACC G^GT[ACGT]ACC
BstUI 429 CGCG CG^CG
CelII 313 GCTGAGC GC^T[ACGT]AGC
CfoI 428 GCGC GCG^C
DsaI 686 CCATGG C^C[GA][CT]GG
EagI 1585 CGGCCG C^GGCCG
EclXI 1585 CGGCCG C^GGCCG
Eco52I 1585 CGGCCG C^GGCCG
EcoNI 648 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 633 GGTCACC G^GT[ACGT]ACC
EcoRI 38 GAATTC G^AATTC
EcoRV 54 GATATC GAT^ATC
FauI 1611 GCGGG CCCGC(4/6)
HhaI 428 GCGC GCG^C
HinP1I 428 GCGC G^CGC
HindIII 1287 AAGCTT A^AGCTT
MamI 522 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 531 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 429 CGCG CG^CG
NaeI 588 GCCGGC GCC^GGC
NciI 977 CCCGG CC^[GC]GG
NcoI 686 CCATGG C^CATGG
NdeI 1237 CATATG CA^TATG
NgoMIV 588 GCCGGC G^CCGGC
NheI 1594 GCTAGC G^CTAGC
NspI 1110 ACATGT [GA]CATG^[CT]
PmaCI 505 CACGTG CAC^GTG
PmlI 505 CACGTG CAC^GTG
SacI 472 GAGCTC GAGCT^C
SspBI 248 TGTACA T^GTACA
StuI 1399 AGGCCT AGG^CCT
XcmI 1157 CCACAAGCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 249 GTAC GT^AC
1497 GTAC
ApaI 306 GGGCCC GGGCC^C
1437 GGGCCC
BfaI 266 CTAG C^TAG
1595 CTAG
DraII 415 AGGCCCC [GA]G^G[ACGT]CC[CT]
1437 GGGCCCC
EcoO109I 415 AGGCCCC [GA]G^G[ACGT]CC[CT]
1437 GGGCCCC
MaeI 266 CTAG C^TAG
1595 CTAG
PstI 667 CTGCAG CTGCA^G
745 CTGCAG
RsaI 249 GTAC GT^AC
1497 GTAC
SmlI 516 CTTAAG C^T[CT][GA]AG
970 CTCAAG
TaqI 851 TCGA T^CGA
1249 TCGA
Tsp509I 39 AATT ^AATT
1296 AATT
TthHB8I 851 TCGA T^CGA
1249 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AspHI AvaII BanII BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr10I Cfr13I DdeI
DpnII EaeI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp45I TspRI XhoII