Restriction map for
pF1KSDA0857
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MaeI MamI MfeI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SbfI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 358 GTCGAC GT^[AC][GT]AC
AflIII 128 ACGCGT A^C[GA][CT]GT
AlwNI 1870 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 719 TGCGCA TGC^GCA
BalI 1728 TGGCCA TGG^CCA
BamHI 654 GGATCC G^GATCC
BbvCI 776 CCTCAGC CCTCAGC(-5/-2)
BlpI 1845 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1845 GCTGAGC GC^T[ACGT]AGC
BsiEI 1890 CGACCG CG[GA][CT]^CG
BsmI 1697 GCATTC GAATGC(1/-1)
BstXI 801 CCAGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1845 GCTGAGC GC^T[ACGT]AGC
EcoNI 369 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 719 TGCGCA TGC^GCA
HincII 358 GTCGAC GT[CT]^[GA]AC
HindII 358 GTCGAC GT[CT]^[GA]AC
KspI 84 CCGCGG CCGC^GG
MluI 128 ACGCGT A^CGCGT
MluNI 1728 TGGCCA TGG^CCA
MscI 1728 TGGCCA TGG^CCA
NaeI 24 GCCGGC GCC^GGC
NgoMIV 24 GCCGGC G^CCGGC
NspI 1863 GCATGT [GA]CATG^[CT]
SacII 84 CCGCGG CCGC^GG
SalI 358 GTCGAC G^TCGAC
SapI 998 GCTCTTC GCTCTTC(1/4)
ScaI 1787 AGTACT AGT^ACT
SfiI 1239 GGCCCCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1944 CCCGGG CCC^GGG
SmlI 1533 CTTGAG C^T[CT][GA]AG
StuI 1410 AGGCCT AGG^CCT
TfiI 616 GAATC G^A[AT]TC
XmaCI 1944 CCCGGG C^CCGGG
XmaI 1944 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AspI 394 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
735 GACCCAGTC
BbeI 285 GGCGCC GGCGC^C
811 GGCGCC
BbsI 183 GAAGAC GAAGAC(2/6)
400 GTCTTC
BpuAI 183 GAAGAC GAAGAC(2/6)
400 GTCTTC
BsrFI 24 GCCGGC [GA]^CCGG[CT]
1892 ACCGGC
BstYI 654 GGATCC [GA]^GATC[CT]
1937 AGATCC
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
1892 ACCGGC
DraIII 426 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1573 CACCCCGTG
DsaI 84 CCGCGG C^C[GA][CT]GG
380 CCACGG
EaeI 1728 TGGCCA [CT]^GGCC[GA]
1781 CGGCCA
KasI 285 GGCGCC G^GCGCC
811 GGCGCC
MaeII 59 ACGT A^CGT
169 ACGT
MflI 654 GGATCC [GA]^GATC[CT]
1937 AGATCC
MslI 330 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
979 CACCTGGATG
NarI 285 GGCGCC GG^CGCC
811 GGCGCC
PflFI 394 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
735 GACCCAGTC
PflMI 1087 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1731 CCAGATGGTGG
PstI 1828 CTGCAG CTGCA^G
1933 CTGCAG
PvuII 846 CAGCTG CAG^CTG
1763 CAGCTG
SfaNI 936 GATGC GCATC(5/9)
985 GATGC
SfoI 285 GGCGCC GGC^GCC
811 GGCGCC
Tth111I 394 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
735 GACCCAGTC
Van91I 1087 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1731 CCAGATGGTGG
XhoII 654 GGATCC [GA]^GATC[CT]
1937 AGATCC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI SacI Sau3AI Sau96I ScrFI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI