Restriction map for
pF1KSDA0833
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# NO CUT ENZYMES ( 63 enzymes) #
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AatII Acc65I AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII BamHI BbeI BbvCI
BciVI BfrI BspDI BspLU11I Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eco47III EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI SfoI
SfuI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 3990 GTCTAC GT^[AC][GT]AC
AccIII 4604 TCCGGA T^CCGGA
Alw44I 529 GTGCAC G^TGCAC
AocI 4265 CCTCAGG CC^T[ACGT]AGG
ApaI 2432 GGGCCC GGGCC^C
ApaLI 529 GTGCAC G^TGCAC
BbrPI 1981 CACGTG CAC^GTG
BbsI 3665 GAAGAC GAAGAC(2/6)
BcgI 161 CGAAAAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 3665 GAAGAC GAAGAC(2/6)
BsaAI 1981 CACGTG [CT]AC^GT[GA]
BsaBI 2928 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4604 TCCGGA T^CCGGA
BsiEI 2558 CGGCCG CG[GA][CT]^CG
BsiWI 1490 CGTACG C^GTACG
BsmBI 1987 GAGACG CGTCTC(1/5)
BsmI 4153 GAATGC GAATGC(1/-1)
BspEI 4604 TCCGGA T^CCGGA
BspHI 4554 TCATGA T^CATGA
BsrDI 689 GCAATG GCAATG(2/0)
BssHII 6 GCGCGC G^CGCGC
BssSI 937 CACGAG CACGAG(-5/-1)
Bsu36I 4265 CCTCAGG CC^T[ACGT]AGG
DraIII 3062 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2558 CGGCCG C^GGCCG
EclXI 2558 CGGCCG C^GGCCG
Eco52I 2558 CGGCCG C^GGCCG
Eco81I 4265 CCTCAGG CC^T[ACGT]AGG
EcoNI 1581 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 3301 GTTGAC GT[CT]^[GA]AC
HindII 3301 GTTGAC GT[CT]^[GA]AC
MamI 2928 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4604 TCCGGA T^CCGGA
PmaCI 1981 CACGTG CAC^GTG
PmlI 1981 CACGTG CAC^GTG
PshAI 2785 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4554 TCATGA T^CATGA
SbfI 1851 CCTGCAGG CCTGCA^GG
SexAI 1040 ACCAGGT A^CC[AT]GGT
SfiI 2559 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2187 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2585 GCATGC GCATG^C
Sse8387I 1851 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AhdI 1780 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2896 GACTGGCTGTC
AspEI 1780 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2896 GACTGGCTGTC
AspI 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2979 GACGGGGTC
AvrII 2580 CCTAGG C^CTAGG
3357 CCTAGG
BglII 2723 AGATCT A^GATCT
3972 AGATCT
BlnI 2580 CCTAGG C^CTAGG
3357 CCTAGG
Eam1105I 1780 GACTACACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2896 GACTGGCTGTC
EcoRI 3963 GAATTC G^AATTC
4489 GAATTC
MseI 250 TTAA T^TAA
1466 TTAA
NspI 2534 ACATGC [GA]CATG^[CT]
2585 GCATGC
PflFI 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2979 GACGGGGTC
ScaI 3752 AGTACT AGT^ACT
4805 AGTACT
StuI 1503 AGGCCT AGG^CCT
1949 AGGCCT
Tru9I 250 TTAA T^TAA
1466 TTAA
Tth111I 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2979 GACGGGGTC
XcmI 1034 CCACCAACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1730 CCAGCACCACCCTGG
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# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspBI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI