Restriction map for
pF1KSDA0851
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI AscI Asp718I
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstUI BstYI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI KspI MflI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 176 GTATAC GT^[AC][GT]AC
AhdI 100 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 730 GTGCAC G^TGCAC
ApaLI 730 GTGCAC G^TGCAC
AseI 601 ATTAAT AT^TAAT
AsnI 601 ATTAAT AT^TAAT
AspEI 100 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1122 TGGCCA TGG^CCA
BanI 1357 GGTGCC G^G[CT][GA]CC
BanII 933 GGGCTC G[GA]GC[CT]^C
BcgI 1156 CGAAGCAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 624 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 530 CTGCAC GTGCAG(16/14)
BsmI 588 GCATTC GAATGC(1/-1)
Bsp1407I 759 TGTACA T^GTACA
BspMI 202 GCAGGT ACCTGC(4/8)
BsrDI 1005 CATTGC GCAATG(2/0)
BsrGI 759 TGTACA T^GTACA
Bst1107I 176 GTATAC GTA^TAC
BstEII 117 GGTTACC G^GT[ACGT]ACC
BstPI 117 GGTTACC G^GT[ACGT]ACC
BstXI 1546 CCAAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 176 GTATAC GTA^TAC
CfoI 663 GCGC GCG^C
EaeI 1122 TGGCCA [CT]^GGCC[GA]
Eam1105I 100 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1660 CTCTTC CTCTTC(1/4)
EcoO65I 117 GGTTACC G^GT[ACGT]ACC
EcoRI 454 GAATTC G^AATTC
EcoT22I 586 ATGCAT ATGCA^T
FauI 1348 GCGGG CCCGC(4/6)
HhaI 663 GCGC GCG^C
HinP1I 663 GCGC G^CGC
Ksp632I 1660 CTCTTC CTCTTC(1/4)
MaeII 86 ACGT A^CGT
MamI 624 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1161 CAATTG C^AATTG
MluNI 1122 TGGCCA TGG^CCA
MscI 1122 TGGCCA TGG^CCA
MslI 733 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1161 CAATTG C^AATTG
NdeI 407 CATATG CA^TATG
NsiI 586 ATGCAT ATGCA^T
NspI 1254 GCATGT [GA]CATG^[CT]
PshBI 601 ATTAAT AT^TAAT
SapI 1659 GCTCTTC GCTCTTC(1/4)
SpeI 445 ACTAGT A^CTAGT
SspBI 759 TGTACA T^GTACA
SspI 168 AATATT AAT^ATT
XbaI 1176 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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Asp700I 680 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1499 GAAACTATTC
AspHI 730 GTGCAC G[AT]GC[AT]^C
1657 GTGCTC
AvaI 633 CTCGAG C^[CT]CG[GA]G
767 CTCGAG
AvaII 791 GGTCC G^G[AT]CC
1740 GGTCC
BseRI 636 GAGGAG GAGGAG(10/8)
639 GAGGAG
BsiHKAI 730 GTGCAC G[AT]GC[AT]^C
1657 GTGCTC
BsmAI 1239 GAGAC GTCTC(1/5)
470 GTCTC
BsoBI 633 CTCGAG C^[CT]CG[GA]G
767 CTCGAG
Cac8I 1120 GCTGGC GC[ACGT]^[ACGT]GC
1675 GCAAGC
DdeI 517 CTAAG C^T[ACGT]AG
1054 CTAAG
EcoT14I 980 CCTTGG C^C[AT][AT]GG
1546 CCAAGG
HphI 244 GGTGA GGTGA(8/7)
1627 GGTGA
NlaIV 791 GGTCCC GG[ACGT]^[ACGT]CC
1357 GGTGCC
PaeR7I 633 CTCGAG C^TCGAG
767 CTCGAG
PvuII 22 CAGCTG CAG^CTG
152 CAGCTG
SfaNI 360 GATGC GCATC(5/9)
1726 GATGC
StyI 980 CCTTGG C^C[AT][AT]GG
1546 CCAAGG
Tsp45I 157 GTCAC ^GT[GC]AC
1628 GTGAC
XhoI 633 CTCGAG C^TCGAG
767 CTCGAG
XmnI 680 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1499 GAAACTATTC
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HindIII HinfI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I Van91I